CDC37 (NM_007065) Human Tagged ORF Clone

CAT#: RC201002

CDC37 (Myc-DDK-tagged)-Human cell division cycle 37 homolog (S. cerevisiae) (CDC37)

Product Images

Western blot validation of overexpression lysate (Cat# LY416229) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201002 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified CDC37 protein (Cat# TP301002). The protein was produced from HEK293T cells transfected with CDC37 cDNA clone (Cat# RC201002) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CDC37
• Synonyms: P50CDC37
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201002 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGACTACAGCGTGTGGGACCACATTGAGGTGTCTGATGATGAAGACGAGACGCACCCCAACATCG
  ACACGGCCAGTCTCTTCCGCTGGCGGCATCAGGCCCGGGTGGAACGCATGGAGCAGTTCCAGAAGGAGAA
  GGAGGAACTGGACAGGGGCTGCCGCGAGTGCAAGCGCAAGGTGGCCGAGTGCCAGAGGAAACTGAAGGAG
  CTGGAGGTGGCCGAGGGCGGCAAGGCAGAGCTGGAGCGCCTGCAGGCCGAGGCACAGCAGCTGCGCAAGG
  AGGAGCGGAGCTGGGAGCAGAAGCTGGAGGAGATGCGCAAGAAGGAGAAGAGCATGCCCTGGAACGTGGA
  CACGCTCAGCAAAGACGGCTTCAGCAAGAGCATGGTAAATACCAAGCCCGAGAAGACGGAGGAGGACTCA
  GAGGAGGTGAGGGAGCAGAAACACAAGACCTTCGTGGAAAAATACGAGAAACAGATCAAGCACTTTGGCA
  TGCTTCGCCGCTGGGATGACAGCCAAAAGTACCTGTCAGACAACGTCCACCTGGTGTGCGAGGAGACAGC
  CAATTACCTGGTCATTTGGTGCATTGACCTAGAGGTGGAGGAGAAATGTGCACTCATGGAGCAGGTGGCC
  CACCAGACAATCGTCATGCAATTTATCCTGGAGCTGGCCAAGAGCCTAAAGGTGGACCCCCGGGCCTGCT
  TCCGGCAGTTCTTCACTAAGATTAAGACAGCCGATCGCCAGTACATGGAGGGCTTCAACGACGAGCTGGA
  AGCCTTCAAGGAGCGTGTGCGGGGCCGTGCCAAGCTGCGCATCGAGAAGGCCATGAAGGAGTACGAGGAG
  GAGGAGCGCAAGAAGCGGCTCGGCCCCGGCGGCCTGGACCCCGTCGAGGTCTACGAGTCCCTCCCTGAGG
  AACTCCAGAAGTGCTTCGATGTGAAGGACGTGCAGATGCTGCAGGACGCCATCAGCAAGATGGACCCCAC
  CGACGCAAAGTACCACATGCAGCGCTGCATTGACTCTGGCCTCTGGGTCCCCAACTCTAAGGCCAGCGAG
  GCCAAGGAGGGAGAGGAGGCAGGTCCTGGGGACCCATTACTGGAAGCTGTTCCCAAGACGGGCGATGAGA
  AGGATGTCAGTGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201002 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVDYSVWDHIEVSDDEDETHPNIDTASLFRWRHQARVERMEQFQKEKEELDRGCRECKRKVAECQRKLKE
  LEVAEGGKAELERLQAEAQQLRKEERSWEQKLEEMRKKEKSMPWNVDTLSKDGFSKSMVNTKPEKTEEDS
  EEVREQKHKTFVEKYEKQIKHFGMLRRWDDSQKYLSDNVHLVCEETANYLVIWCIDLEVEEKCALMEQVA
  HQTIVMQFILELAKSLKVDPRACFRQFFTKIKTADRQYMEGFNDELEAFKERVRGRAKLRIEKAMKEYEE
  EERKKRLGPGGLDPVEVYESLPEELQKCFDVKDVQMLQDAISKMDPTDAKYHMQRCIDSGLWVPNSKASE
  AKEGEEAGPGDPLLEAVPKTGDEKDVSV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007065
• ORF Size: 1134 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007065.4
• RefSeq Size: 1693 bp
• RefSeq ORF: 1137 bp
• Locus ID: 11140
• UniProt ID: Q16543
• Cytogenetics: 19p13.2
• Domains: Cdc37
• MW: 44.5 kDa
• Gene Summary: The protein encoded by this gene is highly similar to Cdc 37, a cell division cycle control protein of Sacchromyces cerevisiae. This protein is a molecular chaperone with specific function in cell signal transduction. It has been shown to form complex with Hsp90 and a variety of protein kinases including CDK4, CDK6, SRC, RAF-1, MOK, as well as eIF2 alpha kinases. It is thought to play a critical role in directing Hsp90 to its target kinases. [provided by RefSeq, Jul 2008]