RAMAC (NM_031452) Human Tagged ORF Clone

CAT#: RC200815

FAM103A1 (Myc-DDK-tagged)-Human family with sequence similarity 103, member A1 (FAM103A1)

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK w/ Puro mGFP w/ Puro

AAV Particle: DDK


  "NM_031452" in other vectors (4)

Reconstitution Protocol

USD 150.00

In Stock*

Size
    • 10 ug

Product Images

Frequently bought together (4)
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Other products for "RAMAC"

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol RAMAC
Synonyms C15orf18; FAM103A1; HsT19360; RAM; RAMMET
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC200815 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACTGACACTGCCGAAGCTGTTCCAAAGTTTGAAGAGATGTTTGCTAGTAGATTCACAGAAAATGACA
AGGAGTATCAGGAATACCTGAAACGCCCTCCTGAGTCTCCTCCAATTGTTGAGGAATGGAATAGCAGAGC
TGGTGGGAACCAAAGAAACAGAGGCAATCGGTTGCAAGACAACAGACAGTTCAGAGGCAGGGACAACAGA
TGGGGGTGGCCAAGTGACAATCGATCCAATCAGTGGCATGGACGATCCTGGGGTAACAACTACCCGCAAC
ACAGACAAGAACCTTACTATCCCCAGCAATATGGACATTATGGTTACAACCAGCGGCCTCCTTACGGTTA
CTAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC200815 protein sequence
Red=Cloning site Green=Tags(s)

MTDTAEAVPKFEEMFASRFTENDKEYQEYLKRPPESPPIVEEWNSRAGGNQRNRGNRLQDNRQFRGRDNR
WGWPSDNRSNQWHGRSWGNNYPQHRQEPYYPQQYGHYGYNQRPPYGYY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_031452
ORF Size 354 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_031452.4
RefSeq Size 1588 bp
RefSeq ORF 357 bp
Locus ID 83640
UniProt ID Q9BTL3
Cytogenetics 15q25.2
MW 14.4 kDa
Gene Summary Regulatory subunit of the mRNA-capping methyltransferase RNMT:RAMAC complex that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs (PubMed:22099306, PubMed:27422871). Promotes the recruitment of the methyl donor, S-adenosyl-L-methionine, to RNMT (PubMed:27422871). Regulates RNMT expression by a post-transcriptional stabilizing mechanism (PubMed:22099306). Binds RNA (PubMed:22099306).[UniProtKB/Swiss-Prot Function]

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.