C20orf4 (AAR2) (NM_015511) Human Tagged ORF Clone

CAT#: RC201316

AAR2 (Myc-DDK-tagged)-Human chromosome 20 open reading frame 4 (C20orf4)

Product Images

Circular map for RC201316

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  Western blot validation of overexpression lysate (Cat# LY414504) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201316 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified AAR2 protein (Cat# TP301316). The protein was produced from HEK293T cells transfected with AAR2 cDNA clone (Cat# RC201316) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: C20orf4
• Synonyms: C20orf4; CGI-23
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201316 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCCGTGCAGATGGATCCTGAGCTAGCCAAGCGCCTCTTCTTTGAAGGGGCCACTGTGGTCATCC
  TGAACATGCCCAAGGGAACAGAGTTTGGGATTGACTATAACTCCTGGGAGGTCGGGCCCAAGTTCCGGGG
  CGTGAAGATGATCCCTCCAGGCATCCACTTCCTCCACTACAGCTCTGTGGACAAGGCTAATCCGAAGGAA
  GTAGGCCCTCGTATGGGTTTCTTCCTTAGCCTGCACCAGCGGGGGCTGACAGTGCTGCGCTGGAGCACAC
  TCAGGGAAGAGGTAGACCTGTCCCCAGCCCCAGAGTCTGAGGTGGAGGCCATGAGGGCCAACCTCCAGGA
  GCTGGACCAGTTCCTGGGGCCTTACCCATATGCCACCCTGAAGAAGTGGATCTCACTCACCAACTTCATC
  AGCGAAGCCACAGTGGAGAAGCTACAGCCCGAGAATCGACAGATCTGTGCCTTTTCCGATGTGCTACCTG
  TGCTCTCCATGAAGCACACCAAGGACCGCGTGGGGCAGAATCTACCCCGCTGTGGCATTGAGTGCAAAAG
  CTACCAAGAGGGCCTGGCCCGGCTACCAGAGATGAAGCCCAGAGCCGGGACAGAGATCCGCTTCTCAGAG
  CTGCCCACGCAGATGTTCCCAGAGGGTGCCACGCCAGCTGAGATAACCAAGCACAGCATGGACCTGAGCT
  ATGCCCTGGAGACTGTGCTCAACAAGCAGTTCCCCAGCAGCCCCCAGGATGTGCTTGGTGAACTCCAGTT
  TGCTTTTGTGTGCTTCCTGCTGGGGAATGTGTACGAGGCATTTGAGCATTGGAAGCGGCTCCTGAACCTC
  CTGTGCCGGTCAGAAGCAGCCATGATGAAGCACCACACCCTCTACATCAACCTCATCTCCATCCTGTACC
  ACCAGCTTGGTGAGATCCCCGCTGACTTCTTCGTAGACATTGTCTCCCAAGACAACTTCCTCACCAGCAC
  CTTACAGGTTTTCTTTTCCTCTGCCTGCAGCATTGCCGTGGATGCCACCCTGAGAAAGAAAGCTGAAAAG
  TTCCAAGCTCACCTGACCAAGAAGTTCCGGTGGGACTTTGCTGCGGAACCTGAGGACTGTGCCCCGGTGG
  TGGTGGAGCTCCCTGAGGGCATCGAGATGGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201316 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAVQMDPELAKRLFFEGATVVILNMPKGTEFGIDYNSWEVGPKFRGVKMIPPGIHFLHYSSVDKANPKE
  VGPRMGFFLSLHQRGLTVLRWSTLREEVDLSPAPESEVEAMRANLQELDQFLGPYPYATLKKWISLTNFI
  SEATVEKLQPENRQICAFSDVLPVLSMKHTKDRVGQNLPRCGIECKSYQEGLARLPEMKPRAGTEIRFSE
  LPTQMFPEGATPAEITKHSMDLSYALETVLNKQFPSSPQDVLGELQFAFVCFLLGNVYEAFEHWKRLLNL
  LCRSEAAMMKHHTLYINLISILYHQLGEIPADFFVDIVSQDNFLTSTLQVFFSSACSIAVDATLRKKAEK
  FQAHLTKKFRWDFAAEPEDCAPVVVELPEGIEMG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015511
• ORF Size: 1152 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015511.4
• RefSeq Size: 2765 bp
• RefSeq ORF: 1155 bp
• Locus ID: 25980
• UniProt ID: Q9Y312
• Cytogenetics: 20q11.23
• MW: 43.5 kDa
• Gene Summary: This gene encodes the homolog of the yeast A1-alpha2 repressin protein that is involved in mRNA splicing. Alternately spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2012]