SESN2 (NM_031459) Human Tagged ORF Clone

CAT#: RC201386

SESN2 (Myc-DDK-tagged)-Human sestrin 2 (SESN2)

Product Images

Circular map for RC201386

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  Western blot validation of overexpression lysate (Cat# LY403117) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201386 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified SESN2 protein (Cat# TP301386). The protein was produced from HEK293T cells transfected with SESN2 cDNA clone (Cat# RC201386) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SESN2
• Synonyms: HI95; SES2; SEST2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201386 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCGTGGCGGACTCCGAGTGCCGCGCAGAGCTCAAGGACTACCTGCGGTTCGCCCCGGGCGGCGTCG
  GCGACTCGGGCCCCGGAGAGGAGCAGAGGGAGAGCCGGGCTCGGCGAGGCCCTCGAGGGCCCAGCGCCTT
  CATCCCCGTGGAGGAGGTCCTTCGGGAGGGGGCTGAGAGCCTCGAGCAGCACCTGGGGCTGGAGGCACTG
  ATGTCCTCTGGGCGAGTAGACAACCTGGCAGTGGTGATGGGCCTGCACCCTGACTACTTTACCAGCTTCT
  GGCGCCTGCACTACCTGCTGCTGCACACGGATGGTCCCTTGGCCAGCTCCTGGCGCCACTACATTGCCAT
  CATGGCTGCCGCCCGCCATCAGTGTTCTTACCTGGTAGGCTCCCACATGGCCGAGTTTCTGCAGACTGGT
  GGTGACCCTGAGTGGCTGCTGGGCCTCCACCGGGCCCCCGAGAAGCTGCGCAAACTCAGCGAGATCAACA
  AGTTGCTGGCGCATCGGCCATGGCTCATCACCAAGGAACACATCCAGGCCTTGCTGAAGACCGGCGAGCA
  CACTTGGTCCCTGGCCGAGCTCATTCAGGCTCTGGTCCTGCTCACCCACTGCCACTCGCTCTCCTCCTTC
  GTGTTTGGCTGTGGCATCCTCCCTGAGGGGGATGCAGATGGCAGCCCTGCCCCCCAGGCACCTACACCCC
  CTAGTGAACAGAGCAGCCCCCCAAGCAGGGACCCGTTGAACAACTCTGGGGGCTTTGAGTCTGCCCGCGA
  CGTGGAGGCGCTGATGGAGCGCATGCAGCAGCTGCAGGAGAGCCTGCTGCGGGATGAGGGGACGTCCCAG
  GAGGAGATGGAGAGCCGCTTTGAGCTGGAGAAGTCAGAGAGCCTGCTGGTGACCCCCTCAGCTGACATCC
  TGGAGCCCTCTCCACACCCAGACATGCTGTGCTTTGTGGAAGACCCTACTTTCGGATATGAGGACTTCAC
  TCGGAGAGGGGCTCAGGCACCCCCTACCTTCCGGGCCCAGGATTATACCTGGGAAGACCATGGCTACTCG
  CTGATCCAGCGGCTTTACCCTGAGGGTGGGCAGCTGCTGGATGAGAAGTTCCAGGCAGCCTATAGCCTCA
  CCTACAATACCATCGCCATGCACAGTGGTGTGGACACCTCCGTGCTCCGCAGGGCCATCTGGAACTATAT
  CCACTGCGTCTTTGGCATCAGATATGATGACTATGATTATGGGGAGGTGAACCAGCTCCTGGAGCGGAAC
  CTCAAGGTCTATATCAAGACAGTGGCCTGCTACCCAGAGAAGACCACCCGAAGAATGTACAACCTCTTCT
  GGAGGCACTTCCGCCACTCAGAGAAGGTCCACGTGAACTTGCTGCTCCTGGAGGCGCGCATGCAAGCCGC
  TCTGCTGTACGCCCTCCGTGCCATCACCCGCTACATGACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201386 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MIVADSECRAELKDYLRFAPGGVGDSGPGEEQRESRARRGPRGPSAFIPVEEVLREGAESLEQHLGLEAL
  MSSGRVDNLAVVMGLHPDYFTSFWRLHYLLLHTDGPLASSWRHYIAIMAAARHQCSYLVGSHMAEFLQTG
  GDPEWLLGLHRAPEKLRKLSEINKLLAHRPWLITKEHIQALLKTGEHTWSLAELIQALVLLTHCHSLSSF
  VFGCGILPEGDADGSPAPQAPTPPSEQSSPPSRDPLNNSGGFESARDVEALMERMQQLQESLLRDEGTSQ
  EEMESRFELEKSESLLVTPSADILEPSPHPDMLCFVEDPTFGYEDFTRRGAQAPPTFRAQDYTWEDHGYS
  LIQRLYPEGGQLLDEKFQAAYSLTYNTIAMHSGVDTSVLRRAIWNYIHCVFGIRYDDYDYGEVNQLLERN
  LKVYIKTVACYPEKTTRRMYNLFWRHFRHSEKVHVNLLLLEARMQAALLYALRAITRYMT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031459
• ORF Size: 1440 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031459.5
• RefSeq Size: 3547 bp
• RefSeq ORF: 1443 bp
• Locus ID: 83667
• UniProt ID: P58004
• Cytogenetics: 1p35.3
• Domains: PA26
• Protein Pathways: p53 signaling pathway
• MW: 54.5 kDa
• Gene Summary: This gene encodes a member of the sestrin family of PA26-related proteins. The encoded protein may function in the regulation of cell growth and survival. This protein may be involved in cellular response to different stress conditions. [provided by RefSeq, Jul 2008]