HNRPM (HNRNPM) (NM_005968) Human Tagged ORF Clone

CAT#: RC201584

HNRNPM (Myc-DDK-tagged)-Human heterogeneous nuclear ribonucleoprotein M (HNRNPM), transcript variant 1

Product Images

Circular map for RC201584

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HNRPM
• Synonyms: CEAR; hnRNP M; HNRNPM4; HNRPM; HNRPM4; HTGR1; NAGR1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201584 representing NM_005968
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCAGGGGTCGAAGCGGCGGCGGAGGTGGCGGCGACGGAGATCAAAATGGAGGAAGAGAGCGGCG
  CGCCCGGCGTGCCGAGCGGCAACGGGGCTCCGGGCCCTAAGGGTGAAGGAGAACGACCTGCTCAGAATGA
  GAAGAGGAAGGAGAAAAACATAAAAAGAGGAGGCAATCGCTTTGAGCCATATGCCAATCCAACTAAAAGA
  TACAGAGCCTTCATTACAAACATACCTTTTGATGTGAAATGGCAGTCACTTAAAGACCTGGTTAAAGAAA
  AAGTTGGTGAGGTAACATACGTGGAGCTCTTAATGGACGCTGAAGGAAAGTCAAGGGGATGTGCTGTTGT
  TGAATTCAAGATGGAAGAGAGCATGAAAAAAGCTGCGGAAGTCCTAAACAAGCATAGTCTGAGCGGAAGA
  CCACTGAAAGTCAAAGAAGATCCTGATGGTGAACATGCCAGGAGAGCAATGCAAAAGGTGATGGCTACGA
  CTGGTGGGATGGGTATGGGACCAGGTGGCCCAGGAATGATTACTATCCCACCCAGTATCCTAAATAATCC
  CAACATCCCAAATGAGATTATCCATGCATTACAGGCTGGAAGACTTGGAAGCACAGTATTTGTAGCAAAT
  CTGGATTATAAAGTTGGCTGGAAGAAACTGAAGGAAGTATTTAGTATGGCTGGTGTGGTGGTCCGAGCAG
  ACATTCTTGAAGATAAAGATGGAAAAAGTCGTGGAATAGGCACTGTTACTTTTGAACAGTCCATTGAAGC
  TGTGCAAGCTATATCTATGTTCAATGGCCAGCTGCTATTTGATAGACCAATGCACGTCAAGATGGATGAG
  AGGGCCTTACCAAAAGGAGATTTCTTCCCTCCTGAGCGTCCACAACAACTTCCCCATGGCCTTGGTGGTA
  TTGGCATGGGGTTAGGACCAGGAGGGCAACCCATTGATGCCAATCACCTGAATAAAGGCATCGGAATGGG
  AAACATAGGTCCCGCAGGAATGGGAATGGAAGGCATAGGATTTGGAATAAATAAAATGGGAGGAATGGAG
  GGGCCCTTTGGTGGTGGTATGGAAAACATGGGTCGATTTGGATCTGGGATGAACATGGGCAGGATAAATG
  AAATCCTAAGTAATGCACTGAAGAGAGGAGAGATCATTGCAAAGCAGGGAGGAGGTGGAGGTGGAGGAAG
  CGTCCCTGGGATCGAGAGGATGGGTCCTGGCATTGACCGCCTCGGGGGTGCCGGCATGGAGCGCATGGGC
  GCGGGCCTGGGCCACGGCATGGATCGCGTGGGCTCCGAGATCGAGCGCATGGGCCTGGTCATGGACCGCA
  TGGGCTCCGTGGAGCGCATGGGCTCCGGCATTGAGCGCATGGGCCCGCTGGGCCTCGACCACATGGCCTC
  CAGCATTGAGCGCATGGGCCAGACCATGGAGCGCATTGGCTCTGGCGTGGAGCGCATGGGTGCCGGCATG
  GGCTTCGGCCTTGAGCGCATGGCCGCTCCCATCGACCGTGTGGGCCAGACCATTGAGCGCATGGGCTCTG
  GCGTGGAGCGCATGGGCCCTGCCATCGAGCGCATGGGCCTGAGCATGGAGCGCATGGTGCCCGCAGGTAT
  GGGAGCTGGCCTGGAGCGCATGGGCCCCGTGATGGATCGCATGGCCACCGGCCTGGAGCGCATGGGCGCC
  AACAATCTGGAGCGGATGGGCCTGGAGCGCATGGGCGCCAACAGCCTCGAGCGCATGGGCCTGGAGCGCA
  TGGGTGCCAACAGCCTCGAGCGCATGGGCCCTGCCATGGGCCCGGCCCTGGGCGCTGGCATTGAGCGCAT
  GGGCCTGGCCATGGGTGGCGGTGGCGGTGCCAGCTTTGACCGTGCCATCGAGATGGAGCGTGGCAACTTC
  GGAGGAAGCTTCGCAGGTTCCTTTGGTGGAGCTGGAGGCCATGCTCCTGGGGTGGCCAGGAAGGCCTGCC
  AGATATTTGTGAGAAATCTGCCATTCGATTTCACATGGAAGATGCTAAAGGACAAATTCAACGAGTGCGG
  CCACGTGCTGTACGCCGACATCAAGATGGAGAATGGGAAGTCCAAGGGGTGTGGCGTGGTTAAGTTCGAG
  TCGCCAGAGGTGGCCGAGAGAGCCTGCCGGATGATGAATGGCATGAAGCTGAGTGGCCGAGAGATTGACG
  TTCGAATTGATAGAAACGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201584 representing NM_005968
  Red=Cloning site Green=Tags(s)
  
  MAAGVEAAAEVAATEIKMEEESGAPGVPSGNGAPGPKGEGERPAQNEKRKEKNIKRGGNRFEPYANPTKR
  YRAFITNIPFDVKWQSLKDLVKEKVGEVTYVELLMDAEGKSRGCAVVEFKMEESMKKAAEVLNKHSLSGR
  PLKVKEDPDGEHARRAMQKVMATTGGMGMGPGGPGMITIPPSILNNPNIPNEIIHALQAGRLGSTVFVAN
  LDYKVGWKKLKEVFSMAGVVVRADILEDKDGKSRGIGTVTFEQSIEAVQAISMFNGQLLFDRPMHVKMDE
  RALPKGDFFPPERPQQLPHGLGGIGMGLGPGGQPIDANHLNKGIGMGNIGPAGMGMEGIGFGINKMGGME
  GPFGGGMENMGRFGSGMNMGRINEILSNALKRGEIIAKQGGGGGGGSVPGIERMGPGIDRLGGAGMERMG
  AGLGHGMDRVGSEIERMGLVMDRMGSVERMGSGIERMGPLGLDHMASSIERMGQTMERIGSGVERMGAGM
  GFGLERMAAPIDRVGQTIERMGSGVERMGPAIERMGLSMERMVPAGMGAGLERMGPVMDRMATGLERMGA
  NNLERMGLERMGANSLERMGLERMGANSLERMGPAMGPALGAGIERMGLAMGGGGGASFDRAIEMERGNF
  GGSFAGSFGGAGGHAPGVARKACQIFVRNLPFDFTWKMLKDKFNECGHVLYADIKMENGKSKGCGVVKFE
  SPEVAERACRMMNGMKLSGREIDVRIDRNA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005968
• ORF Size: 2190 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005968.5
• RefSeq Size: 2547 bp
• RefSeq ORF: 2193 bp
• Locus ID: 4670
• UniProt ID: P52272
• Cytogenetics: 19p13.2
• Domains: RRM
• Protein Families: Druggable Genome
• Protein Pathways: Spliceosome
• MW: 77.5 kDa
• Gene Summary: This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs. This protein also constitutes a monomer of the N-acetylglucosamine-specific receptor which is postulated to trigger selective recycling of immature GlcNAc-bearing thyroglobulin molecules. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011]