Transmembrane Protein 101 (TMEM101) (NM_032376) Human Tagged ORF Clone

CAT#: RC201329

TMEM101 (Myc-DDK-tagged)-Human transmembrane protein 101 (TMEM101)

Product Images

Circular map for RC201329

• 

  Western blot validation of overexpression lysate (Cat# LY410169) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201329 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Transmembrane Protein 101
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201329 representing NM_032376
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCGAAGATAGGTTCGAGACGGTGGATGTTGCAGCTGATCATGCAGTTGGGTTCGGTGCTGCTCA
  CACGCTGCCCCTTTTGGGGCTGCTTCAGCCAGCTCATGCTGTACGCTGAGAGGGCTGAGGCACGCCGGAA
  GCCCGACATCCCAGTGCCTTACCTGTATTTCGACATGGGGGCAGCCGTGCTGTGCGCTAGTTTCATGTCC
  TTTGGCGTGAAGCGGCGCTGGTTCGCGCTGGGGGCCGCACTCCAATTGGCCATTAGCACCTACGCCGCCT
  ACATCGGGGGCTACGTCCACTACGGGGACTGGCTGAAGGTCCGTATGTACTCGCGCACAGTTGCCATCAT
  CGGCGGCTTTCTTGTGTTGGCCAGCGGTGCTGGGGAGCTGTACCGCCGGAAACCTCGCAGCCGCTCCCTG
  CAGTCCACCGGCCAGGTGTTCCTGGGTATCTACCTCATCTGTGTGGCCTACTCACTGCAGCACAGCAAGG
  AGGACCGGCTGGCGTATCTGAACCATCTCCCAGGAGGGGAGCTGATGATCCAGCTGTTCTTCGTGCTGTA
  TGGCATCCTGGCCCTGGCCTTTCTGTCAGGCTACTACGTGACCCTCGCTGCCCAGATCCTGGCTGTACTG
  CTGCCCCCTGTCATGCTGCTCATTGATGGCAATGTTGCTTACTGGCACAACACGCGGCGTGTTGAGTTCT
  GGAACCAGATGAAGCTCCTTGGAGAGAGTGTGGGCATCTTCGGAACTGCTGTCATCCTGGCCACTGATGG
  C
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201329 representing NM_032376
  Red=Cloning site Green=Tags(s)
  
  MASKIGSRRWMLQLIMQLGSVLLTRCPFWGCFSQLMLYAERAEARRKPDIPVPYLYFDMGAAVLCASFMS
  FGVKRRWFALGAALQLAISTYAAYIGGYVHYGDWLKVRMYSRTVAIIGGFLVLASGAGELYRRKPRSRSL
  QSTGQVFLGIYLICVAYSLQHSKEDRLAYLNHLPGGELMIQLFFVLYGILALAFLSGYYVTLAAQILAVL
  LPPVMLLIDGNVAYWHNTRRVEFWNQMKLLGESVGIFGTAVILATDG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032376
• ORF Size: 771 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032376.4
• RefSeq Size: 1563 bp
• RefSeq ORF: 774 bp
• Locus ID: 84336
• UniProt ID: Q96IK0
• Cytogenetics: 17q21.31
• Protein Families: Transmembrane
• MW: 28.6 kDa
• Gene Summary: May activate NF-kappa-B signaling pathways.[UniProtKB/Swiss-Prot Function]