SFXN3 (NM_030971) Human Tagged ORF Clone

CAT#: RC200974

SFXN3 (Myc-DDK-tagged)-Human sideroflexin 3 (SFXN3)

Product Images

Western blot validation of overexpression lysate (Cat# LY410634) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200974 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified SFXN3 protein (Cat# TP300974). The protein was produced from HEK293T cells transfected with SFXN3 cDNA clone (Cat# RC200974) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SFXN3
• Synonyms: BA108L7.2; SFX3; SLC56A3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200974 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAAGCAAAATGGGTGAATTGCCTTTAGACATCAACATCCAGGAACCTCGCTGGGACCAAAGTACTT
  TCCTGGGCAGAGCCCGGCACTTTTTCACTGTTACTGATCCTCGAAATCTGCTGCTGTCCGGGGCACAGCT
  GGAAGCTTCTCGGAACATCGTGCAGAACTACAGGGCCGGCGTGGTGACCCCAGGGATCACCGAGGACCAG
  CTGTGGAGGGCCAAGTATGTGTATGACTCCGCCTTCCATCCGGACACAGGGGAGAAGGTGGTCCTGATTG
  GCCGCATGTCAGCCCAGGTGCCCATGAACATGACCATCACTGGCTGCATGCTCACATTCTACAGGAAGAC
  CCCAACCGTGGTGTTCTGGCAGTGGGTGAATCAGTCCTTCAATGCCATTGTTAACTACTCCAACCGCAGT
  GGTGACACTCCCATCACTGTGAGGCAGCTGGGGACAGCCTATGTGAGTGCCACCACTGGAGCTGTGGCCA
  CGGCCCTGGGACTCAAATCCCTCACCAAGCACCTGCCCCCCTTGGTCGGCAGATTTGTGCCCTTTGCAGC
  AGTGGCAGCTGCCAACTGCATCAACATCCCCCTGATGAGGCAGAGAGAGCTGCAGGTGGGCATCCCGGTG
  GCTGATGAGGCAGGTCAGAGGCTTGGCTACTCGGTGACTGCAGCCAAGCAGGGAATCTTCCAGGTGGTGA
  TTTCAAGAATCTGCATGGCGATTCCTGCCATGGCCATCCCACCACTGATCATGGACACTCTGGAGAAGAA
  AGACTTCCTGAAGCGCCGCCCCTGGCTGGGGGCACCCCTGCAGGTGGGACTGGTGGGCTTCTGCCTGGTA
  TTTGCAACCCCCCTGTGCTGTGCCCTATTCCCCCAGAAGAGCTCCATACACATAAGCAACCTGGAACCAG
  AGCTGAGAGCTCAGATCCATGAGCAAAACCCCAGCGTTGAAGTGGTCTACTACAACAAGGGGCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200974 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MESKMGELPLDINIQEPRWDQSTFLGRARHFFTVTDPRNLLLSGAQLEASRNIVQNYRAGVVTPGITEDQ
  LWRAKYVYDSAFHPDTGEKVVLIGRMSAQVPMNMTITGCMLTFYRKTPTVVFWQWVNQSFNAIVNYSNRS
  GDTPITVRQLGTAYVSATTGAVATALGLKSLTKHLPPLVGRFVPFAAVAAANCINIPLMRQRELQVGIPV
  ADEAGQRLGYSVTAAKQGIFQVVISRICMAIPAMAIPPLIMDTLEKKDFLKRRPWLGAPLQVGLVGFCLV
  FATPLCCALFPQKSSIHISNLEPELRAQIHEQNPSVEVVYYNKGL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_030971
• ORF Size: 975 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030971.3, NP_112233.2
• RefSeq Size: 3129 bp
• RefSeq ORF: 978 bp
• Locus ID: 81855
• UniProt ID: Q9BWM7
• Cytogenetics: 10q24.31
• Domains: Mtc
• Protein Families: Transmembrane
• MW: 36 kDa
• Gene Summary: Mitochondrial serine transporter that mediates transport of serine into mitochondria, an important step of the one-carbon metabolism pathway (PubMed:30442778). Mitochondrial serine is converted to glycine and formate, which then exits to the cytosol where it is used to generate the charged folates that serve as one-carbon donors (PubMed:30442778).[UniProtKB/Swiss-Prot Function]