Gigaxonin (GAN) (NM_022041) Human Tagged ORF Clone

CAT#: RC201669

GAN (Myc-DDK-tagged)-Human gigaxonin (GAN)

Product Images

Circular map for RC201669

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gigaxonin
• Synonyms: GAN1; GIG; KLHL16
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201669 representing NM_022041
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGAGGGCAGTGCCGTGTCTGACCCTCAGCACGCCGCGCGTCTGCTGCGAGCGCTCAGCTCTTTCC
  GCGAGGAGTCTCGCTTCTGCGACGCGCACCTGGTCCTCGACGGGGAGGAGATCCCGGTGCAGAAGAACAT
  CCTGGCGGCGGCCAGCCCGTACATCAGGACAAAGTTAAACTATAATCCTCCAAAAGATGATGGATCAACT
  TATAAGATTGAACTTGAAGGGATATCGGTAATGGTTATGAGAGAGATCCTGGATTACATCTTCAGTGGGC
  AGATCAGGCTAAATGAAGATACAATCCAAGATGTTGTTCAGGCAGCTGACCTGCTGCTACTGACGGACCT
  TAAAACCCTGTGCTGTGAGTTTTTGGAAGGCTGCATTGCTGCTGAGAACTGTATTGGTATCCGTGACTTT
  GCACTACATTACTGCCTCCATCACGTTCATTACCTTGCCACAGAATACCTGGAGACTCATTTCCGAGACG
  TCAGCAGCACGGAAGAATTCTTAGAGCTGAGTCCTCAAAAGCTTAAAGAAGTGATTTCTCTTGAGAAGTT
  AAACGTTGGCAATGAAAGATATGTCTTTGAAGCAGTAATTCGATGGATAGCACATGATACAGAAATAAGA
  AAGGTCCACATGAAGGATGTTATGTCAGCTCTGTGGGTTTCAGGGTTGGACTCCAGTTATTTACGGGAAC
  AGATGCTGAATGAACCATTAGTACGAGAAATTGTCAAAGAGTGTAGCAATATACCGCTCAGCCAGCCGCA
  GCAAGGGGAGGCGATGCTGGCCAACTTCAAACCCCGGGGCTACTCTGAGTGCATCGTGACTGTTGGTGGA
  GAAGAGAGAGTTTCACGGAAACCCACAGCAGCGATGCGATGCATGTGCCCTCTCTATGACCCTAACAGGC
  AGCTTTGGATCGAACTGGCCCCTTTAAGCATGCCGAGAATTAACCATGGAGTTCTCTCAGCAGAAGGATT
  TTTGTTTGTATTCGGGGGCCAAGATGAAAATAAGCAGACTCTTAGCTCAGGAGAAAAGTATGATCCAGAT
  GCAAATACATGGACAGCATTGCCACCTATGAACGAGGCAAGACATAACTTCGGAATTGTGGAGATAGATG
  GGATGCTGTACATTTTGGGAGGAGAGGATGGTGAAAAGGAGCTGATTTCCATGGAGTGTTACGATATTTA
  TTCTAAAACCTGGACAAAGCAACCTGATTTGACCATGGTCAGAAAGATCGGCTGCTATGCAGCTATGAAA
  AAGAAAATCTACGCCATGGGTGGAGGCTCCTACGGAAAGCTTTTTGAGTCTGTAGAGTGTTATGATCCCA
  GGACCCAGCAGTGGACTGCCATATGTCCACTAAAAGAGAGGAGGTTTGGAGCGGTGGCCTGTGGAGTTGC
  TATGGAGCTGTATGTGTTTGGGGGAGTCCGAAGTCGTGAGGACGCCCAGGGTAGCGAGATGGTAACTTGC
  AAGTCCGAGTTCTACCATGATGAGTTTAAAAGGTGGATCTATCTTAACGACCAGAATTTATGCATCCCCG
  CCAGTTCCTCTTTTGTTTATGGAGCTGTACCTATAGGAGCCAGTATTTATGTTATTGGAGATCTTGATAC
  AGGTACCAATTACGACTACGTGCGTGAGTTTAAAAGAAGCACAGGAACCTGGCACCACACTAAACCACTC
  CTTCCATCCGACCTTCGCCGTACAGGATGTGCAGCCTTACGCATTGCGAATTGCAAGCTTTTCCGCCTGC
  AGCTTCAGCAAGGCTTATTCCGTATTCGTGTTCATTCCCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201669 representing NM_022041
  Red=Cloning site Green=Tags(s)
  
  MAEGSAVSDPQHAARLLRALSSFREESRFCDAHLVLDGEEIPVQKNILAAASPYIRTKLNYNPPKDDGST
  YKIELEGISVMVMREILDYIFSGQIRLNEDTIQDVVQAADLLLLTDLKTLCCEFLEGCIAAENCIGIRDF
  ALHYCLHHVHYLATEYLETHFRDVSSTEEFLELSPQKLKEVISLEKLNVGNERYVFEAVIRWIAHDTEIR
  KVHMKDVMSALWVSGLDSSYLREQMLNEPLVREIVKECSNIPLSQPQQGEAMLANFKPRGYSECIVTVGG
  EERVSRKPTAAMRCMCPLYDPNRQLWIELAPLSMPRINHGVLSAEGFLFVFGGQDENKQTLSSGEKYDPD
  ANTWTALPPMNEARHNFGIVEIDGMLYILGGEDGEKELISMECYDIYSKTWTKQPDLTMVRKIGCYAAMK
  KKIYAMGGGSYGKLFESVECYDPRTQQWTAICPLKERRFGAVACGVAMELYVFGGVRSREDAQGSEMVTC
  KSEFYHDEFKRWIYLNDQNLCIPASSSFVYGAVPIGASIYVIGDLDTGTNYDYVREFKRSTGTWHHTKPL
  LPSDLRRTGCAALRIANCKLFRLQLQQGLFRIRVHSP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022041
• ORF Size: 1791 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022041.4
• RefSeq Size: 4544 bp
• RefSeq ORF: 1794 bp
• Locus ID: 8139
• UniProt ID: Q9H2C0
• Cytogenetics: 16q23.2
• Domains: BTB, Kelch
• Protein Families: Druggable Genome
• MW: 67.6 kDa
• Gene Summary: This gene encodes a member of the cytoskeletal BTB/kelch (Broad-Complex, Tramtrack and Bric a brac) repeat family. The encoded protein plays a role in neurofilament architecture and is involved in mediating the ubiquitination and degradation of some proteins. Defects in this gene are a cause of giant axonal neuropathy (GAN). [provided by RefSeq, Oct 2008]