SLC25A19 (NM_021734) Human Tagged ORF Clone

CAT#: RC200868

SLC25A19 (Myc-DDK-tagged)-Human solute carrier family 25 (mitochondrial thiamine pyrophosphate carrier), member 19 (SLC25A19), nuclear gene encoding mitochondrial protein, transcript variant

Product Images

Circular map for RC200868

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  Western blot validation of overexpression lysate (Cat# LY411928) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200868 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SLC25A19
• Synonyms: DNC; MCPHA; MUP1; THMD3; THMD4; TPC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200868 representing NM_021734
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTGGCTATGACCCCAAACCAGATGGCAGGAATAACACCAAGTTCCAGGTGGCAGTGGCTGGGTCTG
  TGTCTGGACTTGTTACTCGGGCGCTGATCAGTCCCTTCGACGTCATCAAGATCCGTTTCCAGCTTCAGCA
  TGAGCGCCTGTCTCGCAGTGACCCCAGCGCAAAGTACCATGGCATCCTCCAGGCCTCTAGGCAGATTCTG
  CAGGAGGAGGGTCCGACAGCTTTCTGGAAAGGACACGTCCCAGCTCAGATTCTCTCCATAGGCTATGGAG
  CTGTCCAATTCTTGTCATTTGAAATGCTGACGGAGCTGGTCCACAGAGGCAGCGTGTATGACGCCCGGGA
  ATTCTCAGTGCACTTTGTATGTGGTGGCCTGGCTGCCTGTATGGCCACCCTCACTGTGCACCCCGTGGAT
  GTTCTGCGCACCCGCTTTGCAGCTCAGGGTGAGCCCAAGGTCTATAATACGCTGCGCCACGCCGTGGGGA
  CCATGTATAGGAGCGAAGGCCCCCAGGTTTTCTACAAAGGCTTGGCTCCCACCTTGATCGCCATCTTCCC
  CTACGCCGGGCTGCAGTTCTCTTGCTACAGCTCCTTGAAGCACCTGTACAAGTGGGCCATACCAGCCGAA
  GGAAAGAAAAATGAGAACCTCCAAAACCTGCTTTGTGGCAGTGGAGCTGGTGTCATCAGCAAGACCCTGA
  CATATCCGCTGGACCTCTTCAAGAAGCGGCTACAGGTTGGAGGGTTTGAGCATGCCAGAGCTGCCTTTGG
  CCAGGTACGGAGATACAAGGGCCTCATGGACTGTGCCAAGCAGGTGCTGCAAAAGGAAGGCGCCCTGGGC
  TTCTTCAAGGGCCTGTCCCCCAGCTTGCTGAAGGCTGCCCTCTCCACAGGCTTCATGTTCTTCTCGTATG
  AATTCTTCTGTAATGTCTTCCACTGCATGAACAGGACAGCCAGCCAGCGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200868 representing NM_021734
  Red=Cloning site Green=Tags(s)
  
  MVGYDPKPDGRNNTKFQVAVAGSVSGLVTRALISPFDVIKIRFQLQHERLSRSDPSAKYHGILQASRQIL
  QEEGPTAFWKGHVPAQILSIGYGAVQFLSFEMLTELVHRGSVYDAREFSVHFVCGGLAACMATLTVHPVD
  VLRTRFAAQGEPKVYNTLRHAVGTMYRSEGPQVFYKGLAPTLIAIFPYAGLQFSCYSSLKHLYKWAIPAE
  GKKNENLQNLLCGSGAGVISKTLTYPLDLFKKRLQVGGFEHARAAFGQVRRYKGLMDCAKQVLQKEGALG
  FFKGLSPSLLKAALSTGFMFFSYEFFCNVFHCMNRTASQR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021734
• ORF Size: 960 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021734.4
• RefSeq Size: 1651 bp
• RefSeq ORF: 963 bp
• Locus ID: 60386
• UniProt ID: Q9HC21
• Cytogenetics: 17q25.1
• Domains: mito_carr
• Protein Families: Druggable Genome
• MW: 35.3 kDa
• Gene Summary: This gene encodes a mitochondrial protein that is a member of the solute carrier family. Although this protein was initially thought to be the mitochondrial deoxynucleotide carrier involved in the uptake of deoxynucleotides into the matrix of the mitochondria, further studies have demonstrated that this protein instead functions as the mitochondrial thiamine pyrophosphate carrier, which transports thiamine pyrophosphates into mitochondria. Mutations in this gene cause microcephaly, Amish type, a metabolic disease that results in severe congenital microcephaly, severe 2-ketoglutaric aciduria, and death within the first year. Multiple alternatively spliced variants, encoding the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]