TRMT6 (NM_015939) Human Tagged ORF Clone

CAT#: RC201534

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TRMT6 (Myc-DDK-tagged)-Human tRNA methyltransferase 6 homolog (S. cerevisiae) (TRMT6)



Reconstitution Protocol

USD 457.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol TRMT6
Synonyms CGI-09; GCD10; Gcd10p
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC201534 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGGCTCAGGGGAGCAGCCGGGCCCACAACCACAGCATCCCGGAGACCACCGCATCCGCGACGGCG
ACTTCGTGGTGCTGAAACGTGAAGATGTGTTTAAAGCAGTACAAGTCCAGCGGAGAAAAAAAGTAACTTT
CGAAAAACAGTGGTTCTACCTGGATAACGTCATTGGCCATAGTTATGGAACTGCATTTGAAGTGACCAGT
GGAGGAAGTCTACAGCCCAAGAAGAAGAGGGAAGAGCCTACTGCAGAGACTAAAGAAGCGGGCACTGATA
ATCGAAATATAGTTGATGATGGGAAATCTCAGAAACTTACTCAAGATGACATAAAAGCTTTGAAGGACAA
GGGCATTAAAGGAGAGGAAATAGTTCAGCAGTTAATTGAAAATAGTACAACATTCCGAGACAAGACAGAA
TTTGCCCAAGATAAATATATTAAAAAGAAGAAAAAAAAATATGAAGCCATCATTACTGTTGTGAAGCCAT
CCACCCGTATTCTTTCAATTATGTATTATGCAAGAGAACCTGGAAAAATTAACCACATGAGATACGATAC
ACTAGCCCAGATGTTGACGTTGGGAAATATCCGTGCTGGCAACAAAATGATTGTGATGGAAACGTGTGCA
GGCTTGGTGCTGGGTGCAATGATGGAACGAATGGGAGGTTTTGGCTCCATTATTCAGCTATACCCTGGAG
GAGGACCTGTTCGGGCAGCAACAGCATGTTTTGGATTTCCCAAATCTTTTCTCAGTGGTCTTTATGAATT
CCCTCTCAACAAAGTGGACAGTCTTCTACATGGAACATTTTCTGCCAAGATGTTATCTTCAGAGCCAAAA
GACAGTGCTTTGGTTGAAGAAAGTAATGGCACACTGGAGGAAAAACAGGCTTCTGAACAAGAGAATGAAG
ACAGCATGGCAGAGGCCCCAGAGAGCAACCACCCAGAAGACCAGGAAACAATGGAAACAATTTCTCAAGA
TCCAGAACATAAGGGGCCTAAAGAGAGAGGAAGCAAAAAAGATTATATTCAGGAAAAACAGAGGAGACAA
GAAGAGCAGAGGAAAAGACATTTAGAGGCTGCCGCTCTGCTGAGTGAAAGAAACGCAGATGGTTTAATTG
TAGCTAGTCGTTTCCACCCCACTCCCCTGCTGCTGTCTTTGCTGGACTTTGTGGCCCCTTCAAGGCCGTT
TGTGGTCTACTGTCAGTACAAAGAGCCTCTGTTGGAATGCTACACAAAACTGCGGGAGAGGGGAGGGGTC
ATCAACCTCAGGCTGTCTGAAACCTGGCTCAGAAATTATCAGGTTTTGCCAGATCGAAGTCATCCTAAAC
TGCTGATGAGTGGAGGTGGGGGTTATCTTCTCTCCGGCTTCACCGTTGCCATGGACAACCTTAAAGCAGA
CACCAGCCTCAAATCTAATGCAAGCACTTTAGAATCACACGAGACTGAGGAGCCTGCAGCTAAAAAACGA
AAATGCCCAGAGTCTGACTCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC201534 protein sequence
Red=Cloning site Green=Tags(s)

MEGSGEQPGPQPQHPGDHRIRDGDFVVLKREDVFKAVQVQRRKKVTFEKQWFYLDNVIGHSYGTAFEVTS
GGSLQPKKKREEPTAETKEAGTDNRNIVDDGKSQKLTQDDIKALKDKGIKGEEIVQQLIENSTTFRDKTE
FAQDKYIKKKKKKYEAIITVVKPSTRILSIMYYAREPGKINHMRYDTLAQMLTLGNIRAGNKMIVMETCA
GLVLGAMMERMGGFGSIIQLYPGGGPVRAATACFGFPKSFLSGLYEFPLNKVDSLLHGTFSAKMLSSEPK
DSALVEESNGTLEEKQASEQENEDSMAEAPESNHPEDQETMETISQDPEHKGPKERGSKKDYIQEKQRRQ
EEQRKRHLEAAALLSERNADGLIVASRFHPTPLLLSLLDFVAPSRPFVVYCQYKEPLLECYTKLRERGGV
INLRLSETWLRNYQVLPDRSHPKLLMSGGGGYLLSGFTVAMDNLKADTSLKSNASTLESHETEEPAAKKR
KCPESDS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_015939
ORF Size 1491 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_015939.5
RefSeq Size 2353 bp
RefSeq ORF 1494 bp
Locus ID 51605
UniProt ID Q9UJA5
Cytogenetics 20p12.3
MW 55.8 kDa
Gene Summary This gene encodes a member of the tRNA methyltransferase 6 protein family. A similar protein in yeast is part of a two component methyltransferase, which is involved in the posttranslational modification that produces the modified nucleoside 1-methyladenosine in tRNAs. Modified 1-methyladenosine influences initiator methionine stability and may be involved in the replication of human immunodeficiency virus type 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
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