WDR18 (NM_024100) Human Tagged ORF Clone

CAT#: RC201008

WDR18 (Myc-DDK-tagged)-Human WD repeat domain 18 (WDR18)

Product Images

Circular map for RC201008

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  Western blot validation of overexpression lysate (Cat# LY411371) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201008 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified WDR18 protein (Cat# TP301008). The protein was produced from HEK293T cells transfected with WDR18 cDNA clone (Cat# RC201008) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: WDR18
• Synonyms: Ipi3; R32184_1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201008 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGCCCATGGAGGTGGCCGTGTGTACGGACTCGGCGGCCCCGATGTGGAGCTGCATCGTGTGGG
  AACTTCACTCGGGCGCCAACCTGCTCACCTACCGCGGCGGCCAGGCGGGACCCCGCGGCCTGGCGCTGCT
  CAATGGCGAGTATCTGCTGGCGGCGCAGCTGGGCAAGAACTACATCAGCGCCTGGGAGCTGCAGCGGAAG
  GACCAGCTCCAGCAGAAGATCATGTGCCCCGGGCCTGTCACCTGTCTGACTGCATCACCCAATGGTCTCT
  ACGTCCTGGCAGGAGTTGCAGAAAGCATCCACCTGTGGGAGGTCTCCACCGGGAACCTTCTGGTCATCCT
  GAGTCGACACTACCAGGACGTCTCCTGCCTTCAGTTCACAGGGGACAGCAGCCACTTCATCTCAGGGGGC
  AAGGACTGCCTGGTGCTGGTTTGGAGCCTCTGCAGCGTGCTGCAGGCCGACCCCTCCAGGATTCCGGCGC
  CCAGGCACGTCTGGTCTCACCACACGCTCCCCATCACGGACCTGCACTGCGGCTTTGGGGGCCCCCTGGC
  CCGGGTGGCCACCTCCTCACTGGACCAGACGGTGAAGCTATGGGAGGTCTCCTCGGGGGAGCTGCTGCTC
  TCCGTCCTCTTTGACGTGTCCATCATGGCAGTGACCATGGACCTGGCTGAGCACCATATGTTCTGCGGGG
  GCAGTGAGGGCTCCATCTTCCAGGTCGACCTCTTCACCTGGCCCGGACAGAGGGAGAGGAGCTTCCACCC
  AGAGCAGGACGCCGGGAAGGTCTTCAAAGGGCACAGGAACCAGGTGACTTGCCTGTCAGTGTCCACTGAC
  GGCAGCGTGCTGCTCTCAGGCTCCCACGACGAGACCGTGCGCCTCTGGGACGTGCAGAGCAAGCAGTGCA
  TCCGGACGGTGGCCCTCAAAGGCCCAGTCACCAATGCCGCCATCCTGCTGGCGCCCGTCAGCATGCTGAG
  CTCAGACTTCAGGCCCAGCCTGCCGCTGCCCCACTTCAACAAGCACCTGCTGGGCGCCGAGCACGGGGAC
  GAGCCGCGCCACGGGGGCCTCACTCTGCGCCTGGGCCTCCACCAGCAGGGCTCGGAGCCCAGCTACCTGG
  ACCGCACGGAGCAGCTGCAGGCCGTCCTGTGCAGCACCATGGAGAAGAGCGTGCTCGGCGGCCAGGACCA
  GCTGCGCGTCCGTGTGACGGAGCTGGAGGACGAGGTGCGCAACCTGCGCAAGATCAATCGGGACCTGTTC
  GACTTCTCCACGCGCTTCATCACGCGGCCGGCCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201008 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAPMEVAVCTDSAAPMWSCIVWELHSGANLLTYRGGQAGPRGLALLNGEYLLAAQLGKNYISAWELQRK
  DQLQQKIMCPGPVTCLTASPNGLYVLAGVAESIHLWEVSTGNLLVILSRHYQDVSCLQFTGDSSHFISGG
  KDCLVLVWSLCSVLQADPSRIPAPRHVWSHHTLPITDLHCGFGGPLARVATSSLDQTVKLWEVSSGELLL
  SVLFDVSIMAVTMDLAEHHMFCGGSEGSIFQVDLFTWPGQRERSFHPEQDAGKVFKGHRNQVTCLSVSTD
  GSVLLSGSHDETVRLWDVQSKQCIRTVALKGPVTNAAILLAPVSMLSSDFRPSLPLPHFNKHLLGAEHGD
  EPRHGGLTLRLGLHQQGSEPSYLDRTEQLQAVLCSTMEKSVLGGQDQLRVRVTELEDEVRNLRKINRDLF
  DFSTRFITRPAK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024100
• ORF Size: 1296 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024100.4
• RefSeq Size: 1551 bp
• RefSeq ORF: 1299 bp
• Locus ID: 57418
• UniProt ID: Q9BV38
• Cytogenetics: 19p13.3
• MW: 47.4 kDa
• Gene Summary: This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. [provided by RefSeq, Jul 2008]