IRF6 (NM_006147) Human Tagged ORF Clone

CAT#: RC201579

IRF6 (Myc-DDK-tagged)-Human interferon regulatory factor 6 (IRF6), transcript variant 1

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY IRF6 (Cat# RC201579, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IRF6(Cat# TA503159). Positive lysates LY401852 (100ug) and LC401852 (20ug) can be purchased separately from OriGene.

• 

  Western blot validation of overexpression lysate (Cat# LY401852) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201579 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified IRF6 protein (Cat# TP301579). The protein was produced from HEK293T cells transfected with IRF6 cDNA clone (Cat# RC201579) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: IRF6
• Synonyms: LPS; OFC6; PIT; PPS; PPS1; VWS; VWS1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201579 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCTCCACCCCCGCAGAGTCCGGCTAAAGCCCTGGCTGGTGGCCCAGGTGGATAGTGGCCTCTACC
  CTGGGCTCATCTGGCTACACAGGGACTCTAAACGCTTCCAGATTCCCTGGAAACATGCCACCCGGCATAG
  CCCTCAACAAGAAGAGGAAAATACCATTTTTAAGGCCTGGGCTGTAGAGACAGGGAAGTACCAGGAAGGG
  GTGGATGACCCTGACCCAGCTAAATGGAAGGCCCAGCTGCGCTGTGCTCTCAATAAGAGCAGAGAATTCA
  ACCTGATGTATGATGGCACCAAGGAGGTGCCCATGAACCCAGTGAAGATATATCAAGTGTGTGACATCCC
  TCAGCCCCAGGGCTCGATCATTAACCCAGGATCCACAGGGTCTGCTCCCTGGGATGAGAAGGATAATGAT
  GTGGATGAAGAAGATGAGGAAGATGAGCTGGATCAGTCGCAGCACCATGTTCCCATCCAGGACACCTTCC
  CCTTCCTGAACATCAATGGTTCTCCCATGGCGCCAGCCAGTGTGGGCAATTGCAGTGTGGGCAACTGCAG
  CCCGGAGGCAGTGTGGCCCAAAACTGAACCCCTGGAGATGGAAGTACCCCAGGCACCTATACAGCCCTTC
  TATAGCTCTCCAGAACTGTGGATCAGCTCTCTCCCAATGACTGACCTGGACATCAAGTTTCAGTACCGTG
  GGAAGGAGTACGGGCAGACCATGACCGTGAGCAACCCTCAGGGCTGCCGACTCTTCTATGGGGACCTGGG
  TCCCATGCCTGACCAGGAGGAGCTCTTTGGTCCCGTCAGCCTGGAGCAGGTCAAATTCCCAGGTCCTGAG
  CATATTACCAATGAGAAGCAGAAGCTGTTCACTAGCAAGCTGCTGGACGTCATGGACAGAGGACTGATCC
  TGGAGGTCAGCGGTCATGCCATTTATGCCATCAGGCTGTGCCAGTGCAAGGTGTACTGGTCTGGGCCATG
  TGCCCCATCACTTGTTGCTCCCAACCTGATTGAGAGACAAAAGAAGGTCAAGCTATTTTGTCTGGAAACA
  TTCCTTAGCGATCTCATTGCCCACCAGAAAGGACAGATAGAGAAGCAGCCACCGTTTGAGATCTACTTAT
  GCTTTGGGGAAGAATGGCCAGATGGGAAACCATTGGAAAGGAAACTCATCTTGGTTCAGGTCATTCCAGT
  AGTGGCTCGGATGATCTACGAGATGTTTTCTGGTGATTTCACACGATCCTTTGATAGTGGCAGTGTCCGC
  CTGCAGATCTCAACCCCAGACATCAAGGATAACATCGTTGCTCAGCTGAAGCAGCTGTACCGCATCCTTC
  AAACCCAGGAGAGCTGGCAGCCCATGCAGCCCACCCCCAGCATGCAACTGCCCCCTGCCCTGCCTCCCCA
  G
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201579 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MALHPRRVRLKPWLVAQVDSGLYPGLIWLHRDSKRFQIPWKHATRHSPQQEEENTIFKAWAVETGKYQEG
  VDDPDPAKWKAQLRCALNKSREFNLMYDGTKEVPMNPVKIYQVCDIPQPQGSIINPGSTGSAPWDEKDND
  VDEEDEEDELDQSQHHVPIQDTFPFLNINGSPMAPASVGNCSVGNCSPEAVWPKTEPLEMEVPQAPIQPF
  YSSPELWISSLPMTDLDIKFQYRGKEYGQTMTVSNPQGCRLFYGDLGPMPDQEELFGPVSLEQVKFPGPE
  HITNEKQKLFTSKLLDVMDRGLILEVSGHAIYAIRLCQCKVYWSGPCAPSLVAPNLIERQKKVKLFCLET
  FLSDLIAHQKGQIEKQPPFEIYLCFGEEWPDGKPLERKLILVQVIPVVARMIYEMFSGDFTRSFDSGSVR
  LQISTPDIKDNIVAQLKQLYRILQTQESWQPMQPTPSMQLPPALPPQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006147
• ORF Size: 1401 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006147.4
• RefSeq Size: 4505 bp
• RefSeq ORF: 1404 bp
• Locus ID: 3664
• UniProt ID: O14896
• Cytogenetics: 1q32.2
• Domains: IRF
• Protein Families: ES Cell Differentiation/IPS, Transcription Factors
• MW: 53.1 kDa
• Gene Summary: This gene encodes a member of the interferon regulatory transcription factor (IRF) family. Family members share a highly-conserved N-terminal helix-turn-helix DNA-binding domain and a less conserved C-terminal protein-binding domain. The encoded protein may be a transcriptional activator. Mutations in this gene can cause van der Woude syndrome and popliteal pterygium syndrome. Mutations in this gene are also associated with non-syndromic orofacial cleft type 6. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2011]