GTPBP3 (NM_032620) Human Tagged ORF Clone

CAT#: RC200836

GTPBP3 (Myc-DDK-tagged)-Human GTP binding protein 3 (mitochondrial) (GTPBP3), nuclear gene encoding mitochondrial protein, transcript variant V

Product Images

Circular map for RC200836

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  Western blot validation of overexpression lysate (Cat# LY410007) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200836 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified GTPBP3 protein (Cat# TP300836). The protein was produced from HEK293T cells transfected with GTPBP3 cDNA clone (Cat# RC200836) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GTPBP3
• Synonyms: COXPD23; GTPBG3; MSS1; MTGP1; THDF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200836 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGCGGGGGCTTTGGACCCTGGCGGCCCAAGCGGCACGTGGGCCTCGCAGATTGTGCACGCGCCGGA
  GCAGCGGCGCACCAGCCCCCGGCTCCGGCGCCACCATCTTCGCGCTAAGCTCTGGCCAAGGCCGCTGCGG
  CATCGCAGTGATCCGGACCAGCGGCCCCGCCAGCGGCCACGCCCTCCGAATTCTCACAGCACCCCGAGAC
  CTGCCCCTTGCTCGCCACGCCAGCCTGCGCCTGCTCAGCGATCCCCGCTCCGGGGAGCCTCTGGACCGCG
  CACTGGTGCTCTGGTTCCCAGGTCCCCAGAGTTTCACCGGTGAGGACTGCGTGGAGTTCCACGTGCATGG
  AGGCCCGGCAGTGGTGAGCGGCGTCCTGCAGGCCTTGGGCAGCGTGCCAGGGCTTCGACCGGCGGAGGCA
  GGCGAGTTCACCAGACGGGCGTTCGCCAATGGGAAGCTGAACCTGACCGAAGTGGAGGGGCTGGCGGACC
  TTATCCACGCGGAAACAGAGGCGCAGCGGCGGCAGGCCCTCAGGCAGCTGGACGGAGAGCTGGGCCACCT
  CTGCCGTGGCTGGGCCGAGACCCTCACCAAAGCTCTGGCCCACGTGGAGGCCTATATCGATTTCGGCGAG
  GATGACAACCTGGAGGAGGGGGTCCTGGAGCAAGCCGACATCGAAGTACGGGCACTGCAGGTGGCCCTGG
  GTGCACATCTACGAGATGCCAGGCGCGGGCAGAGGCTCCGCTCAGGGGTGCACGTAGTGGTCACTGGACC
  CCCCAATGCGGGCAAGAGCAGCCTAGTGAACCTGCTCAGTCGGAAGCCTGTGTCCATCGTGTCCCCGGAG
  CCAGGGACCACCCGTGACGTGCTGGAGACCCCAGTCGACCTGGCCGGATTTCCTGTGCTGCTGAGCGACA
  CGGCTGGGTTGCGGGAGGGCGTGGGGCCCGTGGAGCAGGAGGGCGTGCGGCGCGCCCGGGAGAGGCTAGA
  GCAGGCTGACCTCATTCTGGCCATGCTGGATGCTTCTGACCTGGCCTCTCCCTCCAGTTGCAACTTCCTG
  GCCACCGTCGTAGCCTCTGTGGGAGCCCAGAGCCCCAGTGACAGCAGCCAGCACCTCCTCCTGGTGCTGA
  ACAAGTCGGACCTGCTGTCCCCGGAGGGCCCAGGTCCCGGTCCTGACCTGCCCCCGCACCTGCTGCTGTC
  CTGTCTGACGGGAGAGGGGCTGGACGGCCTCCTGGAGGCGCTGAGGAAGGAGCTAGCTGCAGTGTGTGGG
  GACCCGTCCACAGATCCCCCGCTGCTGACCCGAGCAAGGCACCAGCACCACCTCCAGGGTTGCCTGGATG
  CCCTCGGCCACTACAAGCAGTCAAAAGACCTGGCCCTGGCGGCAGAGGCGCTGCGGGTGGCCCGGGGTCA
  CCTGACCCGGCTCACAGGTGGAGGGGGTACCGAGGAGATCCTGGACATCATCTTCCAGGACTTCTGTGTG
  GGCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200836 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MWRGLWTLAAQAARGPRRLCTRRSSGAPAPGSGATIFALSSGQGRCGIAVIRTSGPASGHALRILTAPRD
  LPLARHASLRLLSDPRSGEPLDRALVLWFPGPQSFTGEDCVEFHVHGGPAVVSGVLQALGSVPGLRPAEA
  GEFTRRAFANGKLNLTEVEGLADLIHAETEAQRRQALRQLDGELGHLCRGWAETLTKALAHVEAYIDFGE
  DDNLEEGVLEQADIEVRALQVALGAHLRDARRGQRLRSGVHVVVTGPPNAGKSSLVNLLSRKPVSIVSPE
  PGTTRDVLETPVDLAGFPVLLSDTAGLREGVGPVEQEGVRRARERLEQADLILAMLDASDLASPSSCNFL
  ATVVASVGAQSPSDSSQHLLLVLNKSDLLSPEGPGPGPDLPPHLLLSCLTGEGLDGLLEALRKELAAVCG
  DPSTDPPLLTRARHQHHLQGCLDALGHYKQSKDLALAAEALRVARGHLTRLTGGGGTEEILDIIFQDFCV
  GK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032620
• ORF Size: 1476 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032620.4
• RefSeq Size: 2612 bp
• RefSeq ORF: 1479 bp
• Locus ID: 84705
• UniProt ID: Q969Y2
• Cytogenetics: 19p13.11
• MW: 52 kDa
• Gene Summary: This locus encodes a GTP-binding protein. The encoded protein is localized to the mitochondria and may play a role in mitochondrial tRNA modification. Polymorphisms at this locus may be associated with severity of aminoglycoside-induced deafness, a disease associated with a mutation in the 12S rRNA. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Sep 2010]