Phosphoribosyl pyrophosphate amidotransferase (PPAT) (NM_002703) Human Tagged ORF Clone

CAT#: RC201144

PPAT (Myc-DDK-tagged)-Human phosphoribosyl pyrophosphate amidotransferase (PPAT)

Product Images

Circular map for RC201144

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PPAT (Cat# RC201144, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPAT(Cat# TA504769). Positive lysates LY400951 (100ug) and LC400951 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY400951) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201144 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified PPAT protein (Cat# TP301144). The protein was produced from HEK293T cells transfected with PPAT cDNA clone (Cat# RC201144) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Phosphoribosyl pyrophosphate amidotransferase
• Synonyms: ATASE; GPAT; PRAT
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201144 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTGGAGGAGTTGGGGATCCGAGAGGAATGTGGCGTGTTCGGGTGCATCGCCTCAGGAGAGTGGC
  CCACGCAGCTGGATGTACCGCATGTGATCACTCTGGGACTCGTGGGGCTGCAGCACCGGGGTCAGGAGAG
  TGCTGGTATTGTGACTAGTGATGGGAGTTCCGTGCCAACATTCAAATCACACAAGGGAATGGGTCTTGTA
  AATCACGTCTTTACTGAAGACAATTTGAAAAAATTATATGTTTCAAATCTTGGAATTGGACACACCAGGT
  ATGCCACCACAGGAAAATGTGAACTAGAAAATTGTCAGCCCTTCGTTGTTGAAACACTTCATGGGAAGAT
  AGCTGTGGCACATAATGGCGAATTGGTAAATGCTGCTCGATTAAGGAAAAAGCTTCTGCGTCATGGTATT
  GGTCTGTCTACAAGTTCTGATAGTGAAATGATTACCCAGTTACTGGCGTATACCCCTCCTCAGGAACAAG
  ATGACACCCCAGACTGGGTAGCCAGGATTAAAAACTTGATGAAGGAAGCACCCACAGCATACTCCCTGCT
  TATAATGCACAGAGATGTTATTTATGCAGTACGAGATCCTTATGGAAATCGTCCCTTATGCATTGGTCGT
  CTTATTCCAGTGTCTGATATAAATGACAAAGAGAAAAAAACATCAGAAACAGAAGGATGGGTGGTGTCTT
  CAGAATCTTGTAGCTTCTTATCTATTGGTGCAAGATATTACCGTGAAGTCTTGCCTGGAGAAATTGTGGA
  AATATCCAGACACAATGTCCAAACTCTTGATATTATATCAAGGTCTGAAGGAAACCCAGTGGCTTTTTGT
  ATCTTTGAATATGTTTATTTTGCAAGACCAGACAGTATGTTCGAAGACCAAATGGTTTATACAGTAAGAT
  ACCGTTGTGGCCAGCAGCTAGCGATTGAAGCACCTGTGGATGCAGATTTGGTTAGCACTGTTCCAGAATC
  TGCTACGCCTGCTGCTCTTGCTTACGCAGGAAAGTGTGGACTTCCATATGTGGAGGTGCTGTGTAAAAAC
  CGGTATGTAGGGAGAACCTTCATTCAGCCAAACATGAGGTTAAGACAACTTGGTGTTGCAAAAAAATTTG
  GAGTATTGTCAGACAACTTTAAAGGCAAAAGAATTGTTCTTGTAGATGATTCAATTGTCAGAGGCAATAC
  CATCTCACCTATAATAAAACTGCTCAAAGAATCTGGTGCAAAAGAGGTACACATTCGAGTAGCTTCACCA
  CCAATTAAATATCCATGCTTCATGGGAATAAACATTCCTACAAAAGAAGAGCTCATTGCCAATAAACCAG
  AATTTGATCACCTTGCAGAATATCTAGGAGCAAACAGTGTTGTGTATCTGTCAGTAGAAGGACTGGTTTC
  ATCTGTACAAGAAGGGATAAAGTTTAAAAAACAGAAAGAGAAAAAGCACGATATTATGATCCAAGAAAAT
  GGAAATGGTCTGGAATGTTTTGAAAAGAGTGGTCATTGTACAGCTTGTCTCACTGGAAAATATCCTGTAG
  AATTAGAATGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201144 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MELEELGIREECGVFGCIASGEWPTQLDVPHVITLGLVGLQHRGQESAGIVTSDGSSVPTFKSHKGMGLV
  NHVFTEDNLKKLYVSNLGIGHTRYATTGKCELENCQPFVVETLHGKIAVAHNGELVNAARLRKKLLRHGI
  GLSTSSDSEMITQLLAYTPPQEQDDTPDWVARIKNLMKEAPTAYSLLIMHRDVIYAVRDPYGNRPLCIGR
  LIPVSDINDKEKKTSETEGWVVSSESCSFLSIGARYYREVLPGEIVEISRHNVQTLDIISRSEGNPVAFC
  IFEYVYFARPDSMFEDQMVYTVRYRCGQQLAIEAPVDADLVSTVPESATPAALAYAGKCGLPYVEVLCKN
  RYVGRTFIQPNMRLRQLGVAKKFGVLSDNFKGKRIVLVDDSIVRGNTISPIIKLLKESGAKEVHIRVASP
  PIKYPCFMGINIPTKEELIANKPEFDHLAEYLGANSVVYLSVEGLVSSVQEGIKFKKQKEKKHDIMIQEN
  GNGLECFEKSGHCTACLTGKYPVELEW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002703
• ORF Size: 1551 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002703.5
• RefSeq Size: 3713 bp
• RefSeq ORF: 1554 bp
• Locus ID: 5471
• UniProt ID: Q06203
• Cytogenetics: 4q12
• Domains: GATase_2, Pribosyltran
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Metabolic pathways, Purine metabolism
• MW: 57.4 kDa
• Gene Summary: The protein encoded by this gene is a member of the purine/pyrimidine phosphoribosyltransferase family. It is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosythetic pathway. This gene and PAICS/AIRC gene, a bifunctional enzyme catalyzing steps six and seven of this pathway, are located in close proximity on chromosome 4, and divergently transcribed from an intergenic region. [provided by RefSeq, Mar 2011]