NR2F6 (NM_005234) Human Tagged ORF Clone
NR2F6 (Myc-DDK-tagged)-Human nuclear receptor subfamily 2, group F, member 6 (NR2F6)
|Product Name||NR2F6 (NM_005234) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|Synonyms||EAR-2; EAR2; ERBAL2|
|E. coli Selection||Kanamycin (25 ug/mL)|
>RC201336 representing NM_005234
Red=Cloning site Blue=ORF Green=Tags(s)
>RC201336 representing NM_005234
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||1212 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at firstname.lastname@example.org or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||1804 bp|
|RefSeq ORF||1215 bp|
|Protein Families||Druggable Genome, Nuclear Hormone Receptor, Transcription Factors|
|Gene Summary||Transcription factor predominantly involved in transcriptional repression. Binds to promoter/enhancer response elements that contain the imperfect 5'-AGGTCA-3' direct or inverted repeats with various spacings which are also recognized by other nuclear hormone receptors. Involved in modulation of hormonal responses. Represses transcriptional activity of the lutropin-choriogonadotropic hormone receptor/LHCGR gene, the renin/REN gene and the oxytocin-neurophysin/OXT gene. Represses the triiodothyronine-dependent and -independent transcriptional activity of the thyroid hormone receptor gene in a cell type-specific manner. The corepressing function towards thyroid hormone receptor beta/THRB involves at least in part the inhibition of THRB binding to triiodothyronine response elements (TREs) by NR2F6. Inhibits NFATC transcription factor DNA binding and subsequently its transcriptional activity. Acts as transcriptional repressor of IL-17 expression in Th-17 differentiated CD4(+) T cells and may be involved in induction and/or maintenance of peripheral immunological tolerance and autoimmunity. Involved in development of forebrain circadian clock; is required early in the development of the locus coeruleus (LC). [UniProtKB/Swiss-Prot Function]|
|The use of this cDNA Clones has been cited in the following citations:|
DDA1 is induced by NR2F6 in ovarian cancer and predicts poor survival outcome
,Liu, J;Li, T;Liu, XL;,
Eur Rev Med Pharmacol Sci 2017
,PubMed ID 28387912
|cDNA Clone Resources|
|SC116862||NR2F6 (untagged)-Human nuclear receptor subfamily 2, group F, member 6 (NR2F6)||
|RG201336||NR2F6 (GFP-tagged) - Human nuclear receptor subfamily 2, group F, member 6 (NR2F6)||
|RC201336L1||Lenti ORF clone of Human nuclear receptor subfamily 2, group F, member 6 (NR2F6), Myc-DDK-tagged||
|RC201336L2||Lenti ORF clone of Human nuclear receptor subfamily 2, group F, member 6 (NR2F6), mGFP tagged||
|RC201336L3||Lenti ORF clone of Human nuclear receptor subfamily 2, group F, member 6 (NR2F6), Myc-DDK-tagged||
|RC201336L4||Lenti ORF clone of Human nuclear receptor subfamily 2, group F, member 6 (NR2F6), mGFP tagged||