NUDT21 (NM_007006) Human Tagged ORF Clone
CAT#: RC200936
NUDT21 (Myc-DDK-tagged)-Human nudix (nucleoside diphosphate linked moiety X)-type motif 21 (NUDT21)
ORF Plasmid: tGFP
Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro
AAV Particle: DDK
"NM_007006" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | NUDT21 |
Synonyms | CFIM25; CPSF5 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC200936 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGTGGTACCGCCCAATCGCTCGCAGACCGGCTGGCCCCGGGGGGTCACTCAGTTCGGCAACAAGT ACATCCAGCAGACGAAGCCCCTCACCCTGGAGCGCACCATCAACCTGTACCCTCTTACCAATTATACTTT TGGTACAAAAGAGCCCCTCTACGAGAAGGACAGCTCTGTTGCAGCCAGATTTCAGCGCATGAGGGAAGAA TTTGATAAAATTGGAATGAGGAGGACTGTAGAAGGGGTTCTGATTGTACATGAGCACCGGCTACCCCATG TGTTACTGCTGCAGCTGGGAACAACTTTCTTCAAACTACCTGGTGGTGAACTTAACCCAGGAGAAGATGA AGTTGAAGGACTAAAACGCTTAATGACAGAGATACTGGGTCGTCAGGATGGAGTTTTGCAAGACTGGGTC ATTGACGATTGCATTGGTAACTGGTGGAGACCAAATTTTGAACCTCCTCAGTATCCATATATTCCTGCAC ATATTACAAAGCCTAAGGAACATAAGAAGTTGTTTCTGGTTCAGCTTCAAGAAAAAGCCTTGTTTGCAGT CCCTAAAAATTACAAGCTGGTAGCTGCACCATTGTTTGAATTGTATGACAATGCACCAGGATATGGACCC ATCATTTCTAGTCTCCCTCAGCTGTTGAGCAGGTTCAATTTTATTTACAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200936 protein sequence
Red=Cloning site Green=Tags(s) MSVVPPNRSQTGWPRGVTQFGNKYIQQTKPLTLERTINLYPLTNYTFGTKEPLYEKDSSVAARFQRMREE FDKIGMRRTVEGVLIVHEHRLPHVLLLQLGTTFFKLPGGELNPGEDEVEGLKRLMTEILGRQDGVLQDWV IDDCIGNWWRPNFEPPQYPYIPAHITKPKEHKKLFLVQLQEKALFAVPKNYKLVAAPLFELYDNAPGYGP IISSLPQLLSRFNFIYN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_007006 |
ORF Size | 681 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_007006.3 |
RefSeq Size | 4407 bp |
RefSeq ORF | 684 bp |
Locus ID | 11051 |
UniProt ID | O43809 |
Cytogenetics | 16q13 |
Domains | NUDIX |
MW | 26.2 kDa |
Gene Summary | The protein encoded by this gene is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of the protein with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors. This gene encodes the 25kD subunit of the protein complex, which is composed of four polypeptides. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC200936L1 | Lenti ORF clone of Human nudix (nucleoside diphosphate linked moiety X)-type motif 21 (NUDT21), Myc-DDK-tagged |
USD 600.00 |
|
RC200936L2 | Lenti ORF clone of Human nudix (nucleoside diphosphate linked moiety X)-type motif 21 (NUDT21), mGFP tagged |
USD 600.00 |
|
RC200936L3 | Lenti ORF clone of Human nudix (nucleoside diphosphate linked moiety X)-type motif 21 (NUDT21), Myc-DDK-tagged |
USD 600.00 |
|
RC200936L4 | Lenti ORF clone of Human nudix (nucleoside diphosphate linked moiety X)-type motif 21 (NUDT21), mGFP tagged |
USD 600.00 |
|
RG200936 | NUDT21 (tGFP-tagged) - Human nudix (nucleoside diphosphate linked moiety X)-type motif 21 (NUDT21) |
USD 500.00 |
|
SC115739 | NUDT21 (untagged)-Human nudix (nucleoside diphosphate linked moiety X)-type motif 21 (NUDT21) |
USD 300.00 |
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