IST1 (NM_014761) Human Tagged ORF Clone

CAT#: RC201347

IST1 (Myc-DDK-tagged)-Human KIAA0174 (KIAA0174)

Product Images

Circular map for RC201347

• 

  Western blot validation of overexpression lysate (Cat# LY402372) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201347 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: IST1
• Synonyms: CHMP8; OLC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201347 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGGCTCTGGATTTAAAGCTGAGCGCTTAAGAGTGAATTTGAGATTAGTCATAAATCGCCTTAAAC
  TATTGGAGAAAAAGAAAACGGAACTGGCCCAGAAAGCAAGGAAGGAGATTGCTGACTATCTGGCTGCTGG
  GAAAGATGAACGAGCTCGGATCCGTGTGGAGCACATTATCCGGGAAGACTACCTCGTGGAGGCCATGGAG
  ATCCTGGAGCTGTACTGTGACCTGCTGCTGGCTCGGTTTGGCCTTATCCAGTCTATGAAGGAACTAGATT
  CTGGTCTGGCTGAATCTGTGTCTACATTGATCTGGGCTGCTCCTCGACTCCAGTCAGAAGTGGCTGAGTT
  GAAAATAGTTGCTGATCAGCTCTGTGCCAAGTATAGCAAGGAATATGGCAAGCTATGTAGGACCAACCAG
  ATTGGAACTGTGAATGACAGGCTAATGCACAAGCTGAGTGTGGAAGCCCCACCCAAAATCCTGGTGGAGA
  GATACCTGATTGAAATTGCAAAGAATTACAACGTACCCTATGAACCTGACTCTGTGGTCATGGCAGAAGC
  TCCTCCTGGGGTAGAGACAGATCTTATTGATGTTGGATTCACAGATGATGTGAAGAAAGGAGGCCCTGGA
  AGAGGAGGGAGTGGTGGCTTCACAGCACCAGTTGGTGGACCTGATGGAACGGTGCCAATGCCCATGCCCA
  TGCCCATGCCTATGCCATCTGCAAATACGCCTTTCTCATATCCACTGCCAAAGGGACCATCAGATTTCAA
  TGGACTGCCAATGGGGACTTATCAGGCCTTTCCCAATATTCATCCACCTCAGATACCAGCAACTCCCCCA
  TCGTATGAATCTATGACATTAATGCTGATAAGAATATCTCTTCTGCACAGATTGTTGGTCCTGGACCCAA
  GCCAGAAGCCTCTGCAAAGCTTCCTTCCAGACCTGCAGATAACTATGACAACTTTGTCCTACCAGAGTTG
  CCATCTGTGCCAGACACACTACCAACTGCATCTGCTGGTGCCAGCACCTCAGCATCTGAAGACATTGACT
  TTGATGATCTTTCCCGGAGGTTTGAAGAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201347 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLVEAME
  ILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCAKYSKEYGKLCRTNQ
  IGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEPDSVVMAEAPPGVETDLIDVGFTDDVKKGGPG
  RGGSGGFTAPVGGPDGTVPMPMPMPMPMPSANTPFSYPLPKGPSDFNGLPMGTYQAFPNIHPPQIPATPP
  SYESMTLMLIRISLLHRLLVLDPSQKPLQSFLPDLQITMTTLSYQSCHLCQTHYQLHLLVPAPQHLKTLT
  LMIFPGGLKS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014761
• ORF Size: 1080 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014761.4
• RefSeq Size: 4035 bp
• RefSeq ORF: 1083 bp
• Locus ID: 9798
• UniProt ID: P53990
• Cytogenetics: 16q22.2
• Domains: DUF292
• MW: 39.9 kDa
• Gene Summary: This gene encodes a protein with MIT-interacting motifs that interacts with components of endosomal sorting complexes required for transport (ESCRT). ESCRT functions in vesicle budding, such as that which occurs during membrane abscission in cytokinesis. There is a pseudogene for this gene on chromosome 19. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012]