TRMT6 (NM_015939) Human Tagged ORF Clone

CAT#: RC201534

TRMT6 (Myc-DDK-tagged)-Human tRNA methyltransferase 6 homolog (S. cerevisiae) (TRMT6)

Product Images

Western blot validation of overexpression lysate (Cat# LY414294) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201534 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified TRMT6 protein (Cat# TP301534). The protein was produced from HEK293T cells transfected with TRMT6 cDNA clone (Cat# RC201534) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TRMT6
• Synonyms: CGI-09; GCD10; Gcd10p
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201534 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGGCTCAGGGGAGCAGCCGGGCCCACAACCACAGCATCCCGGAGACCACCGCATCCGCGACGGCG
  ACTTCGTGGTGCTGAAACGTGAAGATGTGTTTAAAGCAGTACAAGTCCAGCGGAGAAAAAAAGTAACTTT
  CGAAAAACAGTGGTTCTACCTGGATAACGTCATTGGCCATAGTTATGGAACTGCATTTGAAGTGACCAGT
  GGAGGAAGTCTACAGCCCAAGAAGAAGAGGGAAGAGCCTACTGCAGAGACTAAAGAAGCGGGCACTGATA
  ATCGAAATATAGTTGATGATGGGAAATCTCAGAAACTTACTCAAGATGACATAAAAGCTTTGAAGGACAA
  GGGCATTAAAGGAGAGGAAATAGTTCAGCAGTTAATTGAAAATAGTACAACATTCCGAGACAAGACAGAA
  TTTGCCCAAGATAAATATATTAAAAAGAAGAAAAAAAAATATGAAGCCATCATTACTGTTGTGAAGCCAT
  CCACCCGTATTCTTTCAATTATGTATTATGCAAGAGAACCTGGAAAAATTAACCACATGAGATACGATAC
  ACTAGCCCAGATGTTGACGTTGGGAAATATCCGTGCTGGCAACAAAATGATTGTGATGGAAACGTGTGCA
  GGCTTGGTGCTGGGTGCAATGATGGAACGAATGGGAGGTTTTGGCTCCATTATTCAGCTATACCCTGGAG
  GAGGACCTGTTCGGGCAGCAACAGCATGTTTTGGATTTCCCAAATCTTTTCTCAGTGGTCTTTATGAATT
  CCCTCTCAACAAAGTGGACAGTCTTCTACATGGAACATTTTCTGCCAAGATGTTATCTTCAGAGCCAAAA
  GACAGTGCTTTGGTTGAAGAAAGTAATGGCACACTGGAGGAAAAACAGGCTTCTGAACAAGAGAATGAAG
  ACAGCATGGCAGAGGCCCCAGAGAGCAACCACCCAGAAGACCAGGAAACAATGGAAACAATTTCTCAAGA
  TCCAGAACATAAGGGGCCTAAAGAGAGAGGAAGCAAAAAAGATTATATTCAGGAAAAACAGAGGAGACAA
  GAAGAGCAGAGGAAAAGACATTTAGAGGCTGCCGCTCTGCTGAGTGAAAGAAACGCAGATGGTTTAATTG
  TAGCTAGTCGTTTCCACCCCACTCCCCTGCTGCTGTCTTTGCTGGACTTTGTGGCCCCTTCAAGGCCGTT
  TGTGGTCTACTGTCAGTACAAAGAGCCTCTGTTGGAATGCTACACAAAACTGCGGGAGAGGGGAGGGGTC
  ATCAACCTCAGGCTGTCTGAAACCTGGCTCAGAAATTATCAGGTTTTGCCAGATCGAAGTCATCCTAAAC
  TGCTGATGAGTGGAGGTGGGGGTTATCTTCTCTCCGGCTTCACCGTTGCCATGGACAACCTTAAAGCAGA
  CACCAGCCTCAAATCTAATGCAAGCACTTTAGAATCACACGAGACTGAGGAGCCTGCAGCTAAAAAACGA
  AAATGCCCAGAGTCTGACTCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201534 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEGSGEQPGPQPQHPGDHRIRDGDFVVLKREDVFKAVQVQRRKKVTFEKQWFYLDNVIGHSYGTAFEVTS
  GGSLQPKKKREEPTAETKEAGTDNRNIVDDGKSQKLTQDDIKALKDKGIKGEEIVQQLIENSTTFRDKTE
  FAQDKYIKKKKKKYEAIITVVKPSTRILSIMYYAREPGKINHMRYDTLAQMLTLGNIRAGNKMIVMETCA
  GLVLGAMMERMGGFGSIIQLYPGGGPVRAATACFGFPKSFLSGLYEFPLNKVDSLLHGTFSAKMLSSEPK
  DSALVEESNGTLEEKQASEQENEDSMAEAPESNHPEDQETMETISQDPEHKGPKERGSKKDYIQEKQRRQ
  EEQRKRHLEAAALLSERNADGLIVASRFHPTPLLLSLLDFVAPSRPFVVYCQYKEPLLECYTKLRERGGV
  INLRLSETWLRNYQVLPDRSHPKLLMSGGGGYLLSGFTVAMDNLKADTSLKSNASTLESHETEEPAAKKR
  KCPESDS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015939
• ORF Size: 1491 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015939.5
• RefSeq Size: 2353 bp
• RefSeq ORF: 1494 bp
• Locus ID: 51605
• UniProt ID: Q9UJA5
• Cytogenetics: 20p12.3
• MW: 55.8 kDa
• Gene Summary: This gene encodes a member of the tRNA methyltransferase 6 protein family. A similar protein in yeast is part of a two component methyltransferase, which is involved in the posttranslational modification that produces the modified nucleoside 1-methyladenosine in tRNAs. Modified 1-methyladenosine influences initiator methionine stability and may be involved in the replication of human immunodeficiency virus type 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]