RTF2 (NM_016407) Human Tagged ORF Clone
CAT#: RC201652
RTFDC1 (Myc-DDK-tagged)-Human chromosome 20 open reading frame 43 (C20orf43)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_016407" in other vectors (5)
USD 198.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | RTF2 |
Synonyms | C20orf43; CDAO5; HSPC164; RTFDC1; SHUJUN-3 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC201652 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTTGCGACGGGGGAACAATCCCCAAGAGGCATGAACTGGTGAAGGGGCCGAAGAAGGTTGAGAAGG TCGACAAAGATGCTGAATTAGTGGCCCAATGGAACTATTGTACTCTAAGTCAGGAAATATTAAGACGACC AATAGTTGCCTGTGAACTTGGCAGACTTTATAACAAAGATGCCGTCATTGAATTTCTCTTGGACAAATCT GCAGAAAAGGCTCTTGGGAAGGCAGCATCTCACATTAAAAGCATTAAGAATGTGACAGAGCTGAAGCTTT CTGATAATCCTGCCTGGGAAGGGGATAAAGGAAACACTAAAGGTGACAAGCACGATGACCTCCAGCGGGC GCGTTTCATCTGCCCCGTTGTGGGCCTGGAGATGAACGGCCGACACAGGTTCTGCTTCCTTCGGTGCTGC GGCTGTGTGTTTTCTGAGCGAGCCTTGAAAGAGATAAAAGCGGAAGTTTGCCACACGTGTGGGGCTGCCT TCCAGGAGGATGATGTCATCGTGCTCAATGGCACCAAGGAGGATGTGGACGTGCTGAAGACAAGGATGGA GGAGAGAAGGCTGAGAGCGAAGCTGGAAAAGAAAACAAAGAAACCCAAGGCAGCAGAGTCTGTTTCAAAA CCAGATGTCAGTGAAGAAGCCCCAGGGCCATCAAAAGTTAAGACAGGGAAGCCTGAAGAAGCCAGCCTTG ATTCTAGAGAGAAGAAAACCAACTTGGCTCCCAAAAGCACAGCAATGAATGAGAGCTCTTCTGGAAAAGC TGGGAAGCCTCCGTGTGGAGCCACAAAGAGGTCCATCGCTGACAGTGAAGAATCGGAGGCCTACAAGTCC CTCTTTACCACTCACAGCTCCGCCAAGCGCTCCAAGGAGGAGTCTGCCCACTGGGTCACCCACACGTCCT ACTGCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201652 protein sequence
Red=Cloning site Green=Tags(s) MGCDGGTIPKRHELVKGPKKVEKVDKDAELVAQWNYCTLSQEILRRPIVACELGRLYNKDAVIEFLLDKS AEKALGKAASHIKSIKNVTELKLSDNPAWEGDKGNTKGDKHDDLQRARFICPVVGLEMNGRHRFCFLRCC GCVFSERALKEIKAEVCHTCGAAFQEDDVIVLNGTKEDVDVLKTRMEERRLRAKLEKKTKKPKAAESVSK PDVSEEAPGPSKVKTGKPEEASLDSREKKTNLAPKSTAMNESSSGKAGKPPCGATKRSIADSEESEAYKS LFTTHSSAKRSKEESAHWVTHTSYCF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_016407 |
ORF Size | 918 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_016407.5 |
RefSeq Size | 1672 bp |
RefSeq ORF | 921 bp |
Locus ID | 51507 |
UniProt ID | Q9BY42 |
Cytogenetics | 20q13.31 |
Domains | DUF602 |
MW | 33.9 kDa |
Gene Summary | Replication termination factor which is a component of the elongating replisome (Probable). Required for ATR pathway signaling upon DNA damage and has a positive activity during DNA replication. Might function to facilitate fork pausing at replication fork barriers like the rDNA. May be globally required to stimulate ATR signaling after the fork stalls or encounters a lesion (Probable). Interacts with nascent DNA (PubMed:29290612).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC201652L3 | Lenti ORF clone of Human chromosome 20 open reading frame 43 (C20orf43), Myc-DDK-tagged |
USD 600.00 |
|
RC201652L4 | Lenti ORF clone of Human chromosome 20 open reading frame 43 (C20orf43), mGFP tagged |
USD 600.00 |
|
RG201652 | RTFDC1 (tGFP-tagged) - Human chromosome 20 open reading frame 43 (C20orf43) |
USD 500.00 |
|
SC114290 | RTFDC1 (untagged)-Human chromosome 20 open reading frame 43 (C20orf43) |
USD 300.00 |
|
SC322173 | RTFDC1 (untagged)-Human chromosome 20 open reading frame 43 (C20orf43) |
USD 300.00 |
{0} Product Review(s)
Be the first one to submit a review