CEP72 (NM_018140) Human Tagged ORF Clone

CAT#: RC201663

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CEP72 (Myc-DDK-tagged)-Human centrosomal protein 72kDa (CEP72)



Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 477.00


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In Stock

Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol CEP72
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC201663 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGCGGGCTGGCCCTCGGCTGGTGCTGAGCGAGGAGGCGGTTCGGGCGAAGAGCGGCTTAGGGCCTC
ACCGCGACCTGGCTGAGCTTCAGTCATTGTCTATTCCTGGAACTTACCAAGAGAAGATCACCCACCTGGG
ACATTCTCTGATGAGTTTAACAGGTCTGAAATCTTTGGATCTCTCGCGCAACTCCTTGGTTAGTCTGGAG
GGCATTCAGTACCTGACTGCATTGGAGAGTCTCAATCTCTACTACAACTGCATCTCCTCGTTGGCAGAAG
TGTTTCGGCTCCACGCCTTAACCGAGCTCGTGGATGTGGACTTCCGGCTGAACCCCGTGGTGAAGGTTGA
GCCTGACTACCGCCTTTTTGTTGTGCACCTGCTCCCCAAGCTCCAGCAGCTGGACGATCGCCCCGTGAGA
GCAAGCGAGCGGAAGGCTTCCCGACTGCATTTTGCATCAGAGGACTCACTCGACTCCAAAGAGAGCGTCC
CAGCTTCTTTGAAAGAGGGCAGACCACACCACCCCAGAGCCAAGTGCACCGAGGCCTTGGCCAAGCAGAG
CCTGGTCATGGATGCGGATGACGAGGCAGTCCTGAACCTCATTGCAGAGTGCGAGTGGGACCTCGGCAGG
CCTCCCGGGAGCACGAGCTTCAGCCAGAAGGGGCGTGAGGCCGACTCTCGTGGTTCCCAAGAATCCAGAC
ATCTGTTGAGCCCGCAGTTGGTACAGTACCAGTGTGGGGACTCTGGGAAGCAGGGCCGTGAGACGAGGAG
GAGCAGCTGCAGAGGGTGCTGTCTGGAGAAGATGCCTTGGAGCCAGCTCTGTGGAGAGCTTCCGCCACTG
TACGGAGCGGAGCCAGAGGCCTCCCGTGCCCCCAGGCCACACACGTACTTCACCCCACACCCAGACTCCA
TGGATACCGAGGACTCGGCCTCTTCTCAGAAGTTGGATTTGTCAGGAGAAATGGTGCCTGGTCCCCTGCC
AGCCCCCGGAAAGTGCAGGAAGCGAAGAATGCCTGTTGGAAGATTCCAGACGTTTTCGGACCAGGAGGGT
TTGGGCTGCCCGGAGAGAACTCATGGGTCCTCCGTGCCCAAGGAGAGCCTGAGCAGACAGGACAGCTCAG
AAAGCAGGAACGGGAGGACCTTGTCTCAGCCTGAGGCCTCGGAGACTGAGGAGCAGAGGTCTCGGGGTGT
GACCGACACCAGAGAGCCGTCTCCCGGGTCACACTCGGCTCTACCCGGGAAGAAGACGGCCCTGCAGGCG
GCGCTCCTGGAGACGCTCTTGGACCTGGTGGACAGGAGCTGGGGCGGCTGCAGGTCCCTGCACAGCAACG
AGGCATTCCTTGCTCAGGCAAGACACATCTTGTCATCTGTTGAAGAATTCACAGCAGCTCAGGACAGCTC
TGCGATGGTGGGTGAAGATGTCGGCTCCCTGGCTCTGGAGAGTAAGTCCCTGCAAAGCCGCCTTGCTGAG
CAGCAGCAGCAGCACGCCCGGGAGATGAGCGAGGTGACGGCGGAGCTGCACCACACACACAAGGAGCTGG
ATGATTTGAGACAACATTTAGATAAATCTTTGGAAGAGAACAGTAGGTTAAAATCGCTTTTGTTGAGTAT
GAAAAAGGAAGTGAAGAGTGCAGACACTGCAGCCACGTTAAATTTGCAGATCGCTGGACTTCAAACAAGT
GTGAAGAGGCTGTGTGGCGAGATTGTGGAACTGAAGCAGCACCTGGAGCACTACGACAAGATCCAGGAGC
TCACGCAGATGCTGCAGGAGAGCCACAGCTCCCTGGTCAGCACCAATGAACACCTGCTGCAGGAGCTGAG
CCAGGTGCGGGCGCAGCACAGAGCCGAGGTGGAGCAGATGCACTGGAGCTACCAGGAGCTCAAGAAGACC
ATGGCCCTGTTTCCACACAGCAGCGCCAGCCATGGAGGCTGCCAGGCCTGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC201663 protein sequence
Red=Cloning site Green=Tags(s)

MARAGPRLVLSEEAVRAKSGLGPHRDLAELQSLSIPGTYQEKITHLGHSLMSLTGLKSLDLSRNSLVSLE
GIQYLTALESLNLYYNCISSLAEVFRLHALTELVDVDFRLNPVVKVEPDYRLFVVHLLPKLQQLDDRPVR
ASERKASRLHFASEDSLDSKESVPASLKEGRPHHPRAKCTEALAKQSLVMDADDEAVLNLIAECEWDLGR
PPGSTSFSQKGREADSRGSQESRHLLSPQLVQYQCGDSGKQGRETRRSSCRGCCLEKMPWSQLCGELPPL
YGAEPEASRAPRPHTYFTPHPDSMDTEDSASSQKLDLSGEMVPGPLPAPGKCRKRRMPVGRFQTFSDQEG
LGCPERTHGSSVPKESLSRQDSSESRNGRTLSQPEASETEEQRSRGVTDTREPSPGSHSALPGKKTALQA
ALLETLLDLVDRSWGGCRSLHSNEAFLAQARHILSSVEEFTAAQDSSAMVGEDVGSLALESKSLQSRLAE
QQQQHAREMSEVTAELHHTHKELDDLRQHLDKSLEENSRLKSLLLSMKKEVKSADTAATLNLQIAGLQTS
VKRLCGEIVELKQHLEHYDKIQELTQMLQESHSSLVSTNEHLLQELSQVRAQHRAEVEQMHWSYQELKKT
MALFPHSSASHGGCQAC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_018140
ORF Size 1941 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_018140.4
RefSeq Size 2427 bp
RefSeq ORF 1944 bp
Locus ID 55722
UniProt ID Q9P209
Cytogenetics 5p15.33
MW 71.7 kDa
Gene Summary The product of this gene is a member of the leucine-rich-repeat (LRR) superfamily of proteins. The protein is localized to the centrosome, a non-membraneous organelle that functions as the major microtubule-organizing center in animal cells. [provided by RefSeq, Jul 2008]
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