C11orf73 (HIKESHI) (NM_016401) Human Tagged ORF Clone

CAT#: RC200866

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C11orf73 (Myc-DDK-tagged)-Human chromosome 11 open reading frame 73 (C11orf73), transcript variant 1



  "NM_016401" in other vectors (4)

Reconstitution Protocol

USD 300.00

USD 450.00

In Stock*

Size
    • 10 ug

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Frequently bought together (4)
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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol HIKESHI
Synonyms C11orf73; HLD13; HSPC138; HSPC179; L7RN6; OPI10
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC200866 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTTGGCTGCTTGGTGGCGGGGAGGCTGGTGCAAACAGCTGCACAGCAAGTGGCAGAGGATAAATTTG
TTTTTGACTTACCTGATTATGAAAGTATCAACCATGTTGTGGTTTTTATGCTGGGAACAATCCCATTTCC
TGAGGGAATGGGAGGATCTGTCTACTTTTCTTATCCTGATTCAAATGGAATGCCAGTATGGCAACTCCTA
GGATTTGTCACGAATGGGAAGCCAAGTGCCATCTTCAAAATTTCAGGTCTTAAATCTGGAGAAGGAAGCC
AACATCCTTTTGGAGCCATGAATATTGTCCGAACTCCATCTGTTGCTCAGATTGGAATTTCAGTGGAATT
ATTAGACAGTATGGCTCAGCAGACTCCTGTAGGTAATGCTGCTGTATCCTCAGTTGACTCATTCACTCAG
TTCACACAAAAGATGTTGGACAATTTCTACAATTTTGCTTCATCATTTGCTGTCTCTCAGGCCCAGATGA
CACCAAGCCCATCTGAAATGTTCATTCCGGCAAATGTGGTTCTGAAATGGTATGAAAACTTTCAAAGACG
ACTAGCACAGAACCCTCTCTTTTGGAAAACA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC200866 protein sequence
Red=Cloning site Green=Tags(s)

MFGCLVAGRLVQTAAQQVAEDKFVFDLPDYESINHVVVFMLGTIPFPEGMGGSVYFSYPDSNGMPVWQLL
GFVTNGKPSAIFKISGLKSGEGSQHPFGAMNIVRTPSVAQIGISVELLDSMAQQTPVGNAAVSSVDSFTQ
FTQKMLDNFYNFASSFAVSQAQMTPSPSEMFIPANVVLKWYENFQRRLAQNPLFWKT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_016401
ORF Size 591 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_016401.4
RefSeq Size 1187 bp
RefSeq ORF 594 bp
Locus ID 51501
UniProt ID Q53FT3
Cytogenetics 11q14.2
MW 21.6 kDa
Gene Summary This gene encodes an evolutionarily conserved nuclear transport receptor that mediates heat-shock-induced nuclear import of 70 kDa heat-shock proteins (Hsp70s) through interactions with FG-nucleoporins. The protein mediates transport of the ATP form but not the ADP form of Hsp70 proteins under conditions of heat shock stress. Structural analyses demonstrate that the protein forms an asymmetric homodimer and that the N-terminal domain consists of a jelly-roll/beta-sandwich fold structure that contains hydrophobic pockets involved in FG-nucleoporin recognition. Reduction of RNA expression levels in HeLa cells using small interfering RNAs results in inhibition of heat shock-induced nuclear accumulation of Hsp70s, indicating a role for this gene in regulation of Hsp70 nuclear import during heat shock stress. [provided by RefSeq, Apr 2016]

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