RNF181 (NM_016494) Human Tagged ORF Clone

CAT#: RC201048

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RNF181 (Myc-DDK-tagged)-Human ring finger protein 181 (RNF181)



  "NM_016494" in other vectors (4)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 118.00

USD 429.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol RNF181
Synonyms HSPC238
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC201048 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGTCCTATTTCGATGAACACGACTGCGAGCCGTCGGACCCTGAGCAGGAGACGCGAACCAACATGC
TGCTGGAGCTCGCAAGGTCACTTTTCAATAGGATGGACTTTGAAGACTTGGGGTTGGTAGTAGATTGGGA
CCACCACCTGCCTCCACCAGCTGCCAAGACTGTGGTTGAGAACCTCCCCAGGACAGTCATCAGAGGCTCT
CAGGCTGAGCTCAAGTGCCCCGTGTGTCTTTTGGAATTTGAGGAGGAGGAGACTGCCATTGAGATGCCTT
GCCATCACCTTTTCCATTCCAGCTGCATTCTGCCCTGGCTAAGCAAGACAAATTCCTGTCCCTTGTGCCG
CTATGAGCTGCCCACTGATGACGACACTTATGAGGAGCACAGACGAGATAAGGCTCGAAAACAGCAGCAG
CAACACCGACTGGAGAACCTCCATGGAGCCATGTACACG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC201048 protein sequence
Red=Cloning site Green=Tags(s)

MASYFDEHDCEPSDPEQETRTNMLLELARSLFNRMDFEDLGLVVDWDHHLPPPAAKTVVENLPRTVIRGS
QAELKCPVCLLEFEEEETAIEMPCHHLFHSSCILPWLSKTNSCPLCRYELPTDDDTYEEHRRDKARKQQQ
QHRLENLHGAMYT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_016494
ORF Size 459 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_016494.4
RefSeq Size 716 bp
RefSeq ORF 462 bp
Locus ID 51255
UniProt ID Q9P0P0
Cytogenetics 2p11.2
Protein Families Druggable Genome
MW 17.9 kDa
Gene Summary RNF181 binds the integrin alpha-IIb (ITGA2B; MIM 607759)/beta-3 (ITGB3; MIM 173470) complex and has E3 ubiquitin ligase activity (Brophy et al., 2008 [PubMed 18331836]).[supplied by OMIM, Dec 2008]

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