SFXN3 (NM_030971) Human Tagged ORF Clone

CAT#: RC200974

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SFXN3 (Myc-DDK-tagged)-Human sideroflexin 3 (SFXN3)



  "NM_030971" in other vectors (4)

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 118.00

USD 517.00


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Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol SFXN3
Synonyms BA108L7.2; SFX3; SLC56A3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC200974 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAAGCAAAATGGGTGAATTGCCTTTAGACATCAACATCCAGGAACCTCGCTGGGACCAAAGTACTT
TCCTGGGCAGAGCCCGGCACTTTTTCACTGTTACTGATCCTCGAAATCTGCTGCTGTCCGGGGCACAGCT
GGAAGCTTCTCGGAACATCGTGCAGAACTACAGGGCCGGCGTGGTGACCCCAGGGATCACCGAGGACCAG
CTGTGGAGGGCCAAGTATGTGTATGACTCCGCCTTCCATCCGGACACAGGGGAGAAGGTGGTCCTGATTG
GCCGCATGTCAGCCCAGGTGCCCATGAACATGACCATCACTGGCTGCATGCTCACATTCTACAGGAAGAC
CCCAACCGTGGTGTTCTGGCAGTGGGTGAATCAGTCCTTCAATGCCATTGTTAACTACTCCAACCGCAGT
GGTGACACTCCCATCACTGTGAGGCAGCTGGGGACAGCCTATGTGAGTGCCACCACTGGAGCTGTGGCCA
CGGCCCTGGGACTCAAATCCCTCACCAAGCACCTGCCCCCCTTGGTCGGCAGATTTGTGCCCTTTGCAGC
AGTGGCAGCTGCCAACTGCATCAACATCCCCCTGATGAGGCAGAGAGAGCTGCAGGTGGGCATCCCGGTG
GCTGATGAGGCAGGTCAGAGGCTTGGCTACTCGGTGACTGCAGCCAAGCAGGGAATCTTCCAGGTGGTGA
TTTCAAGAATCTGCATGGCGATTCCTGCCATGGCCATCCCACCACTGATCATGGACACTCTGGAGAAGAA
AGACTTCCTGAAGCGCCGCCCCTGGCTGGGGGCACCCCTGCAGGTGGGACTGGTGGGCTTCTGCCTGGTA
TTTGCAACCCCCCTGTGCTGTGCCCTATTCCCCCAGAAGAGCTCCATACACATAAGCAACCTGGAACCAG
AGCTGAGAGCTCAGATCCATGAGCAAAACCCCAGCGTTGAAGTGGTCTACTACAACAAGGGGCTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC200974 protein sequence
Red=Cloning site Green=Tags(s)

MESKMGELPLDINIQEPRWDQSTFLGRARHFFTVTDPRNLLLSGAQLEASRNIVQNYRAGVVTPGITEDQ
LWRAKYVYDSAFHPDTGEKVVLIGRMSAQVPMNMTITGCMLTFYRKTPTVVFWQWVNQSFNAIVNYSNRS
GDTPITVRQLGTAYVSATTGAVATALGLKSLTKHLPPLVGRFVPFAAVAAANCINIPLMRQRELQVGIPV
ADEAGQRLGYSVTAAKQGIFQVVISRICMAIPAMAIPPLIMDTLEKKDFLKRRPWLGAPLQVGLVGFCLV
FATPLCCALFPQKSSIHISNLEPELRAQIHEQNPSVEVVYYNKGL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_030971
ORF Size 975 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_030971.3, NP_112233.2
RefSeq Size 3129 bp
RefSeq ORF 978 bp
Locus ID 81855
UniProt ID Q9BWM7
Cytogenetics 10q24.31
Domains Mtc
Protein Families Transmembrane
MW 36 kDa
Gene Summary Mitochondrial serine transporter that mediates transport of serine into mitochondria, an important step of the one-carbon metabolism pathway (PubMed:30442778). Mitochondrial serine is converted to glycine and formate, which then exits to the cytosol where it is used to generate the charged folates that serve as one-carbon donors (PubMed:30442778).[UniProtKB/Swiss-Prot Function]

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