TRIM56 (NM_030961) Human Tagged ORF Clone

CAT#: RC200891

TRIM56 (Myc-DDK-tagged)-Human tripartite motif containing 56 (TRIM56)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY TRIM56 (Cat# RC200891, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM56 (Cat# TA808895)(1:2000). Positive lysates LY410625 (100ug) and LC410625 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY410625) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200891 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TRIM56
• Synonyms: RNF109
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200891 representing NM_030961
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC
  GCCC
  
  ATGGTTTCCCACGGGTCCTCGCCCTCCCTCCTGGAGGCCCTGAGCAGCGACTTCCTGGCCTGTAAAATCT
  GCCTGGAGCAGCTGCGGGCACCCAAGACACTGCCCTGCCTGCATACCTACTGCCAAGACTGCCTGGCACA
  GCTGGCGGATGGCGGCCGCGTCCGCTGCCCCGAGTGCCGCGAGACAGTGCCTGTGCCGCCCGAGGGTGTG
  GCCTCCTTCAAGACCAACTTCTTCGTCAATGGGCTGCTGGACCTGGTGAAGGCCCGGGCCTGTGGAGACC
  TGCGTGCCGGGAAGCCAGCCTGTGCCCTGTGTCCCCTGGTGGGTGGCACCAGCACCGGGGGGCCGGCCAC
  GGCCCGGTGCCTGGACTGTGCCGATGACTTGTGCCAGGCCTGTGCCGACGGGCACCGCTGCACCCGCCAG
  ACCCACACCCACCGCGTGGTGGACCTGGTGGGCTACAGGGCCGGGTGGTATGATGAGGAGGCCCGGGAGC
  GCCAAGCGGCCCAGTGTCCCCAGCACCCCGGGGAGGCACTGCGCTTCCTGTGCCAGCCCTGCTCACAGTT
  GCTGTGCAGAGAGTGCCGCCTAGACCCCCACCTGGACCACCCCTGCCTGCCTCTGGCTGAAGCTGTGCGT
  GCCCGGAGGCCGGGCCTGGAGGGACTGCTGGCCGGTGTGGACAATAACCTGGTGGAGCTGGAGGCAGCGC
  GGAGGGTGGAGAAGGAGGCGCTAGCCCGGCTGCGGGAGCAGGCGGCCCGGGTGGGGACTCAGGTGGAGGA
  GGCGGCTGAGGGCGTCCTCCGGGCCCTGCTGGCCCAGAAGCAGGAGGTGCTGGGGCAGCTACGAGCCCAC
  GTGGAGGCTGCCGAAGAAGCTGCTCGGGAGAGGCTGGCGGAGCTTGAGGGCCGGGAGCAGGTGGCCAGGG
  CGGCAGCCGCCTTCGCCCGCCGGGTACTCAGCCTGGGGCGAGAGGCCGAGATCCTCTCCCTGGAAGGGGC
  GATCGCACAGCGGCTCAGGCAGCTGCAGGGCTGCCCCTGGGCACCAGGCCCGGCCCCCTGCCTGCTCCCA
  CAGCTGGAGCTCCATCCTGGGCTCCTGGACAAGAACTGCCACCTTCTTCGGCTGTCCTTTGAGGAGCAGC
  AGCCCCAGAAGGATGGTGGGAAAGACGGAGCTGGTACCCAGGGAGGTGAGGAGAGCCAGAGCCGGAGGGA
  GGATGAGCCGAAGACTGAGAGACAGGGTGGAGTCCAGCCCCAGGCCGGAGATGGAGCCCAGACCCCAAAA
  GAGGAAAAAGCCCAGACAACCCGAGAAGAGGGAGCCCAGACCTTGGAGGAGGACAGGGCCCAGACACCCC
  ACGAGGATGGAGGACCCCAGCCCCACAGGGGTGGCAGACCCAACAAGAAGAAAAAGTTCAAAGGCAGGCT
  CAAGTCAATTTCCCGGGAGCCCAGCCCAGCCCTGGGGCCGAATCTGGACGGCTCTGGCCTCCTCCCCAGA
  CCCATCTTTTACTGCAGTTTCCCCACGCGGATGCCTGGAGACAAGCGGTCCCCCCGGATCACCGGGCTCT
  GTCCCTTCGGTCCCCGGGAGATCCTGGTGGCGGATGAGCAGAACCGGGCACTGAAACGCTTCTCCCTCAA
  CGGCGACTACAAGGGCACCGTGCCGGTCCCTGAGGGCTGCTCCCCTTGCAGCGTGGCCGCCCTGCAGAGC
  GCGGTGGCCTTCTCCGCTAGCGCACGGCTCTATCTCATCAACCCCAATGGCGAAGTGCAGTGGCGCAGGG
  CCCTGAGCCTCTCCCAGGCCAGCCACGCGGTGGCGGCACTGCCTAGCGGGGACCGCGTGGCTGTCAGCGT
  GGCGGGCCACGTGGAGGTGTACAATATGGAAGGCAGCCTGGCCACCCGGTTCATTCCTGGAGGCAAGGCC
  AGCCGGGGCCTGCGGGCGCTGGTGTTTCTGACCACCAGCCCCCAGGGGCATTTCGTGGGGTCGGACTGGC
  AGCAGAATAGTGTGGTAATCTGTGATGGGCTGGGCCAGGTGGTTGGGGAGTACAAGGGGCCAGGCCTGCA
  TGGCTGCCAGCCGGGCTCCGTGTCTGTGGATAAGAAGGGCTACATCTTTCTGACCCTTCGAGAAGTCAAC
  AAGGTGGTGATCCTGGACCCGAAGGGGTCCCTCCTTGGAGACTTCCTGACAGCCTACCACGGCCTGGAAA
  AGCCCCGGGTTACCACCATGGTGGATGGCAGGTACCTGGTCGTGTCCCTCAGTAACGGGACCATCCACAT
  CTTTCGGGTCCGTTCTCCGGACAGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200891 representing NM_030961
  Red=Cloning site Green=Tags(s)
  
  MVSHGSSPSLLEALSSDFLACKICLEQLRAPKTLPCLHTYCQDCLAQLADGGRVRCPECRETVPVPPEGV
  ASFKTNFFVNGLLDLVKARACGDLRAGKPACALCPLVGGTSTGGPATARCLDCADDLCQACADGHRCTRQ
  THTHRVVDLVGYRAGWYDEEARERQAAQCPQHPGEALRFLCQPCSQLLCRECRLDPHLDHPCLPLAEAVR
  ARRPGLEGLLAGVDNNLVELEAARRVEKEALARLREQAARVGTQVEEAAEGVLRALLAQKQEVLGQLRAH
  VEAAEEAARERLAELEGREQVARAAAAFARRVLSLGREAEILSLEGAIAQRLRQLQGCPWAPGPAPCLLP
  QLELHPGLLDKNCHLLRLSFEEQQPQKDGGKDGAGTQGGEESQSRREDEPKTERQGGVQPQAGDGAQTPK
  EEKAQTTREEGAQTLEEDRAQTPHEDGGPQPHRGGRPNKKKKFKGRLKSISREPSPALGPNLDGSGLLPR
  PIFYCSFPTRMPGDKRSPRITGLCPFGPREILVADEQNRALKRFSLNGDYKGTVPVPEGCSPCSVAALQS
  AVAFSASARLYLINPNGEVQWRRALSLSQASHAVAALPSGDRVAVSVAGHVEVYNMEGSLATRFIPGGKA
  SRGLRALVFLTTSPQGHFVGSDWQQNSVVICDGLGQVVGEYKGPGLHGCQPGSVSVDKKGYIFLTLREVN
  KVVILDPKGSLLGDFLTAYHGLEKPRVTTMVDGRYLVVSLSNGTIHIFRVRSPDS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: AscI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_030961
• ORF Size: 2265 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030961.3
• RefSeq Size: 3547 bp
• RefSeq ORF: 2268 bp
• Locus ID: 81844
• UniProt ID: Q9BRZ2
• Cytogenetics: 7q22.1
• MW: 81.3 kDa
• Gene Summary: E3 ubiquitin-protein ligase that plays a key role in innate antiviral immunity (PubMed:21289118). In response to pathogen- and host-derived double-stranded DNA (dsDNA), targets TMEM173/STING to 'Lys-63'-linked ubiquitination, thereby promoting its homodimerization, a step required for the production of type I interferon IFN-beta (By similarity). Independently of its E3 ubiquitin ligase activity, positive regulator of TLR3 signaling. Potentiates extracellular double stranded RNA (dsRNA)-induced expression of IFNB1 and interferon-stimulated genes ISG15, IFIT1/ISG56, CXCL10, OASL and CCL5/RANTES. Promotes establishment of an antiviral state by TLR3 ligand and TLR3-mediated chemokine induction following infection by hepatitis C virus (PubMed:22948160).[UniProtKB/Swiss-Prot Function]