ADAT1 (NM_012091) Human Tagged ORF Clone

CAT#: RC201044

ADAT1 (Myc-DDK-tagged)-Human adenosine deaminase, tRNA-specific 1 (ADAT1), transcript variant 1

Product Images

Circular map for RC201044

• 

  Western blot validation of overexpression lysate (Cat# LY415994) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201044 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ADAT1
• Synonyms: HADAT1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201044 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGACCGCGGATGAGATTGCTCAGCTATGCTATGAACACTATGGGATCAGGCTGCCCAAGAAGGGGA
  AGCCTGAGCCAAACCATGAGTGGACATTATTGGCAGCGGTGGTGAAGATACAATCTCCAGCTGACAAGGC
  CTGCGACACCCCTGATAAGCCGGTGCAAGTGACAAAGGAAGTTGTGTCAATGGGAACAGGAACAAAATGC
  ATAGGACAGTCCAAAATGAGGAAGAACGGAGACATCCTCAATGATAGCCATGCTGAGGTCATAGCCAGAA
  GGAGTTTCCAAAGGTACCTTCTCCACCAACTCCAGTTGGCAGCCACCCTGAAAGAGGATAGCATCTTTGT
  CCCAGGAACTCAAAAAGGAGTGTGGAAACTTAGACGAGACCTCATTTTTGTGTTTTTCTCCAGCCATACA
  CCCTGTGGGGATGCCTCCATCATTCCGATGCTTGAGTTTGAAGATCAGCCTTGCTGTCCTGTCTTCAGAA
  ATTGGGCCCACAACTCATCAGTAGAAGCCAGTAGTAACCTGGAAGCTCCTGGAAATGAAAGAAAATGTGA
  AGACCCTGACAGTCCTGTAACCAAAAAGATGAGGCTTGAGCCTGGGACTGCAGCCAGGGAGGTCACCAAC
  GGAGCAGCTCACCATCAGAGTTTTGGCAAGCAGAAAAGTGGCCCAATCTCACCAGGCATCCACAGCTGTG
  ATCTCACTGTAGAGGGACTGGCTACTGTCACCAGAATAGCCCCTGGTAGTGCCAAAGTGATAGACGTTTA
  TAGAACTGGAGCCAAGTGTGTACCTGGAGAAGCTGGAGACTCCGGAAAGCCGGGTGCTGCGTTTCACCAG
  GTGGGGCTGCTCCGAGTGAAGCCAGGCCGTGGAGACAGAACACGCTCCATGTCCTGTAGTGACAAGATGG
  CCCGATGGAACGTCCTCGGATGCCAAGGGGCACTGTTGATGCACTTGCTGGAAGAGCCCATCTACCTGTC
  AGCTGTGGTCATTGGGAAGTGCCCATACAGCCAGGAAGCCATGCAGAGAGCACTGATTGGAAGGTGTCAG
  AATGTGTCTGCTTTACCAAAAGGCTTCGGAGTTCAAGAATTAAAAATACTGCAGTCAGATTTACTATTTG
  AACAGAGCCGCAGTGCGGTGCAGGCAAAAAGGGCTGATAGCCCAGGTCGACTTGTTCCTTGTGGGGCAGC
  CATCAGCTGGAGTGCAGTTCCTGAGCAGCCTTTGGATGTTACTGCCAATGGCTTTCCACAGGGAACAACA
  AAGAAAACAATTGGAAGCCTTCAGGCAAGATCCCAAATCAGCAAAGTGGAACTCTTCAGATCATTCCAGA
  AGCTGCTAAGCAGAATTGCAAGGGACAAGTGGCCACACTCCCTCAGGGTGCAGAAGCTGGATACCTACCA
  GGAGTACAAGGAGGCTGCGTCCTCTTACCAGGAAGCCTGGAGCACACTCCGGAAGCAGGTGTTTGGATCC
  TGGATCAGAAACCCACCGGATTATCACCAGTTCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201044 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MWTADEIAQLCYEHYGIRLPKKGKPEPNHEWTLLAAVVKIQSPADKACDTPDKPVQVTKEVVSMGTGTKC
  IGQSKMRKNGDILNDSHAEVIARRSFQRYLLHQLQLAATLKEDSIFVPGTQKGVWKLRRDLIFVFFSSHT
  PCGDASIIPMLEFEDQPCCPVFRNWAHNSSVEASSNLEAPGNERKCEDPDSPVTKKMRLEPGTAAREVTN
  GAAHHQSFGKQKSGPISPGIHSCDLTVEGLATVTRIAPGSAKVIDVYRTGAKCVPGEAGDSGKPGAAFHQ
  VGLLRVKPGRGDRTRSMSCSDKMARWNVLGCQGALLMHLLEEPIYLSAVVIGKCPYSQEAMQRALIGRCQ
  NVSALPKGFGVQELKILQSDLLFEQSRSAVQAKRADSPGRLVPCGAAISWSAVPEQPLDVTANGFPQGTT
  KKTIGSLQARSQISKVELFRSFQKLLSRIARDKWPHSLRVQKLDTYQEYKEAASSYQEAWSTLRKQVFGS
  WIRNPPDYHQFK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012091
• ORF Size: 1506 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012091.5
• RefSeq Size: 3545 bp
• RefSeq ORF: 1509 bp
• Locus ID: 23536
• UniProt ID: Q9BUB4
• Cytogenetics: 16q23.1
• Domains: A_deamin
• MW: 55.4 kDa
• Gene Summary: This gene is a member of the ADAR (adenosine deaminase acting on RNA) family. Using site-specific adenosine modification, proteins encoded by these genes participate in the pre-mRNA editing of nuclear transcripts. The protein encoded by this gene, tRNA-specific adenosine deaminase 1, is responsible for the deamination of adenosine 37 to inosine in eukaryotic tRNA. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2010]