TNIP2 (NM_024309) Human Tagged ORF Clone

CAT#: RC201339

TNIP2 (Myc-DDK-tagged)-Human TNFAIP3 interacting protein 2 (TNIP2), transcript variant 1

Product images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY TNIP2 (Cat# RC201339, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TNIP2(Cat# TA590455). Positive lysates LY411328 (100ug) and LC411328 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY411328) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201339 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified TNIP2 protein (Cat# TP301339). The protein was produced from HEK293T cells transfected with TNIP2 cDNA clone (Cat# RC201339) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TNIP2
• Synonyms: ABIN2; FLIP1; KLIP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201339 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCGGGACCCGGGGTCGGGCGGCTGGGAGGAGGCCCCGCGCGCAGCTGCCGCGCTCTGCACCCTGT
  ACCACGAGGCCGGACAGCGGCTGCGCCGCCTGCAGGACCAGCTCGCTGCCCGCGACGCCCTCATCGCTCG
  CCTCCGCGCCCGCCTGGCCGCGCTGGAGGGGGACGCCGCGCCGTCCCTAGTGGACGCGCTGCTGGAGCAG
  GTTGCGCGCTTCCGGGAGCAGCTGCGAAGGCAGGAGGGCGGCGCCGCCGAGGCCCAGATGCGCCAGGAAA
  TTGAGAGGCTGACTGAGCGACTAGAAGAAAAAGAGAGGGAGATGCAGCAGCTGCTGAGCCAGCCCCAACA
  CGAGCGAGAGAAGGAAGTCGTCCTGCTACGGAGGAGCATGGCAGAAGGGGAGCGCGCCCGGGCCGCCAGT
  GACGTCCTGTGCCGCTCCTTGGCCAACGAGACCCATCAGCTGCGGAGGACGCTGACCGCCACCGCCCACA
  TGTGTCAGCATCTGGCCAAGTGTCTGGATGAACGACAGCATGCACAAAGGAATGTGGGGGAGAGAAGTCC
  TGACCAGTCGGAACACACAGATGGGCACACCTCTGTCCAGAGTGTTATTGAGAAGTTGCAGGAAGAAAAT
  CGACTGTTAAAACAGAAGGTGACTCACGTTGAAGACCTCAATGCCAAGTGGCAGCGCTACAACGCCAGCA
  GGGACGAATACGTGAGGGGGCTCCATGCGCAGCTCAGGGGGCTGCAGATCCCCCACGAGCCCGAGCTGAT
  GAGGAAGGAGATCTCCCGGCTCAACAGACAGTTGGAAGAGAAAATAAATGACTGTGCCGAAGTGAAGCAG
  GAGCTGGCGGCCTCCAGGACGGCCCGGGATGCTGCGTTGGAGCGGGTGCAGATGCTGGAACAGCAGATTC
  TCGCTTACAAGGATGACTTCATGTCAGAAAGGGCCGATCGGGAACGGGCTCAAAGTAGGATTCAAGAACT
  GGAGGAAAAGGTCGCCTCTTTGCTGCACCAGGTGTCCTGGAGACAGGATTCTCGAGAGCCAGACGCCGGC
  CGGATTCACGCTGGGAGCAAAACTGCCAAGTATTTGGCCGCCGACGCATTAGAGCTTATGGTGCCTGGTG
  GCTGGAGGCCTGGGACTGGGTCCCAGCAGCCAGAACCCCCTGCAGAGGGCGGGCATCCTGGCGCGGTCCA
  GAGAGGCCAGGGGGACCTTCAGTGCCCTCACTGCCTGCAGTGCTTCAGTGACGAGCAAGGGGAAGAGCTC
  CTCAGGCATGTGGCCGAGTGCTGCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201339 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSRDPGSGGWEEAPRAAAALCTLYHEAGQRLRRLQDQLAARDALIARLRARLAALEGDAAPSLVDALLEQ
  VARFREQLRRQEGGAAEAQMRQEIERLTERLEEKEREMQQLLSQPQHEREKEVVLLRRSMAEGERARAAS
  DVLCRSLANETHQLRRTLTATAHMCQHLAKCLDERQHAQRNVGERSPDQSEHTDGHTSVQSVIEKLQEEN
  RLLKQKVTHVEDLNAKWQRYNASRDEYVRGLHAQLRGLQIPHEPELMRKEISRLNRQLEEKINDCAEVKQ
  ELAASRTARDAALERVQMLEQQILAYKDDFMSERADRERAQSRIQELEEKVASLLHQVSWRQDSREPDAG
  RIHAGSKTAKYLAADALELMVPGGWRPGTGSQQPEPPAEGGHPGAVQRGQGDLQCPHCLQCFSDEQGEEL
  LRHVAECCQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024309
• ORF Size: 1287 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024309.2, NP_077285.2
• RefSeq Size: 1993 bp
• RefSeq ORF: 1290 bp
• Locus ID: 79155
• UniProt ID: Q8NFZ5
• Cytogenetics: 4p16.3
• MW: 48.7 kDa
• Gene Summary: This gene encodes a protein which acts as an inhibitor of NFkappaB activation. The encoded protein is also involved in MAP/ERK signaling pathway in specific cell types. It may be involved in apoptosis of endothelial cells. Alternative splicing results in multiple transcript variants. A pseudogene related to this gene is located on the X chromosome.[provided by RefSeq, May 2014]