UBAP2L (NM_014847) Human Tagged ORF Clone

CAT#: RC201371

UBAP2L (Myc-DDK-tagged)-Human ubiquitin associated protein 2-like (UBAP2L), transcript variant 1

Product Images

Circular map for RC201371

• 

  Western blot validation of overexpression lysate (Cat# LY414971) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201371 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified UBAP2L protein (Cat# TP301371). The protein was produced from HEK293T cells transfected with UBAP2L cDNA clone (Cat# RC201371) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UBAP2L
• Synonyms: NICE-4; NICE4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201371 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGACATCGGTGGGCACTAACCGAGCCCGGGGAAACTGGGAACAACCTCAAAACCAAAACCAGACAC
  AGCACAAGCAGCGGCCACAGGCCACTGCAGAACAAATTAGACTTGCACAGATGATTTCGGACCATAATGA
  TGCTGACTTTGAGGAGAAGGTGAAACAATTGATTGATATTACAGGCAAGAACCAGGATGAATGTGTGATT
  GCTTTGCATGACTGCAATGGAGATGTCAACAGAGCTATCAATGTTCTTCTGGAAGGAAACCCAGACACGC
  ATTCCTGGGAGATGGTCGGGAAGAAGAAGGGAGTCTCAGGCCAGAAGGATGGTGGCCAGACGGAATCCAA
  TGAGGAAGGCAAAGAAAATCGAGACCGGGACAGAGACTATAGTCGGCGACGTGGTGGGCCACCAAGACGG
  GGGAGAGGTGCCAGCCGTGGACGAGAGTTTCGAGGTCAGGAAAATGGATTGGATGGCACCAAGAGTGGAG
  GGCCTTCTGGAAGAGGAACAGAAAGAGGCAGAAGGGGCCGTGGCCGAGGCAGAGGTGGCTCTGGTAGGCG
  AGGAGGAAGGTTTTCTGCTCAAGGAATGGGAACCTTTAACCCAGCTGATTATGCAGAGCCAGCCAATACT
  GATGATAACTATGGCAATAGCAGCGGCAATACGTGGAACAACACTGGCCACTTTGAACCAGATGATGGGA
  CGAGTGCATGGAGGACTGCAACAGAGGAGTGGGGGACTGAAGATTGGAATGAAGATCTTTCTGAGACCAA
  GATCTTCACTGCCTCTAATGTGTCTTCAGTGCCTCTGCCTGCGGAGAATGTGACAATCACTGCTGGTCAG
  AGAATTGACCTTGCTGTTCTGCTGGGGAAGACACCATCTACAATGGAGAATGATTCATCTAATCTGGATC
  CGTCTCAGGCTCCTTCTCTGGCCCAGCCTCTGGTGTTCAGTAATTCGAAGCAGACTGCCATATCACAGCC
  TGCTTCAGGGAACACATTTTCTCATCACAGTATGGTGAGCATGTTAGGGAAAGGATTTGGTGATGTCGGT
  GAAGCTAAAGGCGGCAGTACTACAGGCTCCCAGTTCTTGGAGCAATTCAAGACTGCCCAAGCCCTGGCTC
  AGTTGGCAGCTCAGCATTCTCAGTCTGGAAGCACCACCACCTCCTCTTGGGACATGGGCTCGACGACACA
  ATCCCCATCACTGGTGCAGTATGATTTGAAGAACCCAAGTGATTCAGCAGTGCACAGCCCCTTTACAAAG
  CGCCAGGCTTTTACCCCATCTTCAACCATGATGGAGGTGTTCCTTCAGGAGAAGTCACCTGCAGTGGCTA
  CCTCCACAGCTGCACCTCCACCTCCGTCTTCTCCTCTGCCAAGCAAATCCACATCGGCTCCACAGATGTC
  GCCTGGATCTTCAGACAACCAGTCCTCTAGCCCTCAGCCGGCTCACCAGAAACTGAAACAGCAGAAGAAA
  AAAGCCTCCTTGACTTCTAAGATTCCTGCTCTGGCTGTGGAGATGCCTGGCTCAGCAGATATCTCAGGGC
  TAAACCTGCAGTTTGGGGCATTGCAGTTTGGGTCAGAGCCTGTCCTTTCTGATTATGAGTCCACCCCCAC
  CACGAGCGCCTCTTCAAGCCAGGCTCCAAGTAGCCTGTATACCAGCACGGCCAGTGAATCATCCTCTACA
  ATTTCATCTAACCAGAGTCAGGAGTCTGGTTATCAGAGCGGCCCAATTCAGTCGACAACCTATACCTCCC
  AAAATAATGCTCAGGGCCCTCTTTATGAACAGAGATCCACACAGACTCGGCGGTACCCCAGCTCCATCTC
  TTCATCACCCCAAAAGGACCTGACTCAGGCAAAGAATGGCTTCAGTTCTGTGCAGGCCACGCAGTTACAG
  ACCACACAATCTGTTGAAGGTGCTACAGGCTCTGCAGTGAAATCTGATTCACCTTCCACTTCTAGCATCC
  CCCCTCTCAATGAAACGGTATCTGCAGCTTCCTTACTGACGACAACCAATCAGCATTCATCCTCCTTGGG
  TGGCTTGAGCCACAGTGAGGAGATTCCAAATACTACCACCACACAACACAGCAGCACGTTATCTACGCAG
  CAGAATACCCTTTCATCATCAACATCTTCTGGGCGCACTTCGACATCCACTCTTTTGCACACAAGTGTGG
  AGAGTGAGGCGAATCTCCATTCTTCCTCCAGCACTTTTTCCACCACATCCAGCACAGTCTCTGCACCTCC
  CCCAGTGGTCAGTGTCTCCTCCAGTCTCAATAGTGGCAGTAGCCTGGGCCTCAGCCTAGGCAGCAACTCC
  ACTGTCACAGCCTCGACTCGAAGCTCAGTTGCTACGACTTCAGGAAAAGCTCCTCCCAACCTCCCTCCTG
  GGGTCCCGCCGTTGTTGCCTAATCCGTATATTATGGCTCCAGGGCTGTTACATGCCTACCCGCCACAAGT
  ATATGGTTATGATGACTTGCAGATGCTTCAGACAAGATTTCCATTGGATTACTACAGCATCCCATTTCCC
  ACACCCACTACTCCGCTGACTGGGAGGGATGGTAGCCTGGCCAGCAACCCTTATTCTGGTGACCTCACAA
  AGTTCGGCCGTGGGGATGCCTCCTCCCCAGCCCCGGCCACAACCTTGGCCCAACCCCAACAGAACCAGAC
  GCAGACTCACCATACCACGCAGCAGACATTCCTGAACCCGGCGCTGCCTCCTGGCTACAGTTACACCAGC
  CTGCCATACTATACAGGGGTCCCGGGCCTCCCCAGCACCTTCCAGTATGGGCCTGCTGTGTTCCCTGTGG
  CTCCTACCTCTTCCAAGCAGCATGGTGTGAATGTCAGTGTGAATGCATCGGCCACCCCTTTCCAACAGCC
  GAGTGGATATGGGTCTCATGGATACAACACTGGTGTTTCAGTCACCTCCAGTAACACGGGCGTGCCAGAT
  ATCTCGGGTTCTGTGTACTCCAAAACCCAGCAGTCCTTTGAGAAACAAGGTTTTCATTCCGGTACTCCTG
  CTGCTTCCTTCAACTTGCCTTCAGCCCTAGGAAGTGGGGGCCCCATCAATCCGGCCACAGCTGCTGCCTA
  CCCACCTGCCCCCTTTATGCACATTCTGACCCCCCATCAGCAGCCGCATTCTCAGATCCTTCACCATCAC
  CTGCAGCAGGATGGCCAGACGGGCAGCGGGCAACGTAGCCAGACCAGCTCCATCCCGCAGAAGCCCCAGA
  CCAACAAGTCTGCCTACAACAGCTACAGCTGGGGGGCCAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201371 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MMTSVGTNRARGNWEQPQNQNQTQHKQRPQATAEQIRLAQMISDHNDADFEEKVKQLIDITGKNQDECVI
  ALHDCNGDVNRAINVLLEGNPDTHSWEMVGKKKGVSGQKDGGQTESNEEGKENRDRDRDYSRRRGGPPRR
  GRGASRGREFRGQENGLDGTKSGGPSGRGTERGRRGRGRGRGGSGRRGGRFSAQGMGTFNPADYAEPANT
  DDNYGNSSGNTWNNTGHFEPDDGTSAWRTATEEWGTEDWNEDLSETKIFTASNVSSVPLPAENVTITAGQ
  RIDLAVLLGKTPSTMENDSSNLDPSQAPSLAQPLVFSNSKQTAISQPASGNTFSHHSMVSMLGKGFGDVG
  EAKGGSTTGSQFLEQFKTAQALAQLAAQHSQSGSTTTSSWDMGSTTQSPSLVQYDLKNPSDSAVHSPFTK
  RQAFTPSSTMMEVFLQEKSPAVATSTAAPPPPSSPLPSKSTSAPQMSPGSSDNQSSSPQPAHQKLKQQKK
  KASLTSKIPALAVEMPGSADISGLNLQFGALQFGSEPVLSDYESTPTTSASSSQAPSSLYTSTASESSST
  ISSNQSQESGYQSGPIQSTTYTSQNNAQGPLYEQRSTQTRRYPSSISSSPQKDLTQAKNGFSSVQATQLQ
  TTQSVEGATGSAVKSDSPSTSSIPPLNETVSAASLLTTTNQHSSSLGGLSHSEEIPNTTTTQHSSTLSTQ
  QNTLSSSTSSGRTSTSTLLHTSVESEANLHSSSSTFSTTSSTVSAPPPVVSVSSSLNSGSSLGLSLGSNS
  TVTASTRSSVATTSGKAPPNLPPGVPPLLPNPYIMAPGLLHAYPPQVYGYDDLQMLQTRFPLDYYSIPFP
  TPTTPLTGRDGSLASNPYSGDLTKFGRGDASSPAPATTLAQPQQNQTQTHHTTQQTFLNPALPPGYSYTS
  LPYYTGVPGLPSTFQYGPAVFPVAPTSSKQHGVNVSVNASATPFQQPSGYGSHGYNTGVSVTSSNTGVPD
  ISGSVYSKTQQSFEKQGFHSGTPAASFNLPSALGSGGPINPATAAAYPPAPFMHILTPHQQPHSQILHHH
  LQQDGQTGSGQRSQTSSIPQKPQTNKSAYNSYSWGAN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014847
• ORF Size: 3261 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014847.2, NP_055662.2
• RefSeq Size: 4006 bp
• RefSeq ORF: 3264 bp
• Locus ID: 9898
• UniProt ID: Q14157
• Cytogenetics: 1q21.3
• Domains: UBA
• Protein Families: Druggable Genome
• MW: 114.5 kDa
• Gene Summary: Plays an important role in the activity of long-term repopulating hematopoietic stem cells (LT-HSCs).[UniProtKB/Swiss-Prot Function]