GLI1 (NM_005269) Human Tagged ORF Clone

CAT#: RC201110L2

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  • LentiORF®

Lenti ORF clone of Human GLI family zinc finger 1 (GLI1), transcript variant 1, mGFP tagged



  "NM_005269" in other vectors (6)

Reward yourself with an Amazon® Card when get any 3 cDNA clones.

USD 1,200.00


Availability*
3 Weeks

Size
    • 10 ug


Product images

Specifications

Product Data
Product Name GLI1 (NM_005269) Human Tagged ORF Clone
Type Human Tagged ORF Clone
Tag mGFP
Symbol GLI1
Synonyms GLI; PAPA8; PPD1
Vector pLenti-C-mGFP
E. coli Selection Chloramphenicol (34 ug/mL)
Cell Selection None
Sequence Data
The ORF insert of this clone is exactly the same as(RC201110).
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NM_005269
ORF Size 3318 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_005269.1, NP_005260.1
RefSeq Size 3618 bp
RefSeq ORF 3321 bp
Locus ID 2735
Cytogenetics 12q13.3
Protein Families Adult stem cells, Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Stem cell relevant signaling - DSL/Notch pathway, Transcription Factors
Protein Pathways Basal cell carcinoma, Hedgehog signaling pathway, Pathways in cancer
MW 117.9 kDa
Gene Summary This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]

Other Versions

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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