EXOSC7 (NM_015004) Human Tagged ORF Clone

CAT#: RC201419

EXOSC7 (Myc-DDK-tagged)-Human exosome component 7 (EXOSC7), transcript variant 1

Product Images

Circular map for RC201419

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY EXOSC7 (Cat# RC201419, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EXOSC7(Cat# TA504239). Positive lysates LY402399 (100ug) and LC402399 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY402399) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201419 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified EXOSC7 protein (Cat# TP301419). The protein was produced from HEK293T cells transfected with EXOSC7 cDNA clone (Cat# RC201419) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EXOSC7
• Synonyms: EAP1; hRrp42p; p8; RRP42; Rrp42p
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201419 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCCGTGACGCTGAGCGAGGCGGAGAAGGTGTACATCGTGCATGGCGTCCAGGAAGACCTCCGTG
  TGGATGGCCGTGGCTGTGAGGACTACCGATGTGTCGAAGTGGAAACTGATGTGGTGTCCAACACTAGTGG
  GTCCGCCAGGGTCAAGCTGGGTCACACAGACATCTTGGTGGGAGTGAAAGCAGAAATGGGGACGCCGAAG
  CTGGAGAAACCAAATGAAGGCTACTTGGAGTTCTTTGTTGACTGTTCAGCCAGTGCTACCCCTGAATTTG
  AAGGTAGAGGAGGTGATGACCTTGGCACCGAGATCGCTAACACCCTCTATCGGATATTTAACAATAAAAG
  CAGTGTCGACTTAAAGACCCTCTGCATTAGTCCTCGGGAGCACTGCTGGGTTCTCTATGTGGATGTGCTG
  CTTCTGGAATGTGGTGGAAATTTGTTTGATGCCATTTCCATTGCTGTAAAGGCTGCTCTCTTCAATACAA
  GGATACCAAGGGTTCGAGTTTTGGAGGATGAAGAGGGGTCGAAGGACATTGAATTGTCAGATGACCCTTA
  TGACTGCATACGACTAAGTGTGGAGAATGTCCCCTGCATTGTCACTCTGTGCAAGATTGGCTATCGGCAT
  GTGGTGGATGCTACTCTTCAGGAGGAGGCCTGCTCGCTGGCCAGCTTGCTGGTGTCGGTGACCAGCAAGG
  GAGTTGTGACGTGCATGAGGAAAGTGGGGAAGGGCAGCCTGGACCCAGAGAGCATCTTCGAGATGATGGA
  GACTGGCAAGCGTGTGGGCAAGGTACTGCATGCCTCCTTGCAGAGTGTTCTGCACAAGGAAGAAAGCCTG
  GGGCCCAAGAGACAGAAAGTTGGATTCCTGGGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201419 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MASVTLSEAEKVYIVHGVQEDLRVDGRGCEDYRCVEVETDVVSNTSGSARVKLGHTDILVGVKAEMGTPK
  LEKPNEGYLEFFVDCSASATPEFEGRGGDDLGTEIANTLYRIFNNKSSVDLKTLCISPREHCWVLYVDVL
  LLECGGNLFDAISIAVKAALFNTRIPRVRVLEDEEGSKDIELSDDPYDCIRLSVENVPCIVTLCKIGYRH
  VVDATLQEEACSLASLLVSVTSKGVVTCMRKVGKGSLDPESIFEMMETGKRVGKVLHASLQSVLHKEESL
  GPKRQKVGFLG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015004
• ORF Size: 873 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015004.2, NP_055819.1
• RefSeq Size: 1059 bp
• RefSeq ORF: 876 bp
• Locus ID: 23016
• UniProt ID: Q15024
• Cytogenetics: 3p21.31
• Domains: RNase_PH_C
• Protein Families: Stem cell - Pluripotency
• Protein Pathways: RNA degradation
• MW: 31.8 kDa
• Gene Summary: Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes.[UniProtKB/Swiss-Prot Function]