ABCD4 (NM_005050) Human Tagged ORF Clone

CAT#: RC201291

ABCD4 (Myc-DDK-tagged)-Human ATP-binding cassette, sub-family D (ALD), member 4 (ABCD4), transcript variant 1

Product Images

Circular map for RC201291

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  Western blot validation of overexpression lysate (Cat# LY417546) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201291 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ABCD4 protein (Cat# TP301291). The protein was produced from HEK293T cells transfected with ABCD4 cDNA clone (Cat# RC201291) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ABCD4
• Synonyms: ABC41; EST352188; MAHCJ; P70R; P79R; PMP69; PXMP1L
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201291 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGTCGCGGGGCCCGCGCCCGGAGCTGGCGCCAGGCCCAGGTTAGATCTGCAATTTCTCCAGCGGT
  TCCTGCAGATACTGAAGGTTTTGTTTCCTTCTTGGTCATCACAAAATGCCTTGATGTTCCTGACCCTTTT
  GTGCCTGACCCTACTGGAGCAATTTGTGATCTACCAGGTTGGCCTGATCCCCAGTCAGTACTATGGGGTC
  CTGGGAAACAAAGACTTGGAAGGGTTTAAGACTCTGACATTCCTGGCTGTCATGCTCATTGTTCTGAACT
  CCACGCTGAAGAGCTTTGATCAGTTCACCTGCAACCTGCTGTATGTGAGCTGGAGGAAGGACCTCACTGA
  GCACCTTCACCGCCTCTACTTCCGGGGCCGTGCGTACTACACCCTCAACGTGCTGCGGGATGACATCGAT
  AACCCGGACCAGCGCATCAGCCAGGACGTGGAGCGATTCTGCCGGCAGCTCAGCAGCATGGCCAGCAAGC
  TCATCATCTCCCCGTTCACCCTCGTCTACTACACTTACCAGTGCTTCCAAAGCACAGGCTGGCTCGGGCC
  TGTGAGCATCTTCGGGTATTTCATCCTGGGGACCGTGGTGAACAAAACTTTGATGGGCCCCATTGTGATG
  AAGCTGGTGCATCAGGAGAAGCTGGAGGGAGATTTTAGGTTCAAGCACATGCAGATTCGGGTGAATGCGG
  AGCCTGCTGCTTTCTACAGAGCTGGGCATGTGGAGCACATGAGGACAGACCGCAGGCTGCAGAGACTCCT
  TCAGACCCAGAGGGAGCTGATGTCCAAGGAGCTCTGGCTGTACATCGGCATCAACACCTTTGACTATCTG
  GGCAGCATCCTGAGTTACGTTGTCATCGCAATCCCCATTTTCAGCGGGGTCTATGGAGACCTGAGTCCCA
  CAGAGCTTAGCACCCTGGTCAGCAAGAATGCCTTTGTGTGCATCTACCTCATCAGCTGCTTCACCCAGCT
  AATCGACCTGTCCACGACGCTCTCAGATGTGGCTGGCTACACGCACAGAATTGGGCAGCTTCGGGAGACG
  CTTCTGGACATGTCCCTGAAGTCACAGGACTGCGAGATCCTGGGCGAGAGCAAGTGGGGCTTGGACACAC
  CCCCAGGGTGGCCAGCGGCAGAGCCAGCAGACACAGCATTTCTCCTTGAGCGGGTCTCCATCTCTGCCCC
  CTCCTCTGACAAACCCCTAATCAAGGATCTGAGCCTAAAGATCTCCGAGGGACAGAGCCTGCTCATCACA
  GGCAACACGGGCACTGGCAAGACCTCCTTGCTCCGGGTTCTGGGTGGCCTCTGGACGAGTACACGGGGCT
  CAGTGCAGATGCTGACGGACTTTGGGCCCCATGGGGTGCTATTCCTGCCACAAAAGCCATTCTTCACTGA
  CGGGACCCTTCGGGAGCAGGTGATATATCCCCTGAAGGAGGTCTACCCCGACTCAGGTTCTGCCGATGAT
  GAGAGGATCTTGAGGTTCTTGGAATTGGCAGGCCTGTCCAACTTGGTGGCAAGGACAGAGGGCCTGGACC
  AGCAGGTGGACTGGAACTGGTATGATGTTCTGTCCCCGGGGGAGATGCAACGGCTCTCCTTTGCCCGACT
  CTTCTACCTGCAGCCGAAGTACGCAGTGCTTGATGAAGCCACCAGTGCCCTGACAGAGGAAGTGGAGAGC
  GAGCTCTATCGCATCGGCCAGCAGCTGGGGATGACGTTCATCAGTGTGGGACATCGGCAGAGCCTTGAGA
  AGTTTCATTCCTTGGTTCTGAAACTCTGTGGAGGAGGAAGATGGGAGCTGATGAGAATCAAAGTGGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201291 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAVAGPAPGAGARPRLDLQFLQRFLQILKVLFPSWSSQNALMFLTLLCLTLLEQFVIYQVGLIPSQYYGV
  LGNKDLEGFKTLTFLAVMLIVLNSTLKSFDQFTCNLLYVSWRKDLTEHLHRLYFRGRAYYTLNVLRDDID
  NPDQRISQDVERFCRQLSSMASKLIISPFTLVYYTYQCFQSTGWLGPVSIFGYFILGTVVNKTLMGPIVM
  KLVHQEKLEGDFRFKHMQIRVNAEPAAFYRAGHVEHMRTDRRLQRLLQTQRELMSKELWLYIGINTFDYL
  GSILSYVVIAIPIFSGVYGDLSPTELSTLVSKNAFVCIYLISCFTQLIDLSTTLSDVAGYTHRIGQLRET
  LLDMSLKSQDCEILGESKWGLDTPPGWPAAEPADTAFLLERVSISAPSSDKPLIKDLSLKISEGQSLLIT
  GNTGTGKTSLLRVLGGLWTSTRGSVQMLTDFGPHGVLFLPQKPFFTDGTLREQVIYPLKEVYPDSGSADD
  ERILRFLELAGLSNLVARTEGLDQQVDWNWYDVLSPGEMQRLSFARLFYLQPKYAVLDEATSALTEEVES
  ELYRIGQQLGMTFISVGHRQSLEKFHSLVLKLCGGGRWELMRIKVE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005050
• ORF Size: 1818 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005050.4
• RefSeq Size: 3157 bp
• RefSeq ORF: 1821 bp
• Locus ID: 5826
• UniProt ID: O14678
• Cytogenetics: 14q24.3
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: ABC transporters
• MW: 68.6 kDa
• Gene Summary: The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ALD subfamily, which is involved in peroxisomal import of fatty acids and/or fatty acyl-CoAs in the organelle. All known peroxisomal ABC transporters are half transporters which require a partner half transporter molecule to form a functional homodimeric or heterodimeric transporter. The function of this peroxisomal membrane protein is unknown. However, it is speculated that it may function as a heterodimer for another peroxisomal ABC transporter and, therefore, may modify the adrenoleukodystrophy phenotype. It may also play a role in the process of peroxisome biogenesis. Alternative splicing results in several protein-coding and non-protein-coding variants. [provided by RefSeq, Jul 2017]