Bcl x (BCL2L1) (NM_138578) Human Tagged ORF Clone
BCL2L1 (Myc-DDK-tagged)-Human BCL2-like 1 (BCL2L1), nuclear gene encoding mitochondrial protein, transcript variant 1
|Product Name||Bcl x (BCL2L1) (NM_138578) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|Synonyms||Bcl-X; BCL-XL/S; BCL2L; BCLX; PPP1R52|
|E. coli Selection||Kanamycin (25 ug/mL)|
>RC201314 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC201314 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||699 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq||NM_138578.1, NM_138578.2, NP_612815.1|
|RefSeq Size||2575 bp|
|RefSeq ORF||702 bp|
|Protein Families||Druggable Genome, ES Cell Differentiation/IPS, Transmembrane|
|Protein Pathways||Amyotrophic lateral sclerosis (ALS), Apoptosis, Chronic myeloid leukemia, Jak-STAT signaling pathway, Pancreatic cancer, Pathways in cancer, Small cell lung cancer|
|Gene Summary||The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Alternative splicing results in multiple transcript variants encoding two different isoforms. The longer isoform acts as an apoptotic inhibitor and the shorter isoform acts as an apoptotic activator. [provided by RefSeq, Dec 2015]|
|The use of this cDNA Clones has been cited in the following citations:|
Novel tumor suppressor role of miRNA-876 in cholangiocarcinoma
,Ursu, S;Majid, S;Garger, C;de Semir, D;Bezrookove, V;Desprez, PY;McAllister, S;Soroceanu, L;Nosrati, M;Yimam, K;Hassoun, A;Osorio, R;Kashani-Sabet, M;Dar, AA;,
,PubMed ID 31409772
Distinct modes of cell competition shape mammalian tissue morphogenesis
,Ellis, SJ;Gomez, NC;Levorse, J;Mertz, AF;Ge, Y;Fuchs, E;,
,PubMed ID 31092920
Extra-mitochondrial prosurvival BCL-2 proteins regulate gene transcription by inhibiting the SUFU tumour suppressor
,Wu, X;Zhang, LS;Toombs, J;Kuo, YC;Piazza, JT;Tuladhar, R;Barrett, Q;Fan, CW;Zhang, X;Walensky, LD;Kool, M;Cheng, SY;Brekken, R;Opferman, JT;Green, DR;Moldoveanu, T;Lum, L;,
Nat. Cell Biol. 2017
,PubMed ID 28945232
Lemur tyrosine kinase 2 (LMTK2) is a determinant of cell sensitivity to apoptosis by regulating the levels of the BCL2 family members
,Conti, A;Majorini, MT;Fontanella, E;Bardelli, A;Giacca, M;Delia, D;Mano, M;Lecis, D;,
Cancer Lett 2016
,PubMed ID 28040547
|cDNA Clone Resources|
|SC127825||BCL2L1 (untagged)-Human BCL2-like 1 (BCL2L1), nuclear gene encoding mitochondrial protein, transcript variant 1||
|RG201314||BCL2L1 (GFP-tagged) - Human BCL2-like 1 (BCL2L1), nuclear gene encoding mitochondrial protein, transcript variant 1||
|RC201314L1||Lenti ORF clone of Human BCL2-like 1 (BCL2L1), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged||
|RC201314L2||Lenti ORF clone of Human BCL2-like 1 (BCL2L1), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged||
|RC201314L3||Lenti ORF clone of Human BCL2-like 1 (BCL2L1), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged||
|RC201314L4||Lenti ORF clone of Human BCL2-like 1 (BCL2L1), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged||