PRA1 (RABAC1) (NM_006423) Human Tagged ORF Clone

CAT#: RC201112

RABAC1 (Myc-DDK-tagged)-Human Rab acceptor 1 (prenylated) (RABAC1)

Product Images

Circular map for RC201112

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  Western blot validation of overexpression lysate (Cat# LY401932) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201112 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PRA1
• Synonyms: PRA1; PRAF1; YIP3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201112 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCGCAGAAGGACCAGCAGAAAGATGCCGAGGCGGAAGGGCTGAGCGGCACGACCCTGCTGCCGA
  AGCTGATTCCCTCCGGTGCAGGCCGGGAGTGGCTGGAGCGGCGCCGCGCGACCATCCGGCCCTGGAGCAC
  CTTCGTGGACCAGCAGCGCTTCTCACGGCCCCGCAACCTGGGAGAGCTGTGCCAGCGCCTCGTACGCAAC
  GTGGAGTACTACCAGAGCAACTATGTGTTCGTGTTCCTGGGCCTCATCCTGTACTGTGTGGTGACGTCCC
  CTATGTTGCTGGTGGCTCTGGCTGTCTTTTTCGGCGCCTGTTACATTCTCTATCTGCGCACCTTGGAGTC
  CAAGCTTGTGCTCTTTGGCCGAGAGGTGAGCCCAGCGCATCAGTATGCTCTGGCTGGAGGCATCTCCTTC
  CCCTTCTTCTGGCTGGCTGGTGCGGGCTCGGCCGTCTTCTGGGTGCTGGGAGCCACCCTGGTGGTCATCG
  GCTCCCACGCTGCCTTCCACCAGATTGAGGCTGTGGACGGGGAGGAGCTGCAGATGGAACCCGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201112 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAQKDQQKDAEAEGLSGTTLLPKLIPSGAGREWLERRRATIRPWSTFVDQQRFSRPRNLGELCQRLVRN
  VEYYQSNYVFVFLGLILYCVVTSPMLLVALAVFFGACYILYLRTLESKLVLFGREVSPAHQYALAGGISF
  PFFWLAGAGSAVFWVLGATLVVIGSHAAFHQIEAVDGEELQMEPV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006423
• ORF Size: 555 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006423.3
• RefSeq Size: 821 bp
• RefSeq ORF: 558 bp
• Locus ID: 10567
• UniProt ID: Q9UI14
• Cytogenetics: 19q13.2
• Domains: PRA1
• Protein Families: Transmembrane
• MW: 20.6 kDa
• Gene Summary: General Rab protein regulator required for vesicle formation from the Golgi complex. May control vesicle docking and fusion by mediating the action of Rab GTPases to the SNARE complexes. In addition it inhibits the removal of Rab GTPases from the membrane by GDI.[UniProtKB/Swiss-Prot Function]