valyl tRNA synthetase (VARS) (NM_006295) Human Tagged ORF Clone

CAT#: RC201089

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VARS (Myc-DDK-tagged)-Human valyl-tRNA synthetase (VARS), nuclear gene encoding mitochondrial protein


  "NM_006295" in other vectors (6)

USD 98.00

USD 800.00


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Size
    • 10 ug


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Specifications

Product Data
Product Name valyl tRNA synthetase (VARS) (NM_006295) Human Tagged ORF Clone
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol VARS
Synonyms G7A; NDMSCA; VARS1; VARS2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Cell Selection Neomycin
Sequence Data
>RC201089 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCACCCTCTACGTCTCCCCTCACCCAGATGCCTTCCCCAGCCTCCGAGCCCTCATAGCCGCTCGCT
ATGGGGAGGCTGGGGAGGGTCCCGGATGGGGAGGAGCCCACCCCCGCATCTGTCTCCAGCCACCCCCGAC
TAGCAGGACTAGCTTTCCCCCACCCCGCCTGCCGGCCCTGGAGCAGGGGCCCGGTGGGCTCTGGGTGTGG
GGGGCCACGGCTGTGGCCCAGCTGCTGTGGCCAGCAGGCCTGGGGGGCCCAGGGGGCAGCCGGGCGGCTG
TCCTTGTCCAACAGTGGGTCAGTTACGCCGACACGGAGTTAATACCAGCTGCCTGTGGAGCAACGCTGCC
GGCCCTGGGACTCCGAAGCTCGGCCCAGGACCCCCAGGCTGTGCTGGGGGCCCTGGGCAGGGCCCTGAGC
CCCTTGGAGGAGTGGCTTCGGCTGCACACCTACTTGGCCGGGGAGGCCCCCACTCTGGCTGACCTGGCGG
CTGTCACAGCCTTGCTGCTGCCTTTCCGATACGTCCTAGACCCACCTGCCCGCCGGATCTGGAATAATGT
GACTCGCTGGTTTGTCACGTGTGTCCGGCAGCCAGAATTCCGAGCCGTGCTAGGAGAAGTGGTTCTATAC
TCAGGAGCCAGGCCTCTCTCTCATCAGCCAGGCCCCGAGGCTCCTGCCCTCCCAAAGACAGCTGCTCAGC
TCAAGAAAGAGGCAAAGAAACGGGAGAAGCTAGAGAAATTCCAACAGAAGCAGAAGATCCAACAGCAGCA
GCCACCTCCAGGGGAGAAGAAACCAAAACCAGAGAAGAGGGAGAAACGGGATCCTGGGGTCATTACCTAT
GACCTCCCAACCCCACCCGGGGAAAAGAAAGATGTCAGTGGCCCCATGCCCGACTCCTACAGCCCTCGGT
ATGTGGAGGCTGCCTGGTACCCTTGGTGGGAGCAGCAGGGCTTCTTCAAGCCAGAGTATGGGCGTCCTAA
TGTGTCAGCAGCAAATCCCCGAGGTGTCTTCATGATGTGCATCCCACCCCCCAATGTGACAGGCTCCCTG
CACCTGGGCCATGCACTCACCAACGCCATCCAGGACTCCCTGACTCGATGGCACCGCATGCGTGGGGAGA
CCACCCTGTGGAACCCTGGCTGTGACCATGCAGGTATTGCCACCCAGGTGGTGGTGGAGAAGAAGCTATG
GCGTGAGCAGGGACTGAGCCGGCACCAGCTGGGCCGCGAGGCCTTTCTACAGGAAGTCTGGAAGTGGAAG
GAGGAGAAAGGTGACCGGATTTACCACCAGTTGAAGAAGCTTGGCAGCTCCTTGGACTGGGATCGAGCCT
GTTTTACCATGGACCCTAAACTCTCAGCAGCTGTGACAGAGGCCTTTGTCCGGCTTCACGAGGAAGGCAT
CATCTATCGCAGTACCCGCCTTGTTAACTGGTCCTGCACCCTCAACTCCGCCATCTCTGACATTGAGGTG
GATAAGAAGGAGCTGACAGGTCGCACCCTGCTCTCCGTGCCTGGCTACAAGGAGAAGGTGGAGTTCGGGG
TCCTCGTGTCCTTTGCCTATAAGGTCCAAGGCTCAGATAGCGACGAGGAGGTGGTGGTGGCAACAACTCG
GATCGAGACAATGCTGGGAGATGTGGCTGTAGCTGTGCACCCCAAAGATACCAGATACCAGCACCTGAAG
GGGAAGAACGTGATCCACCCATTCCTGTCTCGGAGCCTTCCCATTGTCTTCGATGAATTTGTGGACATGG
ACTTTGGCACAGGTGCTGTGAAGATCACCCCCGCACATGACCAAAATGACTATGAAGTTGGGCAGCGGCA
CGGGCTGGAGGCCATCAGCATCATGGACTCCCGGGGGGCCCTCATCAATGTGCCTCCGCCTTTCCTGGGC
CTGCCCAGGTTTGAGGCCAGGAAAGCGGTGCTGGTGGCGCTGAAGGAGCGGGGACTGTTCCGTGGCATTG
AGGACAACCCCATGGTGGTGCCACTTTGCAACCGGTCGAAGGACGTGGTAGAGCCTCTGCTGCGGCCGCA
GTGGTACGTTCGCTGCGGGGAGATGGCCCAGGCTGCCAGCGCCGCTGTGACTCGGGGTGACCTCCGCATC
CTGCCTGAGGCCCATCAGCGCACATGGCATGCCTGGATGGACAACATCCGGGAGTGGTGCATTTCCAGGC
AGCTGTGGTGGGGCCATCGCATCCCAGCCTACTTTGTCACTGTCAGTGACCCAGCGGTGCCCCCTGGGGA
GGACCCTGATGGGCGGTACTGGGTGAGTGGACGCAATGAGGCGGAGGCCCGGGAGAAGGCAGCCAAGGAG
TTCGGAGTGTCCCCTGACAAGATCAGTCTCCAGCAAGATGAGGATGTATTGGATACCTGGTTCTCCTCTG
GCCTCTTCCCCTTATCCATTTTGGGCTGGCCCAACCAGTCAGAAGACCTGAGTGTGTTCTACCCCGGGAC
ACTGCTGGAGACCGGTCATGACATCCTCTTCTTCTGGGTGGCCCGGATGGTCATGCTGGGCCTGAAGCTC
ACGGGCAGGCTGCCCTTTAGAGAGGTCTACCTCCATGCCATCGTGCGAGATGCTCACGGCCGGAAGATGA
GCAAGTCTCTAGGCAATGTCATCGATCCCCTGGACGTCATCTATGGAATCTCCCTGCAGGGCCTCCACAA
CCAGCTGCTGAACAGCAACCTGGATCCCAGCGAGGTGGAGAAGGCCAAAGAAGGGCAGAAAGCTGACTTC
CCAGCGGGGATTCCTGAATGTGGCACCGATGCTCTCCGGTTTGGATTATGTGCCTACATGTCCCAGGGTC
GTGACATCAACCTGGATGTGAACCGGATACTGGGTTACCGCCACTTCTGCAACAAGCTCTGGAATGCCAC
CAAGTTTGCCCTTCGTGGCCTTGGGAAGGGTTTTGTGCCCTCACCCACCTCCCAGCCCGGAGGCCATGAG
AGCCTGGTGGACCGCTGGATCCGCAGCCGCCTGACAGAGGCTGTGAGGCTCAGCAATCAAGGCTTCCAGG
CCTACGACTTCCCGGCCGTCACCACTGCCCAGTACAGCTTCTGGCTCTATGAGCTCTGTGATGTCTACTT
GGAGTGCCTGAAACCTGTACTGAATGGGGTGGACCAGGTGGCAGCTGAGTGTGCCCGCCAGACCCTGTAC
ACTTGCCTGGACGTTGGCCTGCGGCTGCTCTCACCCTTCATGCCCTTCGTGACGGAGGAGCTGTTCCAGA
GGCTGCCCCGGAGGATGCCGCAAGCTCCCCCTAGCCTCTGTGTTACCCCCTACCCGGAGCCCTCAGAGTG
CTCCTGGAAGGACCCCGAGGCAGAAGCCGCCCTTGAGCTGGCGCTAAGCATCACGCGAGCCGTGCGCTCC
CTGCGGGCCGACTACAACCTCACCCGGATCCGGCCTGACTGTTTCCTGGAAGTGGCGGATGAGGCCACGG
GCGCCCTGGCATCGGCGGTGTCGGGCTACGTGCAGGCCCTGGCCAGCGCAGGTGTGGTGGCTGTTCTGGC
CCTGGGGGCTCCCGCCCCCCAGGGTTGCGCTGTGGCTCTGGCTTCTGATCGCTGCTCCATCCACCTGCAG
CTTCAGGGGCTGGTGGACCCTGCACGGGAGCTGGGCAAGCTGCAAGCCAAGCGAGTTGAGGCCCAGCGGC
AGGCCCAGCGTCTGCGGGAACGCCGTGCTGCCTCGGGCTATCCTGTCAAGGTGCCGCTCGAAGTCCAGGA
GGCAGATGAAGCCAAGCTCCAACAGACAGAAGCAGAGCTCAGGAAGGTGGATGAGGCCATCGCCCTATTC
CAGAAGATGCTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC201089 protein sequence
Red=Cloning site Green=Tags(s)

MSTLYVSPHPDAFPSLRALIAARYGEAGEGPGWGGAHPRICLQPPPTSRTSFPPPRLPALEQGPGGLWVW
GATAVAQLLWPAGLGGPGGSRAAVLVQQWVSYADTELIPAACGATLPALGLRSSAQDPQAVLGALGRALS
PLEEWLRLHTYLAGEAPTLADLAAVTALLLPFRYVLDPPARRIWNNVTRWFVTCVRQPEFRAVLGEVVLY
SGARPLSHQPGPEAPALPKTAAQLKKEAKKREKLEKFQQKQKIQQQQPPPGEKKPKPEKREKRDPGVITY
DLPTPPGEKKDVSGPMPDSYSPRYVEAAWYPWWEQQGFFKPEYGRPNVSAANPRGVFMMCIPPPNVTGSL
HLGHALTNAIQDSLTRWHRMRGETTLWNPGCDHAGIATQVVVEKKLWREQGLSRHQLGREAFLQEVWKWK
EEKGDRIYHQLKKLGSSLDWDRACFTMDPKLSAAVTEAFVRLHEEGIIYRSTRLVNWSCTLNSAISDIEV
DKKELTGRTLLSVPGYKEKVEFGVLVSFAYKVQGSDSDEEVVVATTRIETMLGDVAVAVHPKDTRYQHLK
GKNVIHPFLSRSLPIVFDEFVDMDFGTGAVKITPAHDQNDYEVGQRHGLEAISIMDSRGALINVPPPFLG
LPRFEARKAVLVALKERGLFRGIEDNPMVVPLCNRSKDVVEPLLRPQWYVRCGEMAQAASAAVTRGDLRI
LPEAHQRTWHAWMDNIREWCISRQLWWGHRIPAYFVTVSDPAVPPGEDPDGRYWVSGRNEAEAREKAAKE
FGVSPDKISLQQDEDVLDTWFSSGLFPLSILGWPNQSEDLSVFYPGTLLETGHDILFFWVARMVMLGLKL
TGRLPFREVYLHAIVRDAHGRKMSKSLGNVIDPLDVIYGISLQGLHNQLLNSNLDPSEVEKAKEGQKADF
PAGIPECGTDALRFGLCAYMSQGRDINLDVNRILGYRHFCNKLWNATKFALRGLGKGFVPSPTSQPGGHE
SLVDRWIRSRLTEAVRLSNQGFQAYDFPAVTTAQYSFWLYELCDVYLECLKPVLNGVDQVAAECARQTLY
TCLDVGLRLLSPFMPFVTEELFQRLPRRMPQAPPSLCVTPYPEPSECSWKDPEAEAALELALSITRAVRS
LRADYNLTRIRPDCFLEVADEATGALASAVSGYVQALASAGVVAVLALGAPAPQGCAVALASDRCSIHLQ
LQGLVDPARELGKLQAKRVEAQRQAQRLRERRAASGYPVKVPLEVQEADEAKLQQTEAELRKVDEAIALF
QKML

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
Tag Myc-DDK
ACCN NM_006295
ORF Size 3792 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_006295.1, NM_006295.2, NP_006286.1
RefSeq Size 4308
RefSeq ORF 3795
Locus ID 7407
Cytogenetics 6p21.33
Protein Families Druggable Genome
Protein Pathways Aminoacyl-tRNA biosynthesis, Valine, leucine and isoleucine biosynthesis
MW 140.5 kDa
Gene Summary Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. The protein encoded by this gene belongs to class-I aminoacyl-tRNA synthetase family and is located in the class III region of the major histocompatibility complex. [provided by RefSeq, Jul 2008]

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