c-Myc (MYC) (NM_002467) Human Tagged ORF Clone
Lenti ORF clone of Human v-myc myelocytomatosis viral oncogene homolog (avian) (MYC), Myc-DDK-tagged
|Product Name||c-Myc (MYC) (NM_002467) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|Synonyms||bHLHe39; c-Myc; MRTL; MYCC|
|E. coli Selection||Chloramphenicol (34 ug/mL)|
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene|
|ORF Size||1362 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||2379 bp|
|RefSeq ORF||1365 bp|
|Domains||HLH, Myc_N_term, Myc-LZ|
|Protein Families||Druggable Genome, Embryonic stem cells, Induced pluripotent stem cells, Stem cell - Pluripotency, Stem cell relevant signaling - JAK/STAT signaling pathway, Stem cell relevant signaling - TGFb/BMP signaling pathway, Stem cell relevant signaling - Wnt Signaling pathway, Transcription Factors|
|Protein Pathways||Acute myeloid leukemia, Bladder cancer, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Jak-STAT signaling pathway, MAPK signaling pathway, Pathways in cancer, Small cell lung cancer, TGF-beta signaling pathway, Thyroid cancer, Wnt signaling pathway|
|Gene Summary||This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini. [provided by RefSeq, Aug 2017]|
|cDNA Clone Resources|
|SC112715||MYC (untagged)-Human v-myc myelocytomatosis viral oncogene homolog (avian) (MYC)||
|RC201611||MYC (Myc-DDK-tagged)-Human v-myc myelocytomatosis viral oncogene homolog (avian) (Myc)||
|RG201611||MYC (GFP-tagged) - Human v-myc myelocytomatosis viral oncogene homolog (avian) (MYC)||
|RC201611L2||Lenti ORF clone of Human v-myc myelocytomatosis viral oncogene homolog (avian) (MYC), mGFP tagged||
|RC201611L3||Lenti ORF clone of Human v-myc myelocytomatosis viral oncogene homolog (avian) (MYC), Myc-DDK-tagged||
|RC201611L4||Lenti ORF clone of Human v-myc myelocytomatosis viral oncogene homolog (avian) (MYC), mGFP tagged||