Cytokeratin 7 (KRT7) (NM_005556) Human Tagged ORF Clone

CAT#: RC201124

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KRT7 (Myc-DDK-tagged)-Human keratin 7 (KRT7)



  "NM_005556" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 477.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol KRT7
Synonyms CK7; K2C7; K7; SCL
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC201124 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCATCCACTTCAGCTCCCCGGTATTCACCTCGCGCTCAGCCGCCTTCTCGGGCCGCGGCGCCCAGG
TGCGCCTGAGCTCCGCTCGCCCCGGCGGCCTTGGCAGCAGCAGCCTCTACGGCCTCGGCGCCTCGCGGCC
GCGCGTGGCCGTGCGCTCTGCCTATGGGGGCCCGGTGGGCGCCGGCATCCGCGAGGTCACCATTAACCAG
AGCCTGCTGGCCCCGCTGCGGCTGGACGCCGACCCCTCCCTCCAGCGGGTGCGCCAGGAGGAGAGCGAGC
AGATCAAGACCCTCAACAACAAGTTTGCCTCCTTCATCGACAAGGTGCGGTTTCTGGAGCAGCAGAACAA
GCTGCTGGAGACCAAGTGGACGCTGCTGCAGGAGCAGAAGTCGGCCAAGAGCAGCCGCCTCCCAGACATC
TTTGAGGCCCAGATTGCTGGCCTTCGGGGTCAGCTTGAGGCACTGCAGGTGGATGGGGGCCGCCTGGAGG
CGGAGCTGCGGAGCATGCAGGATGTGGTGGAGGACTTCAAGAATAAGTACGAAGATGAAATTAACCGCCG
CACAGCTGCTGAGAATGAGTTTGTGGTGCTGAAGAAGGATGTGGATGCTGCCTACATGAGCAAGGTGGAG
CTGGAGGCCAAGGTGGATGCCCTGAATGATGAGATCAACTTCCTCAGGACCCTCAATGAGACGGAGTTGA
CAGAGCTGCAGTCCCAGATCTCCGACACATCTGTGGTGCTGTCCATGGACAACAGTCGCTCCCTGGACCT
GGACGGCATCATCGCTGAGGTCAAGGCACAGTATGAGGAGATGGCCAAATGCAGCCGGGCTGAGGCTGAA
GCCTGGTACCAGACCAAGTTTGAGACCCTCCAGGCCCAGGCTGGGAAGCATGGGGACGACCTCCGGAATA
CCCGGAATGAGATTTCAGAGATGAACCGGGCCATCCAGAGGCTGCAGGCTGAGATCGACAACATCAAGAA
CCAGCGTGCCAAGTTGGAGGCCGCCATTGCCGAGGCTGAGGAGCGTGGGGAGCTGGCGCTCAAGGATGCT
CGTGCCAAGCAGGAGGAGCTGGAAGCCGCCCTGCAGCGGGCCAAGCAGGATATGGCACGGCAGCTGCGTG
AGTACCAGGAACTCATGAGCGTGAAGCTGGCCCTGGACATCGAGATCGCCACCTACCGCAAGCTGCTGGA
GGGCGAGGAGAGCCGGTTGGCTGGAGATGGAGTGGGAGCCGTGAATATCTCTGTGATGAATTCCACTGGT
GGCAGTAGCAGTGGCGGTGGCATTGGGCTGACCCTCGGGGGAACCATGGGCAGCAATGCCCTGAGCTTCT
CCAGCAGTGCGGGTCCTGGGCTCCTGAAGGCTTATTCCATCCGGACCGCATCCGCCAGTCGCAGGAGTGC
CCGCGAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC201124 protein sequence
Red=Cloning site Green=Tags(s)

MSIHFSSPVFTSRSAAFSGRGAQVRLSSARPGGLGSSSLYGLGASRPRVAVRSAYGGPVGAGIREVTINQ
SLLAPLRLDADPSLQRVRQEESEQIKTLNNKFASFIDKVRFLEQQNKLLETKWTLLQEQKSAKSSRLPDI
FEAQIAGLRGQLEALQVDGGRLEAELRSMQDVVEDFKNKYEDEINRRTAAENEFVVLKKDVDAAYMSKVE
LEAKVDALNDEINFLRTLNETELTELQSQISDTSVVLSMDNSRSLDLDGIIAEVKAQYEEMAKCSRAEAE
AWYQTKFETLQAQAGKHGDDLRNTRNEISEMNRAIQRLQAEIDNIKNQRAKLEAAIAEAEERGELALKDA
RAKQEELEAALQRAKQDMARQLREYQELMSVKLALDIEIATYRKLLEGEESRLAGDGVGAVNISVMNSTG
GSSSGGGIGLTLGGTMGSNALSFSSSAGPGLLKAYSIRTASASRRSARD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_005556
ORF Size 1407 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_005556.2
RefSeq Size 1753 bp
RefSeq ORF 1410 bp
Locus ID 3855
UniProt ID P08729
Cytogenetics 12q13.13
Domains filament
Protein Families ES Cell Differentiation/IPS
MW 51.4 kDa
Gene Summary The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. [provided by RefSeq, Jul 2008]

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