TAOK3 (NM_016281) Human Tagged ORF Clone

CAT#: RC201031

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TAOK3 (Myc-DDK-tagged)-Human TAO kinase 3 (TAOK3)



  "NM_016281" in other vectors (7)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 851.00


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Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol TAOK3
Synonyms DPK; hKFC-A; JIK; MAP3K18
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC201031 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGTAAAGGGGTGCTGAAGGACCCAGAGATTGCCGATCTATTCTACAAAGATGATCCTGAGGAACTTT
TTATTGGTTTGCATGAAATTGGACATGGAAGTTTTGGAGCAGTTTATTTTGCTACAAATGCTCACACCAG
TGAGGTGGTGGCAATTAAGAAGATGTCCTATAGTGGGAAGCAGACCCATGAGAAATGGCAAGATATTCTT
AAGGAAGTTAAATTTTTACGACAATTGAAGCATCCTAATACTATTGAGTACAAAGGCTGTTACTTGAAAG
AACACACTGCTTGGTTGGTGATGGAATATTGCTTAGGCTCAGCCTCTGATTTATTAGAAGTTCATAAAAA
ACCACTTCAGGAAGTGGAGATCGCTGCCATTACTCATGGAGCCTTGCATGGACTAGCCTACCTACATTCT
CATGCATTGATTCATAGGGATATTAAAGCAGGAAATATTCTTCTAACAGAGCCAGGTCAGGTAAAACTAG
CTGATTTTGGATCTGCTTCAATGGCTTCTCCTGCCAACTCCTTCGTGGGCACACCTTACTGGATGGCTCC
AGAGGTGATCTTAGCTATGGATGAAGGACAGTATGATGGGAAAGTTGATATTTGGTCACTTGGCATCACT
TGTATTGAATTGGCGGAACGGAAGCCGCCCCTTTTCAACATGAATGCAATGAGTGCCTTATATCACATTG
CCCAGAATGACTCCCCAACGTTACAGTCTAATGAATGGACAGACTCCTTTAGGAGATTTGTTGATTACTG
CTTGCAGAAAATACCTCAGGAAAGGCCAACATCAGCAGAACTATTAAGGCATGACTTTGTTCGACGAGAC
CGGCCACTACGTGTCCTCATTGACCTCATACAGAGGACAAAAGATGCAGTTCGTGAGCTAGATAACCTAC
AGTACCGAAAAATGAAAAAAATACTTTTCCAAGAGACACGGAATGGACCCTTGAATGAGTCACAGGAGGA
TGAGGAAGACAGTGAACATGGAACCAGCCTGAACAGGGAAATGGACAGCCTGGGCAGCAACCATTCCATT
CCAAGCATGTCCGTGAGCACAGGCAGCCAGAGCAGCAGTGTGAACAGCATGCAGGAAGTCATGGACGAGA
GCAGTTCCGAACTTGTCATGATGCACGATGACGAAAGCACAATCAATTCCAGCTCCTCCGTCGTGCATAA
GAAAGATCATGTATTCATAAGGGATGAGGCGGGCCACGGCGATCCCAGGCCTGAGCCGCGGCCTACCCAG
TCAGTTCAGAGCCAGGCCCTCCACTACCGGAACAGAGAGCGCTTTGCCACGATCAAATCAGCATCTTTGG
TTACACGACAGATCCATGAGCATGAGCAGGAGAACGAGTTGCGGGAACAGATGTCAGGTTATAAGCGGAT
GCGGCGCCAGCACCAGAAGCAGCTGATCGCCCTGGAGAACAAGCTGAAGGCTGAGATGGACGAGCACCGC
CTCAAGCTACAGAAGGAGGTGGAGACGCATGCCAACAACTCGTCCATCGAGCTGGAGAAGCTGGCCAAGA
AGCAAGTGGCTATCATAGAAAAGGAGGCAAAGGTAGCTGCAGCAGATGAGAAGAAGTTCCAGCAACAGAT
CTTGGCCCAGCAGAAGAAAGATTTGACAACTTTCTTAGAAAGTCAGAAGAAGCAGTATAAGATTTGTAAG
GAAAAAATAAAAGAGGAAATGAATGAGGACCATAGCACACCCAAGAAAGAGAAGCAAGAGCGGATCTCCA
AACATAAAGAGAACTTGCAGCACACACAGGCTGAAGAGGAAGCCCACCTTCTCACTCAACAGAGACTGTA
CTACGACAAAAATTGTCGTTTCTTCAAGCGGAAAATAATGATCAAGCGGCACGAGGTGGAGCAGCAGAAC
ATTCGGGAGGAACTAAATAAAAAGAGGACCCAGAAGGAGATGGAGCATGCCATGCTAATCCGGCACGACG
AGTCCACCCGAGAGCTAGAGTACAGGCAGCTGCACACGTTACAGAAGCTACGCATGGATCTGATCCGTTT
ACAGCACCAGACGGAACTGGAAAACCAGCTGGAGTACAATAAGAGGCGAGAAAGAGAACTGCACAGAAAG
CATGTCATGGAACTTCGGCAACAGCCAAAAAACTTAAAGGCCATGGAAATGCAAATTAAAAAACAGTTTC
AGGACACTTGCAAAGTACAGACCAAACAGTATAAAGCACTCAAGAATCACCAGTTGGAAGTTACTCCAAA
GAATGAGCACAAAACAATCTTAAAGACACTGAAAGATGAGCAGACAAGAAAACTTGCCATTTTGGCAGAG
CAGTATGAACAGAGTATAAATGAAATGATGGCCTCTCAAGCGTTACGGCTAGATGAGGCTCAAGAAGCAG
AATGCCAGGCCTTGAGGCTACAGCTCCAGCAGGAAATGGAGCTGCTCAACGCCTACCAGAGCAAAATCAA
GATGCAAACAGAGGCACAACATGAACGTGAGCTCCAGAAGCTAGAGCAGAGAGTGTCTCTGCGCAGAGCA
CACCTTGAGCAGAAGATTGAAGAGGAGCTGGCTGCCCTTCAGAAGGAACGCAGCGAGAGAATAAAGAACC
TATTGGAAAGGCAAGAGCGAGAGATTGAAACTTTTGACATGGAGAGCCTCAGAATGGGATTTGGGAATTT
GGTTACATTAGATTTTCCTAAGGAGGACTACAGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC201031 protein sequence
Red=Cloning site Green=Tags(s)

MRKGVLKDPEIADLFYKDDPEELFIGLHEIGHGSFGAVYFATNAHTSEVVAIKKMSYSGKQTHEKWQDIL
KEVKFLRQLKHPNTIEYKGCYLKEHTAWLVMEYCLGSASDLLEVHKKPLQEVEIAAITHGALHGLAYLHS
HALIHRDIKAGNILLTEPGQVKLADFGSASMASPANSFVGTPYWMAPEVILAMDEGQYDGKVDIWSLGIT
CIELAERKPPLFNMNAMSALYHIAQNDSPTLQSNEWTDSFRRFVDYCLQKIPQERPTSAELLRHDFVRRD
RPLRVLIDLIQRTKDAVRELDNLQYRKMKKILFQETRNGPLNESQEDEEDSEHGTSLNREMDSLGSNHSI
PSMSVSTGSQSSSVNSMQEVMDESSSELVMMHDDESTINSSSSVVHKKDHVFIRDEAGHGDPRPEPRPTQ
SVQSQALHYRNRERFATIKSASLVTRQIHEHEQENELREQMSGYKRMRRQHQKQLIALENKLKAEMDEHR
LKLQKEVETHANNSSIELEKLAKKQVAIIEKEAKVAAADEKKFQQQILAQQKKDLTTFLESQKKQYKICK
EKIKEEMNEDHSTPKKEKQERISKHKENLQHTQAEEEAHLLTQQRLYYDKNCRFFKRKIMIKRHEVEQQN
IREELNKKRTQKEMEHAMLIRHDESTRELEYRQLHTLQKLRMDLIRLQHQTELENQLEYNKRRERELHRK
HVMELRQQPKNLKAMEMQIKKQFQDTCKVQTKQYKALKNHQLEVTPKNEHKTILKTLKDEQTRKLAILAE
QYEQSINEMMASQALRLDEAQEAECQALRLQLQQEMELLNAYQSKIKMQTEAQHERELQKLEQRVSLRRA
HLEQKIEEELAALQKERSERIKNLLERQEREIETFDMESLRMGFGNLVTLDFPKEDYR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_016281
ORF Size 2694 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_016281.4
RefSeq Size 4399 bp
RefSeq ORF 2697 bp
Locus ID 51347
UniProt ID Q9H2K8
Cytogenetics 12q24.23
Domains pkinase, TyrKc, S_TKc
Protein Families Druggable Genome, Protein Kinase
Protein Pathways MAPK signaling pathway
MW 105.4 kDa
Gene Summary The protein encoded by this gene is a serine/threonine protein kinase that activates the p38/MAPK14 stress-activated MAPK cascade but inhibits the basal activity of the MAPK8/JNK cascade. The encoded protein is a member of the GCK subfamily of STE20-like kinases. [provided by RefSeq, Oct 2016]

Other Versions

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