TRIM56 (NM_030961) Human Tagged ORF Clone

CAT#: RC200891

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TRIM56 (Myc-DDK-tagged)-Human tripartite motif containing 56 (TRIM56)



  "NM_030961" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 477.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol TRIM56
Synonyms RNF109
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC200891 representing NM_030961
Red=Cloning site Blue=ORF Green=Tags(s)

CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC
GCC
C

ATGGTTTCCCACGGGTCCTCGCCCTCCCTCCTGGAGGCCCTGAGCAGCGACTTCCTGGCCTGTAAAATCT
GCCTGGAGCAGCTGCGGGCACCCAAGACACTGCCCTGCCTGCATACCTACTGCCAAGACTGCCTGGCACA
GCTGGCGGATGGCGGCCGCGTCCGCTGCCCCGAGTGCCGCGAGACAGTGCCTGTGCCGCCCGAGGGTGTG
GCCTCCTTCAAGACCAACTTCTTCGTCAATGGGCTGCTGGACCTGGTGAAGGCCCGGGCCTGTGGAGACC
TGCGTGCCGGGAAGCCAGCCTGTGCCCTGTGTCCCCTGGTGGGTGGCACCAGCACCGGGGGGCCGGCCAC
GGCCCGGTGCCTGGACTGTGCCGATGACTTGTGCCAGGCCTGTGCCGACGGGCACCGCTGCACCCGCCAG
ACCCACACCCACCGCGTGGTGGACCTGGTGGGCTACAGGGCCGGGTGGTATGATGAGGAGGCCCGGGAGC
GCCAAGCGGCCCAGTGTCCCCAGCACCCCGGGGAGGCACTGCGCTTCCTGTGCCAGCCCTGCTCACAGTT
GCTGTGCAGAGAGTGCCGCCTAGACCCCCACCTGGACCACCCCTGCCTGCCTCTGGCTGAAGCTGTGCGT
GCCCGGAGGCCGGGCCTGGAGGGACTGCTGGCCGGTGTGGACAATAACCTGGTGGAGCTGGAGGCAGCGC
GGAGGGTGGAGAAGGAGGCGCTAGCCCGGCTGCGGGAGCAGGCGGCCCGGGTGGGGACTCAGGTGGAGGA
GGCGGCTGAGGGCGTCCTCCGGGCCCTGCTGGCCCAGAAGCAGGAGGTGCTGGGGCAGCTACGAGCCCAC
GTGGAGGCTGCCGAAGAAGCTGCTCGGGAGAGGCTGGCGGAGCTTGAGGGCCGGGAGCAGGTGGCCAGGG
CGGCAGCCGCCTTCGCCCGCCGGGTACTCAGCCTGGGGCGAGAGGCCGAGATCCTCTCCCTGGAAGGGGC
GATCGCACAGCGGCTCAGGCAGCTGCAGGGCTGCCCCTGGGCACCAGGCCCGGCCCCCTGCCTGCTCCCA
CAGCTGGAGCTCCATCCTGGGCTCCTGGACAAGAACTGCCACCTTCTTCGGCTGTCCTTTGAGGAGCAGC
AGCCCCAGAAGGATGGTGGGAAAGACGGAGCTGGTACCCAGGGAGGTGAGGAGAGCCAGAGCCGGAGGGA
GGATGAGCCGAAGACTGAGAGACAGGGTGGAGTCCAGCCCCAGGCCGGAGATGGAGCCCAGACCCCAAAA
GAGGAAAAAGCCCAGACAACCCGAGAAGAGGGAGCCCAGACCTTGGAGGAGGACAGGGCCCAGACACCCC
ACGAGGATGGAGGACCCCAGCCCCACAGGGGTGGCAGACCCAACAAGAAGAAAAAGTTCAAAGGCAGGCT
CAAGTCAATTTCCCGGGAGCCCAGCCCAGCCCTGGGGCCGAATCTGGACGGCTCTGGCCTCCTCCCCAGA
CCCATCTTTTACTGCAGTTTCCCCACGCGGATGCCTGGAGACAAGCGGTCCCCCCGGATCACCGGGCTCT
GTCCCTTCGGTCCCCGGGAGATCCTGGTGGCGGATGAGCAGAACCGGGCACTGAAACGCTTCTCCCTCAA
CGGCGACTACAAGGGCACCGTGCCGGTCCCTGAGGGCTGCTCCCCTTGCAGCGTGGCCGCCCTGCAGAGC
GCGGTGGCCTTCTCCGCTAGCGCACGGCTCTATCTCATCAACCCCAATGGCGAAGTGCAGTGGCGCAGGG
CCCTGAGCCTCTCCCAGGCCAGCCACGCGGTGGCGGCACTGCCTAGCGGGGACCGCGTGGCTGTCAGCGT
GGCGGGCCACGTGGAGGTGTACAATATGGAAGGCAGCCTGGCCACCCGGTTCATTCCTGGAGGCAAGGCC
AGCCGGGGCCTGCGGGCGCTGGTGTTTCTGACCACCAGCCCCCAGGGGCATTTCGTGGGGTCGGACTGGC
AGCAGAATAGTGTGGTAATCTGTGATGGGCTGGGCCAGGTGGTTGGGGAGTACAAGGGGCCAGGCCTGCA
TGGCTGCCAGCCGGGCTCCGTGTCTGTGGATAAGAAGGGCTACATCTTTCTGACCCTTCGAGAAGTCAAC
AAGGTGGTGATCCTGGACCCGAAGGGGTCCCTCCTTGGAGACTTCCTGACAGCCTACCACGGCCTGGAAA
AGCCCCGGGTTACCACCATGGTGGATGGCAGGTACCTGGTCGTGTCCCTCAGTAACGGGACCATCCACAT
CTTTCGGGTCCGTTCTCCGGACAGT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC200891 representing NM_030961
Red=Cloning site Green=Tags(s)

MVSHGSSPSLLEALSSDFLACKICLEQLRAPKTLPCLHTYCQDCLAQLADGGRVRCPECRETVPVPPEGV
ASFKTNFFVNGLLDLVKARACGDLRAGKPACALCPLVGGTSTGGPATARCLDCADDLCQACADGHRCTRQ
THTHRVVDLVGYRAGWYDEEARERQAAQCPQHPGEALRFLCQPCSQLLCRECRLDPHLDHPCLPLAEAVR
ARRPGLEGLLAGVDNNLVELEAARRVEKEALARLREQAARVGTQVEEAAEGVLRALLAQKQEVLGQLRAH
VEAAEEAARERLAELEGREQVARAAAAFARRVLSLGREAEILSLEGAIAQRLRQLQGCPWAPGPAPCLLP
QLELHPGLLDKNCHLLRLSFEEQQPQKDGGKDGAGTQGGEESQSRREDEPKTERQGGVQPQAGDGAQTPK
EEKAQTTREEGAQTLEEDRAQTPHEDGGPQPHRGGRPNKKKKFKGRLKSISREPSPALGPNLDGSGLLPR
PIFYCSFPTRMPGDKRSPRITGLCPFGPREILVADEQNRALKRFSLNGDYKGTVPVPEGCSPCSVAALQS
AVAFSASARLYLINPNGEVQWRRALSLSQASHAVAALPSGDRVAVSVAGHVEVYNMEGSLATRFIPGGKA
SRGLRALVFLTTSPQGHFVGSDWQQNSVVICDGLGQVVGEYKGPGLHGCQPGSVSVDKKGYIFLTLREVN
KVVILDPKGSLLGDFLTAYHGLEKPRVTTMVDGRYLVVSLSNGTIHIFRVRSPDS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites AscI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_030961
ORF Size 2265 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_030961.3
RefSeq Size 3547 bp
RefSeq ORF 2268 bp
Locus ID 81844
UniProt ID Q9BRZ2
Cytogenetics 7q22.1
MW 81.3 kDa
Gene Summary E3 ubiquitin-protein ligase that plays a key role in innate antiviral immunity (PubMed:21289118). In response to pathogen- and host-derived double-stranded DNA (dsDNA), targets TMEM173/STING to 'Lys-63'-linked ubiquitination, thereby promoting its homodimerization, a step required for the production of type I interferon IFN-beta (By similarity). Independently of its E3 ubiquitin ligase activity, positive regulator of TLR3 signaling. Potentiates extracellular double stranded RNA (dsRNA)-induced expression of IFNB1 and interferon-stimulated genes ISG15, IFIT1/ISG56, CXCL10, OASL and CCL5/RANTES. Promotes establishment of an antiviral state by TLR3 ligand and TLR3-mediated chemokine induction following infection by hepatitis C virus (PubMed:22948160).[UniProtKB/Swiss-Prot Function]

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