GLI1 (NM_005269) Human Tagged ORF Clone
Lenti ORF clone of Human GLI family zinc finger 1 (GLI1), transcript variant 1, Myc-DDK-tagged
|Product Name||GLI1 (NM_005269) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|Synonyms||GLI; PAPA8; PPD1|
|E. coli Selection||Chloramphenicol (34 ug/mL)|
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene|
|ORF Size||3318 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||3618 bp|
|RefSeq ORF||3321 bp|
|Protein Families||Adult stem cells, Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Stem cell relevant signaling - DSL/Notch pathway, Transcription Factors|
|Protein Pathways||Basal cell carcinoma, Hedgehog signaling pathway, Pathways in cancer|
|Gene Summary||This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]|
|cDNA Clone Resources|
|SC125780||GLI1 (untagged)-Human GLI family zinc finger 1 (GLI1), transcript variant 1||
|RC201110||GLI1 (Myc-DDK-tagged)-Human GLI family zinc finger 1 (GLI1), transcript variant 1||
|RG201110||GLI1 (GFP-tagged) - Human GLI family zinc finger 1 (GLI1), transcript variant 1||
|RC201110L1||Lenti ORF clone of Human GLI family zinc finger 1 (GLI1), transcript variant 1, Myc-DDK-tagged||
|RC201110L2||Lenti ORF clone of Human GLI family zinc finger 1 (GLI1), transcript variant 1, mGFP tagged||
|RC201110L4||Lenti ORF clone of Human GLI family zinc finger 1 (GLI1), transcript variant 1, mGFP tagged||