MOV10 (NM_020963) Human Tagged ORF Clone

CAT#: RC200934

MOV10 (Myc-DDK-tagged)-Human Mov10, Moloney leukemia virus 10, homolog (mouse) (MOV10), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY427147) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC226296 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified MOV10 protein (Cat# TP300934). The protein was produced from HEK293T cells transfected with MOV10 cDNA clone (Cat# RC200934) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MOV10
• Synonyms: fSAP113; gb110
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200934 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCAGTAAGTTCAGCTGCCGGCAGCTCCGGGAGGCGGGCCAGTGTTTCGAGAGTTTCCTGGTCGTTC
  GGGGACTGGACATGGAGACAGATCGCGAGCGGCTGCGGACCATTTATAACCGCGACTTCAAGATCAGCTT
  TGGGACCCCCGCCCCTGGCTTCTCCTCCATGCTGTATGGAATGAAGATTGCAAATCTGGCCTACGTCACC
  AAGACTCGGGTCAGGTTCTTCAGACTCGACCGCTGGGCCGACGTGCGGTTCCCAGAAAAGAGGAGAATGA
  AGCTGGGGTCAGATATCAGCAAACACCACAAGTCACTGCTAGCCAAGATCTTTTATGACAGGGCTGAGTA
  TCTTCATGGGAAACATGGTGTGGATGTGGAAGTCCAGGGGCCCCATGAAGCCCGAGATGGGCAGCTCCTT
  ATCCGCCTGGATTTGAACCGCAAAGAGGTGCTGACCCTGAGGCTTCGGAATGGCGGAACCCAGTCTGTTA
  CCCTCACTCACCTCTTCCCACTCTGCCGGACACCCCAGTTTGCTTTCTACAATGAAGACCAGGAGTTGCC
  CTGTCCACTGGGCCCCGGTGAATGCTATGAACTCCATGTCCATTGTAAGACCAGCTTTGTGGGCTACTTC
  CCAGCCACAGTGCTCTGGGAGCTGCTGGGACCTGGGGAGTCGGGTTCAGAAGGAGCCGGCACATTCTACA
  TTGCCCGCTTCTTGGCTGCCGTCGCCCACAGCCCCCTGGCTGCACAGCTGAAGCCCATGACTCCCTTCAA
  GCGGACCCGGATCACCGGAAACCCTGTGGTGACCAATCGGATAGAGGAAGGAGAGAGACCTGACCGCGCT
  AAGGGCTATGACCTGGAGTTAAGTATGGCGCTGGGGACATACTACCCACCTCCCCGCCTCAGGCAGCTGC
  TCCCCATGCTTCTTCAGGGAACAAGTATCTTCACTGCCCCTAAGGAGATCGCAGAGATCAAGGCCCAGCT
  GGAGACAGCCCTGAAGTGGAGGAACTATGAGGTGAAGCTGCGGCTGCTGCTGCACCTGGAGGAACTGCAG
  ATGGAGCATGATATCCGGCACTATGACCTGGAGTCGGTGCCCATGACCTGGGACCCTGTGGACCAGAACC
  CCAGGCTGCTCACGCTGGAGGTTCCTGGAGTGACTGAGAGCCGCCCCTCAGTGCTACGGGGCGACCACCT
  GTTTGCCCTTTTGTCCTCGGAGACACACCAGGAGGACCCCATCACATATAAGGGCTTTGTGCACAAGGTG
  GAATTGGACCGTGTCAAGCTGAGCTTTTCCATGAGCCTCCTGAGCCGCTTTGTGGATGGGCTGACCTTCA
  AGGTGAACTTTACCTTCAACCGCCAGCCGCTGCGAGTCCAGCACCGTGCCCTGGAGCTGACAGGGCGCTG
  GCTGCTGTGGCCCATGCTCTTTCCTGTGGCACCTCGGGACGTCCCGCTGCTGCCCTCAGATGTGAAACTC
  AAGCTGTACGACCGGAGTCTGGAGTCAAACCCAGAGCAGCTGCAGGCCATGAGGCACATTGTTACGGGCA
  CCACCCGTCCAGCCCCCTACATCATCTTTGGGCCTCCAGGCACCGGCAAGACTGTCACGTTAGTGGAGGC
  AATTAAGCAGGTGGTGAAGCACTTGCCCAAAGCCCACATCTTGGCCTGCGCTCCATCCAACTCAGGGGCT
  GACCTACTCTGTCAAAGGCTCCGGGTCCACCTTCCTAGCTCCATCTACCGCCTCCTGGCCCCCAGCAGGG
  ACATCCGCATGGTACCTGAGGACATCAAGCCCTGCTGCAACTGGGACGCAAAGAAGGGGGAGTATGTATT
  TCCCGCCAAGAAGAAGCTGCAGGAATACCGGGTCTTAATTACCACCCTCATCACTGCCGGCAGGTTGGTC
  TCGGCCCAGTTTCCCATTGATCACTTCACACACATCTTCATCGATGAGGCTGGCCACTGCATGGAGCCTG
  AGAGTCTGGTAGCTATAGCAGGGCTGATGGAAGTAAAGGAAACAGGTGATCCAGGAGGGCAGCTGGTGCT
  GGCAGGAGACCCTCGGCAGCTGGGGCCTGTGCTGCGTTCCCCACTGACCCAGAAGCATGGACTGGGATAC
  TCACTGCTGGAGCGGCTGCTCACCTACAACTCCCTGTACAAGAAGGGCCCTGATGGCTATGACCCCCAGT
  TCATAACCAAGCTGCTCCGCAACTACAGGTCTCATCCCACCATCCTGGACATTCCTAACCAGCTCTATTA
  TGAAGGGGAGCTGCAGGCCTGTGCTGATGTCGTGGATCGAGAACGCTTCTGCCGCTGGGCGGGCCTACCT
  CGACAGGGCTTTCCCATCATCTTTCACGGCGTAATGGGCAAAGATGAGCGTGAAGGCAACAGCCCATCCT
  TCTTCAACCCTGAAGAGGCTGCCACAGTGACTTCCTACCTGAAGCTGCTCCTGGCCCCCTCCTCCAAGAA
  GGGCAAAGCTCGCCTGAGCCCTCGAAGTGTGGGCGTCATCTCCCCGTACCGGAAACAGGTGGAGAAAATC
  CGTTACTGCATCACCAAACTTGACAGGGAGCTTCGAGGACTGGATGACATCAAGGACTTGAAGGTGGGTT
  CAGTAGAAGAATTCCAAGGCCAAGAACGAAGCGTCATCCTCATCTCCACCGTGCGAAGCAGCCAGAGCTT
  TGTGCAGCTGGATCTGGACTTTAATCTGGGTTTCCTTAAGAACCCCAAGAGGTTCAATGTAGCTGTGACC
  CGGGCCAAGGCCCTGCTCATCATCGTGGGGAACCCCCTTCTCCTGGGCCATGACCCTGACTGGAAAGTAT
  TCCTGGAGTTCTGTAAAGAAAACGGAGGGTATACCGGGTGTCCCTTCCCTGCCAAACTGGACCTGCAACA
  GGGACAGAATTTACTGCAAGGTCTGAGCAAGCTCAGCCCCTCTACCTCAGGGCCCCACAGCCATGACTAC
  CTCCCCCAGGAGCGGGAGGGTGAAGGGGGCCTGTCTCTGCAAGTGGAGCCAGAGTGGAGGAATGAGCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200934 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPSKFSCRQLREAGQCFESFLVVRGLDMETDRERLRTIYNRDFKISFGTPAPGFSSMLYGMKIANLAYVT
  KTRVRFFRLDRWADVRFPEKRRMKLGSDISKHHKSLLAKIFYDRAEYLHGKHGVDVEVQGPHEARDGQLL
  IRLDLNRKEVLTLRLRNGGTQSVTLTHLFPLCRTPQFAFYNEDQELPCPLGPGECYELHVHCKTSFVGYF
  PATVLWELLGPGESGSEGAGTFYIARFLAAVAHSPLAAQLKPMTPFKRTRITGNPVVTNRIEEGERPDRA
  KGYDLELSMALGTYYPPPRLRQLLPMLLQGTSIFTAPKEIAEIKAQLETALKWRNYEVKLRLLLHLEELQ
  MEHDIRHYDLESVPMTWDPVDQNPRLLTLEVPGVTESRPSVLRGDHLFALLSSETHQEDPITYKGFVHKV
  ELDRVKLSFSMSLLSRFVDGLTFKVNFTFNRQPLRVQHRALELTGRWLLWPMLFPVAPRDVPLLPSDVKL
  KLYDRSLESNPEQLQAMRHIVTGTTRPAPYIIFGPPGTGKTVTLVEAIKQVVKHLPKAHILACAPSNSGA
  DLLCQRLRVHLPSSIYRLLAPSRDIRMVPEDIKPCCNWDAKKGEYVFPAKKKLQEYRVLITTLITAGRLV
  SAQFPIDHFTHIFIDEAGHCMEPESLVAIAGLMEVKETGDPGGQLVLAGDPRQLGPVLRSPLTQKHGLGY
  SLLERLLTYNSLYKKGPDGYDPQFITKLLRNYRSHPTILDIPNQLYYEGELQACADVVDRERFCRWAGLP
  RQGFPIIFHGVMGKDEREGNSPSFFNPEEAATVTSYLKLLLAPSSKKGKARLSPRSVGVISPYRKQVEKI
  RYCITKLDRELRGLDDIKDLKVGSVEEFQGQERSVILISTVRSSQSFVQLDLDFNLGFLKNPKRFNVAVT
  RAKALLIIVGNPLLLGHDPDWKVFLEFCKENGGYTGCPFPAKLDLQQGQNLLQGLSKLSPSTSGPHSHDY
  LPQEREGEGGLSLQVEPEWRNEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020963
• ORF Size: 3009 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020963.4
• RefSeq Size: 3767 bp
• RefSeq ORF: 3012 bp
• Locus ID: 4343
• UniProt ID: Q9HCE1
• Cytogenetics: 1p13.2
• MW: 113.7 kDa
• Gene Summary: 5' to 3' RNA helicase contributing to UPF1 mRNA target degradation by translocation along 3' UTRs (PubMed:24726324). Required for microRNA (miRNA)-mediated gene silencing by the RNA-induced silencing complex (RISC). Required for both miRNA-mediated translational repression and miRNA-mediated cleavage of complementary mRNAs by RISC (PubMed:16289642, PubMed:17507929, PubMed:22791714). In cooperation with FMR1, regulates miRNA-mediated translational repression by AGO2 (PubMed:25464849). Restricts retrotransposition of long interspersed element-1 (LINE-1) in cooperation with TUT4 and TUT7 counteracting the RNA chaperonne activity of L1RE1 (PubMed:30122351, PubMed:23093941). Facilitates LINE-1 uridylation by TUT4 and TUT7 (PubMed:30122351). Required for embryonic viability and for normal central nervous system development and function. Plays two critical roles in early brain development: suppresses retroelements in the nucleus by directly inhibiting cDNA synthesis, while regulates cytoskeletal mRNAs to influence neurite outgrowth in the cytosol (By similarity). May function as a messenger ribonucleoprotein (mRNP) clearance factor (PubMed:24726324).[UniProtKB/Swiss-Prot Function]