HIGD2A (NM_138820) Human Tagged ORF Clone
HIGD2A (Myc-DDK-tagged)-Human HIG1 hypoxia inducible domain family, member 2A (HIGD2A)
|Type||Human Tagged ORF Clone|
|E. coli Selection||Kanamycin (25 ug/mL)|
|Mammalian Cell Selection||Neomycin|
>RC201223 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC201223 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||318 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||628 bp|
|RefSeq ORF||321 bp|
|Gene Summary||The protein encoded by this gene is a subunit of the cytochrome c oxidase complex (complex IV), which is the terminal enzyme in the mitochondrial respiratory chain. The encoded protein is an inner mitochondrial membrane protein and is a functional ortholog of the yeast respiratory supercomplex factor 1 (Rcf1). In mouse, the orthologous protein enhances cell survival under conditions of hypoxia. [provided by RefSeq, Sep 2016]|
|cDNA Clone Resources|
|RC201223L3||Lenti ORF clone of Human HIG1 hypoxia inducible domain family, member 2A (HIGD2A), Myc-DDK-tagged||
|RC201223L4||Lenti ORF clone of Human HIG1 hypoxia inducible domain family, member 2A (HIGD2A), mGFP tagged||
|RG201223||HIGD2A (tGFP-tagged) - Human HIG1 hypoxia inducible domain family, member 2A (HIGD2A)||
|SC120662||HIGD2A (untagged)-Human HIG1 hypoxia inducible domain family, member 2A (HIGD2A)||