Vimentin (VIM) (NM_003380) Human Tagged ORF Clone
Lenti ORF clone of Human vimentin (VIM), Myc-DDK-tagged
|Type||Human Tagged ORF Clone|
|E. coli Selection||Chloramphenicol (34 ug/mL)|
|Mammalian Cell Selection||Puromycin|
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene|
|ORF Size||1398 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||1847 bp|
|RefSeq ORF||1401 bp|
|Protein Families||ES Cell Differentiation/IPS|
|Gene Summary||This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017]|
|cDNA Clone Resources|
|RC201546||VIM (Myc-DDK-tagged)-Human vimentin (VIM)||
|RC201546L1||Lenti ORF clone of Human vimentin (VIM), Myc-DDK-tagged||
|RC201546L2||Lenti ORF clone of Human vimentin (VIM), mGFP tagged||
|RC201546L4||Lenti ORF clone of Human vimentin (VIM), mGFP tagged||
|RG201546||VIM (GFP-tagged) - Human vimentin (VIM)||
|SC111054||VIM (untagged)-Human vimentin (VIM)||