PSMD11 (NM_002815) Human Tagged ORF Clone

CAT#: RC201201

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PSMD11 (Myc-DDK-tagged)-Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 11 (PSMD11)



  "NM_002815" in other vectors (6)

Reconstitution Protocol

USD 457.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol PSMD11
Synonyms p44.5; Rpn6; S9
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC201201 representing NM_002815
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCGGCGGCGGTGGTGGAGTTCCAGAGAGCCCAGTCTCTACTCAGCACCGACCGGGAGGCCTCCA
TCGACATCCTCCACTCCATCGTGAAGCGTGACATTCAGGAAAACGATGAAGAGGCAGTGCAAGTCAAAGA
GCAGAGCATCCTGGAACTGGGATCTCTCCTGGCAAAGACTGGACAAGCTGCAGAGCTTGGAGGACTCCTG
AAGTATGTACGACCCTTCTTGAATTCCATCAGCAAGGCTAAAGCAGCTCGCCTGGTCCGATCTCTTCTTG
ATCTGTTTCTTGATATGGAAGCAGCTACAGGGCAGGAGGTCGAGCTGTGTTTAGAGTGCATCGAATGGGC
CAAGTCAGAGAAAAGAACTTTCTTACGCCAAGCTTTGGAGGCAAGACTGGTGTCTTTGTACTTTGATACC
AAGAGGTACCAGGAAGCATTGCATTTGGGTTCTCAGCTGCTGCGGGAGTTGAAAAAGATGGACGACAAAG
CTCTTTTGGTGGAAGTACAGCTTTTAGAAAGCAAAACATACCATGCCCTGAGCAACCTGCCGAAAGCCCG
AGCTGCCTTAACTTCTGCTCGAACCACAGCAAATGCCATCTACTGCCCCCCTAAATTGCAGGCCACCTTG
GACATGCAGTCGGGTATTATCCATGCAGCAGAAGAGAAGGACTGGAAAACTGCGTACTCATACTTCTATG
AGGCATTTGAGGGTTATGACTCCATCGACAGCCCCAAGGCCATCACATCTCTGAAGTACATGTTGCTGTG
CAAAATCATGCTCAACACCCCAGAAGATGTCCAGGCTTTGGTGAGCGGGAAGCTTGCACTTCGGTATGCA
GGGAGGCAGACAGAAGCATTAAAATGCGTGGCTCAGGCTAGCAAGAACAGATCACTGGCAGATTTTGAAA
AGGCTCTGACAGATTACCGGGCAGAGCTCCGGGATGACCCAATCATCAGCACACACTTGGCCAAGTTGTA
TGATAACTTACTAGAACAGAATCTGATCCGAGTCATTGAGCCTTTTTCCAGAGTACAGATTGAACACATA
TCTAGTCTCATCAAACTCTCCAAGGCCGACGTGGAAAGGAAATTATCACAGATGATTCTTGACAAGAAAT
TTCATGGGATTTTGGACCAGGGGGAGGGTGTCCTGATTATTTTCGATGAACCCCCAGTAGATAAAACTTA
CGAAGCTGCTCTGGAAACAATTCAGAACATGAGCAAAGTAGTGGATTCCCTCTACAACAAAGCCAAGAAA
CTGACA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC201201 representing NM_002815
Red=Cloning site Green=Tags(s)

MAAAAVVEFQRAQSLLSTDREASIDILHSIVKRDIQENDEEAVQVKEQSILELGSLLAKTGQAAELGGLL
KYVRPFLNSISKAKAARLVRSLLDLFLDMEAATGQEVELCLECIEWAKSEKRTFLRQALEARLVSLYFDT
KRYQEALHLGSQLLRELKKMDDKALLVEVQLLESKTYHALSNLPKARAALTSARTTANAIYCPPKLQATL
DMQSGIIHAAEEKDWKTAYSYFYEAFEGYDSIDSPKAITSLKYMLLCKIMLNTPEDVQALVSGKLALRYA
GRQTEALKCVAQASKNRSLADFEKALTDYRAELRDDPIISTHLAKLYDNLLEQNLIRVIEPFSRVQIEHI
SSLIKLSKADVERKLSQMILDKKFHGILDQGEGVLIIFDEPPVDKTYEAALETIQNMSKVVDSLYNKAKK
LT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_002815
ORF Size 1266 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002815.4
RefSeq Size 1598 bp
RefSeq ORF 1269 bp
Locus ID 5717
UniProt ID O00231
Cytogenetics 17q11.2
Domains PCI
Protein Families Stem cell - Pluripotency
Protein Pathways Proteasome
MW 47.3 kDa
Gene Summary The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This gene encodes a member of the proteasome subunit S9 family that functions as a non-ATPase subunit of the 19S regulator and is phosphorylated by AMP-activated protein kinase. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jul 2012]

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