SESN2 (NM_031459) Human Tagged ORF Clone

CAT#: RC201386

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SESN2 (Myc-DDK-tagged)-Human sestrin 2 (SESN2)



  "NM_031459" in other vectors (7)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 477.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol SESN2
Synonyms HI95; SES2; SEST2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC201386 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATCGTGGCGGACTCCGAGTGCCGCGCAGAGCTCAAGGACTACCTGCGGTTCGCCCCGGGCGGCGTCG
GCGACTCGGGCCCCGGAGAGGAGCAGAGGGAGAGCCGGGCTCGGCGAGGCCCTCGAGGGCCCAGCGCCTT
CATCCCCGTGGAGGAGGTCCTTCGGGAGGGGGCTGAGAGCCTCGAGCAGCACCTGGGGCTGGAGGCACTG
ATGTCCTCTGGGCGAGTAGACAACCTGGCAGTGGTGATGGGCCTGCACCCTGACTACTTTACCAGCTTCT
GGCGCCTGCACTACCTGCTGCTGCACACGGATGGTCCCTTGGCCAGCTCCTGGCGCCACTACATTGCCAT
CATGGCTGCCGCCCGCCATCAGTGTTCTTACCTGGTAGGCTCCCACATGGCCGAGTTTCTGCAGACTGGT
GGTGACCCTGAGTGGCTGCTGGGCCTCCACCGGGCCCCCGAGAAGCTGCGCAAACTCAGCGAGATCAACA
AGTTGCTGGCGCATCGGCCATGGCTCATCACCAAGGAACACATCCAGGCCTTGCTGAAGACCGGCGAGCA
CACTTGGTCCCTGGCCGAGCTCATTCAGGCTCTGGTCCTGCTCACCCACTGCCACTCGCTCTCCTCCTTC
GTGTTTGGCTGTGGCATCCTCCCTGAGGGGGATGCAGATGGCAGCCCTGCCCCCCAGGCACCTACACCCC
CTAGTGAACAGAGCAGCCCCCCAAGCAGGGACCCGTTGAACAACTCTGGGGGCTTTGAGTCTGCCCGCGA
CGTGGAGGCGCTGATGGAGCGCATGCAGCAGCTGCAGGAGAGCCTGCTGCGGGATGAGGGGACGTCCCAG
GAGGAGATGGAGAGCCGCTTTGAGCTGGAGAAGTCAGAGAGCCTGCTGGTGACCCCCTCAGCTGACATCC
TGGAGCCCTCTCCACACCCAGACATGCTGTGCTTTGTGGAAGACCCTACTTTCGGATATGAGGACTTCAC
TCGGAGAGGGGCTCAGGCACCCCCTACCTTCCGGGCCCAGGATTATACCTGGGAAGACCATGGCTACTCG
CTGATCCAGCGGCTTTACCCTGAGGGTGGGCAGCTGCTGGATGAGAAGTTCCAGGCAGCCTATAGCCTCA
CCTACAATACCATCGCCATGCACAGTGGTGTGGACACCTCCGTGCTCCGCAGGGCCATCTGGAACTATAT
CCACTGCGTCTTTGGCATCAGATATGATGACTATGATTATGGGGAGGTGAACCAGCTCCTGGAGCGGAAC
CTCAAGGTCTATATCAAGACAGTGGCCTGCTACCCAGAGAAGACCACCCGAAGAATGTACAACCTCTTCT
GGAGGCACTTCCGCCACTCAGAGAAGGTCCACGTGAACTTGCTGCTCCTGGAGGCGCGCATGCAAGCCGC
TCTGCTGTACGCCCTCCGTGCCATCACCCGCTACATGACC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC201386 protein sequence
Red=Cloning site Green=Tags(s)

MIVADSECRAELKDYLRFAPGGVGDSGPGEEQRESRARRGPRGPSAFIPVEEVLREGAESLEQHLGLEAL
MSSGRVDNLAVVMGLHPDYFTSFWRLHYLLLHTDGPLASSWRHYIAIMAAARHQCSYLVGSHMAEFLQTG
GDPEWLLGLHRAPEKLRKLSEINKLLAHRPWLITKEHIQALLKTGEHTWSLAELIQALVLLTHCHSLSSF
VFGCGILPEGDADGSPAPQAPTPPSEQSSPPSRDPLNNSGGFESARDVEALMERMQQLQESLLRDEGTSQ
EEMESRFELEKSESLLVTPSADILEPSPHPDMLCFVEDPTFGYEDFTRRGAQAPPTFRAQDYTWEDHGYS
LIQRLYPEGGQLLDEKFQAAYSLTYNTIAMHSGVDTSVLRRAIWNYIHCVFGIRYDDYDYGEVNQLLERN
LKVYIKTVACYPEKTTRRMYNLFWRHFRHSEKVHVNLLLLEARMQAALLYALRAITRYMT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_031459
ORF Size 1440 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_031459.5
RefSeq Size 3547 bp
RefSeq ORF 1443 bp
Locus ID 83667
UniProt ID P58004
Cytogenetics 1p35.3
Domains PA26
Protein Pathways p53 signaling pathway
MW 54.5 kDa
Gene Summary This gene encodes a member of the sestrin family of PA26-related proteins. The encoded protein may function in the regulation of cell growth and survival. This protein may be involved in cellular response to different stress conditions. [provided by RefSeq, Jul 2008]

Other Versions

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