TMEM70 (NM_017866) Human Tagged ORF Clone

CAT#: RC201060

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TMEM70 (Myc-DDK-tagged)-Human transmembrane protein 70 (TMEM70), nuclear gene encoding mitochondrial protein, transcript variant 1



  "NM_017866" in other vectors (4)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 118.00

USD 429.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol TMEM70
Synonyms MC5DN2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC201060 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGTTTCTGGCGTTGGGCAGCCCGTGGGCGGTCGAACTGCCTCTCTGCGGAAGGAGGACTGCATTGT
GTGCGGCCGCCGCGCTCCGAGGTCCCCGGGCCTCTGTCTCCCGGGCGTCCTCCAGCAGCGGGCCTTCGGG
GCCGGTAGCCGGCTGGAGTACGGGGCCTTCGGGAGCCGCGCGCCTTCTCCGGCGTCCGGGTCGAGCGCAG
ATCCCTGTTTATTGGGAAGGATATGTTCGATTCTTAAATACGCCATCTGACAAATCAGAAGATGGAAGGC
TAATTTATACTGGCAATATGGCCCGAGCAGTGTTTGGTGTGAAATGTTTCTCTTATTCTACGAGTCTGAT
TGGCCTTACATTTCTGCCATACATTTTTACACAAAATAATGCTATTTCTGAAAGTGTGCCTCTGCCTATT
CAAATCATATTCTATGGCATCATGGGAAGCTTTACGGTGATCACCCCAGTGCTGCTTCACTTTATTACAA
AAGGCTATGTCATTCGATTGTACCATGAGGCCACAACAGACACTTATAAAGCCATTACCTACAATGCTAT
GCTTGCAGAAACGAGTACAGTGTTTCACCAGAATGATGTGAAGATTCCAGATGCTAAACATGTATTTACC
ACATTTTATGCTAAAACAAAATCACTGTTAGTTAATCCAGTGCTCTTTCCAAACCGTGAAGACTATATCC
ATCTAATGGGTTATGACAAAGAAGAATTTATTTTGTATATGGAAGAAACCAGTGAAGAGAAACGGCATAA
AGATGACAAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC201060 protein sequence
Red=Cloning site Green=Tags(s)

MLFLALGSPWAVELPLCGRRTALCAAAALRGPRASVSRASSSSGPSGPVAGWSTGPSGAARLLRRPGRAQ
IPVYWEGYVRFLNTPSDKSEDGRLIYTGNMARAVFGVKCFSYSTSLIGLTFLPYIFTQNNAISESVPLPI
QIIFYGIMGSFTVITPVLLHFITKGYVIRLYHEATTDTYKAITYNAMLAETSTVFHQNDVKIPDAKHVFT
TFYAKTKSLLVNPVLFPNREDYIHLMGYDKEEFILYMEETSEEKRHKDDK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_017866
ORF Size 780 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_017866.6
RefSeq Size 2096 bp
RefSeq ORF 783 bp
Locus ID 54968
UniProt ID Q9BUB7
Cytogenetics 8q21.11
Protein Families Transmembrane
MW 29 kDa
Gene Summary This gene likely encodes a mitochondrial membrane protein. The encoded protein may play a role in biogenesis of mitochondrial ATP synthase. Mutations in this gene have been associated with neonatal mitochondrial encephalocardiomyopathy due to ATP synthase deficiency. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2010]

Other Versions

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