PSF3 (GINS3) (NM_022770) Human Tagged ORF Clone

CAT#: RC201425

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GINS3 (Myc-DDK-tagged)-Human GINS complex subunit 3 (Psf3 homolog) (GINS3), transcript variant 2



  "NM_022770" in other vectors (5)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 118.00

USD 429.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol GINS3
Synonyms PSF3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC201425 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCAGAGGCTTATTTCCGAGTGGAGTCGGGTGCGCTGGGGCCTGAGGAGAACTTTCTTTCTTTGGACG
ACATCCTGATGTCCCACGAGAAGCTGCCGGTGCGCACGGAGACCGCCATGCCTCGCCTTGGCGCTTTCTT
CCTGGAGCGGAGCGCAGGCGCCGAGACTGACAACGCGGTCCCACAGGGTTCCAAGCTTGAACTACCCTTG
TGGCTGGCAAAAGGACTTTTTGACAACAAGCGACGGATCCTTTCTGTGGAACTCCCCAAGATCTACCAAG
AGGGTTGGAGGACTGTGTTCAGTGCAGATCCCAATGTGGTGGACCTCCACAAAATGGGGCCCCATTTCTA
CGGGTTTGGCTCCCAGCTCCTGCATTTTGACAGTCCCGAGAATGCAGACATTTCCCAGTCTCTGCTGCAG
ACTTTTATCGGACGTTTTCGCCGCATCATGGACTCCTCACAGAATGCTTACAACGAAGACACTTCAGCCC
TGGTAGCCAGGCTAGACGAGATGGAGAGGGGCTTATTTCAAACAGGGCAGAAAGGACTGAATGACTTTCA
GTGTTGGGAGAAGGGGCAGGCTTCTCAGATCACAGCTTCCAACCTCGTTCAGAATTACAAGAAGAGAAAA
TTCACTGATATGGAAGAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC201425 protein sequence
Red=Cloning site Green=Tags(s)

MSEAYFRVESGALGPEENFLSLDDILMSHEKLPVRTETAMPRLGAFFLERSAGAETDNAVPQGSKLELPL
WLAKGLFDNKRRILSVELPKIYQEGWRTVFSADPNVVDLHKMGPHFYGFGSQLLHFDSPENADISQSLLQ
TFIGRFRRIMDSSQNAYNEDTSALVARLDEMERGLFQTGQKGLNDFQCWEKGQASQITASNLVQNYKKRK
FTDMED

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_022770
ORF Size 648 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_022770.4
RefSeq Size 2297 bp
RefSeq ORF 651 bp
Locus ID 64785
UniProt ID Q9BRX5
Cytogenetics 16q21
MW 24.5 kDa
Gene Summary This gene encodes a protein subunit of the GINS heterotetrameric complex, which is essential for the initiation of DNA replication and replisome progression in eukaryotes. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

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