N myc interactor (NMI) (NM_004688) Human Tagged ORF Clone

CAT#: RC201543

NMI (Myc-DDK-tagged)-Human N-myc (and STAT) interactor (NMI)

Product Images

Circular map for RC201543

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY NMI (Cat# RC201543, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NMI (Cat# TA809982)(1:2000). Positive lysates LY417813 (100ug) and LC417813 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY417813) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201543 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified NMI protein (Cat# TP301543). The protein was produced from HEK293T cells transfected with NMI cDNA clone (Cat# RC201543) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: N myc interactor
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201543 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGCTGATAAAGATGACACACAACAAATTCTTAAGGAGCATTCGCCAGATGAATTTATAAAAGATG
  AACAAAATAAGGGACTAATTGATGAAATTACAAAGAAAAATATTCAACTAAAGAAGGAGATCCAAAAGCT
  TGAAACGGAGTTACAAGAGGCTACCAAAGAATTCCAGATTAAAGAGGATATTCCTGAAACAAAGATGAAA
  TTCTTATCAGTTGAAACTCCTGAGAATGACAGCCAGTTGTCAAATATCTCCTGTTCATTTCAAGTGAGCT
  CGAAAGTTCCTTATGAGATACAAAAAGGACAAGCACTTATCACCTTTGAAAAAGAAGAAGTTGCTCAAAA
  TGTGGTAAGCATGAGTAAACATCATGTACAGATAAAAGATGTAAATCTGGAGGTTACGGCCAAGCCAGTT
  CCATTAAATTCAGGAGTCAGATTCCAGGTTTATGTAGAAGTTTCTAAAATGAAAATCAATGTTACTGAAA
  TTCCTGACACATTGCGTGAAGATCAAATGAGAGACAAACTAGAGCTGAGCTTTTCAAAGTCCCGAAATGG
  AGGCGGAGAGGTGGACCGCGTGGACTATGACAGACAGTCCGGGAGTGCAGTCATCACGTTTGTGGAGATT
  GGAGTGGCTGACAAGATTTTGAAAAAGAAAGAATACCCTCTTTATATAAATCAAACCTGCCATAGAGTTA
  CTGTTTCTCCATACACAGAAATACACTTGAAAAAGTATCAGATATTTTCAGGAACATCTAAGAGGACAGT
  GCTTCTGACAGGAATGGAAGGCATTCAAATGGATGAAGAAATTGTGGAGGATTTAATTAACATTCACTTT
  CAACGGGCAAAGAATGGAGGTGGAGAAGTAGATGTGGTCAAGTGTTCTCTAGGTCAACCTCACATAGCAT
  ACTTTGAAGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201543 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEADKDDTQQILKEHSPDEFIKDEQNKGLIDEITKKNIQLKKEIQKLETELQEATKEFQIKEDIPETKMK
  FLSVETPENDSQLSNISCSFQVSSKVPYEIQKGQALITFEKEEVAQNVVSMSKHHVQIKDVNLEVTAKPV
  PLNSGVRFQVYVEVSKMKINVTEIPDTLREDQMRDKLELSFSKSRNGGGEVDRVDYDRQSGSAVITFVEI
  GVADKILKKKEYPLYINQTCHRVTVSPYTEIHLKKYQIFSGTSKRTVLLTGMEGIQMDEEIVEDLINIHF
  QRAKNGGGEVDVVKCSLGQPHIAYFEE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004688
• ORF Size: 921 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004688.3
• RefSeq Size: 1501 bp
• RefSeq ORF: 924 bp
• Locus ID: 9111
• UniProt ID: Q13287
• Cytogenetics: 2q23.3
• Protein Families: Druggable Genome, Transcription Factors
• MW: 35.1 kDa
• Gene Summary: NMYC interactor (NMI) encodes a protein that interacts with NMYC and CMYC (two members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH-Zip motif. The NMI protein also interacts with all STATs except STAT2 and augments STAT-mediated transcription in response to cytokines IL2 and IFN-gamma. The NMI mRNA has low expression levels in all human fetal and adult tissues tested except brain and has high expression in cancer cell line-myeloid leukemias. [provided by RefSeq, Jul 2008]