GDAP1L1 (NM_024034) Human Tagged ORF Clone

CAT#: RC200976

GDAP1L1 (Myc-DDK-tagged)-Human ganglioside-induced differentiation-associated protein 1-like 1 (GDAP1L1)

Product Images

Circular map for RC200976

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY GDAP1L1 (Cat# RC200976, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GDAP1L1(Cat# TA503153). Positive lysates LY411411 (100ug) and LC411411 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY411411) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200976 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified GDAP1L1 protein (Cat# TP300976). The protein was produced from HEK293T cells transfected with GDAP1L1 cDNA clone (Cat# RC200976) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GDAP1L1
• Synonyms: dJ881L22.1; dJ995J12.1.1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200976 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGACCCCCAACAATCTGACCCCCACCAACTGCAGCTGGTGGCCCATCTCCGCGCTGGAGAGCGATG
  CGGCCAAGCCAGCGGAGGCCCCCGACGCTCCCGAGGCGGCCAGCCCCGCCCATTGGCCCAGGGAGAGCCT
  GGTTCTGTACCACTGGACCCAGTCCTTCAGCTCGCAGAAGGTGCGGCTGGTGATCGCCGAGAAGGGCCTG
  GTGTGCGAGGAGCGGGACGTGAGCCTGCCACAGAGCGAGCACAAGGAGCCCTGGTTCATGCGGCTCAACC
  TGGGCGAGGAGGTGCCCGTCATCATCCACCGCGACAACATCATCAGTGACTATGACCAGATCATTGACTA
  TGTGGAGCGCACCTTCACAGGAGAGCACGTGGTGGCCCTGATGCCCGAGGTGGGCAGCCTGCAGCACGCA
  CGGGTGCTGCAGTACCGGGAGCTGCTGGACGCACTGCCCATGGATGCCTACACGCATGGCTGCATCCTGC
  ATCCCGAGCTCACCACCGACTCCATGATCCCCAAGTACGCCACGGCCGAGATCCGCAGACATTTAGCCAA
  TGCCACCACGGACCTCATGAAACTGGACCATGAAGAGGAGCCCCAGCTCTCCGAGCCCTACCTTTCTAAA
  CAAAAGAAGCTCATGGCCAAGATCTTGGAGCATGATGATGTGAGCTACCTGAAGAAGATCCTCGGGGAAC
  TGGCCATGGTGCTGGACCAGATTGAGGCGGAGCTGGAGAAGAGGAAGCTGGAGAACGAGGGGCAGAAATG
  CGAGCTGTGGCTCTGTGGCTGTGCCTTCACCCTCGCTGATGTCCTCCTGGGAGCCACCCTGCACCGCCTC
  AAGTTCCTGGGACTGTCCAAGAAATACTGGGAAGATGGCAGCCGGCCCAACCTGCAGTCCTTCTTTGAGA
  GGGTCCAGAGACGCTTTGCCTTCCGGAAAGTCCTGGGTGACATCCACACCACCCTGCTGTCGGCCGTCAT
  CCCCAATGCTTTCCGGCTGGTCAAGAGGAAACCCCCATCCTTCTTCGGGGCGTCCTTCCTCATGGGCTCC
  CTGGGTGGGATGGGCTACTTTGCCTACTGGTACCTCAAGAAAAAATACATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200976 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MATPNNLTPTNCSWWPISALESDAAKPAEAPDAPEAASPAHWPRESLVLYHWTQSFSSQKVRLVIAEKGL
  VCEERDVSLPQSEHKEPWFMRLNLGEEVPVIIHRDNIISDYDQIIDYVERTFTGEHVVALMPEVGSLQHA
  RVLQYRELLDALPMDAYTHGCILHPELTTDSMIPKYATAEIRRHLANATTDLMKLDHEEEPQLSEPYLSK
  QKKLMAKILEHDDVSYLKKILGELAMVLDQIEAELEKRKLENEGQKCELWLCGCAFTLADVLLGATLHRL
  KFLGLSKKYWEDGSRPNLQSFFERVQRRFAFRKVLGDIHTTLLSAVIPNAFRLVKRKPPSFFGASFLMGS
  LGGMGYFAYWYLKKKYI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024034
• ORF Size: 1101 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024034.6
• RefSeq Size: 2798 bp
• RefSeq ORF: 1104 bp
• Locus ID: 78997
• UniProt ID: Q96MZ0
• Cytogenetics: 20q13.12
• Protein Families: Transmembrane
• MW: 42 kDa
• Gene Summary: The ganglioside GD3 synthase causes cell differentiation with neurite sprouting when transfected into the mouse neuroblastoma cell line Neuro2a. After differentiation, the expression of several genes is upregulated, including one that encodes a protein termed ganglioside-induced differentiation-associated protein 1 (Gdap1). A similar gene was found in humans, and mutations in the human gene are associated with Charcot-Marie-Tooth type 4A disease. The protein encoded by this gene is similar in sequence to the human GDAP1 protein. Several transcript variants encoding different isoforms, as well as a noncoding transcript variant, have been found for this gene. [provided by RefSeq, Feb 2012]