PRP19 (PRPF19) (NM_014502) Human Tagged ORF Clone

CAT#: RC200810

PRPF19 (Myc-DDK-tagged)-Human PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae) (PRPF19)

Product Images

Western blot validation of overexpression lysate (Cat# LY402343) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200810 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified PRPF19 protein (Cat# TP300810). The protein was produced from HEK293T cells transfected with PRPF19 cDNA clone (Cat# RC200810) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PRPF19
• Synonyms: hPSO4; NMP200; PRP19; PSO4; SNEV; UBOX4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200810 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCTAATCTGCTCCATCTCTAACGAAGTGCCGGAGCACCCATGTGTATCCCCTGTCTCTAATCATG
  TTTATGAGCGGCGGCTCATCGAGAAGTACATTGCGGAGAATGGTACCGACCCCATCAACAACCAGCCTCT
  CTCCGAGGAGCAGCTCATCGACATCAAAGTTGCTCACCCAATCCGGCCCAAGCCTCCCTCAGCCACCAGC
  ATCCCGGCCATTCTGAAAGCTTTGCAGGATGAGTGGGATGCAGTCATGCTGCACAGCTTCACTCTGCGCC
  AGCAGCTGCAGACAACCCGCCAAGAGCTGTCACACGCTCTGTACCAGCACGATGCCGCCTGCCGTGTCAT
  TGCCCGTCTCACCAAGGAAGTCACTGCTGCCCGAGAAGCTCTGGCTACCCTGAAACCACAGGCTGGCCTC
  ATTGTGCCCCAGGCTGTGCCAAGTTCCCAACCAAGTGTTGTGGGTGCGGGTGAGCCAATGGATTTGGGTG
  AGCTGGTGGGAATGACCCCAGAGATTATTCAGAAGCTTCAAGACAAAGCCACTGTGCTAACCACGGAGCG
  CAAGAAGAGAGGGAAGACTGTGCCTGAGGAGCTGGTGAAGCCAGAAGAGCTCAGCAAATACCGGCAGGTG
  GCATCCCACGTGGGGTTGCACAGTGCCAGCATTCCTGGGATCCTGGCCCTGGACCTCTGCCCGTCCGACA
  CCAACAAGATCCTCACTGGTGGGGCGGATAAAAATGTCGTTGTGTTTGACAAAAGTTCTGAACAAATCCT
  GGCTACCCTCAAAGGCCATACCAAGAAGGTCACCAGCGTGGTGTTTCACCCTTCCCAGGACCTGGTGTTT
  TCTGCTTCCCCCGATGCCACTATCAGGATTTGGTCGGTCCCCAATGCCTCTTGTGTACAGGTGGTTCGGG
  CCCATGAGAGTGCTGTGACAGGCCTCAGCCTTCATGCCACTGGCGACTATCTCCTGAGCTCCTCCGATGA
  TCAGTACTGGGCTTTCTCTGACATCCAGACAGGGCGTGTGCTCACCAAGGTGACAGATGAGACCTCCGGC
  TGCTCTCTCACCTGTGCACAGTTCCACCCTGACGGACTCATCTTTGGAACAGGAACCATGGACTCTCAGA
  TCAAGATCTGGGACTTGAAGGAACGTACTAATGTGGCCAACTTCCCTGGCCACTCGGGCCCCATCACTAG
  CATCGCCTTCTCTGAGAATGGTTACTACCTGGCTACAGCGGCTGATGACTCCTCTGTCAAGCTCTGGGAT
  CTGCGCAAGCTTAAGAACTTTAAGACTTTGCAGCTGGATAACAACTTTGAGGTAAAGTCACTGATCTTTG
  ACCAGAGTGGTACCTACCTGGCTCTTGGGGGCACGGATGTCCAGATCTACATCTGCAAACAATGGACGGA
  GATTCTTCACTTTACAGAGCATAGCGGCCTGACCACAGGGGTGGCCTTCGGGCATCACGCCAAGTTCATC
  GCTTCAACAGGCATGGACAGAAGCCTCAAGTTCTACAGCCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200810 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSLICSISNEVPEHPCVSPVSNHVYERRLIEKYIAENGTDPINNQPLSEEQLIDIKVAHPIRPKPPSATS
  IPAILKALQDEWDAVMLHSFTLRQQLQTTRQELSHALYQHDAACRVIARLTKEVTAAREALATLKPQAGL
  IVPQAVPSSQPSVVGAGEPMDLGELVGMTPEIIQKLQDKATVLTTERKKRGKTVPEELVKPEELSKYRQV
  ASHVGLHSASIPGILALDLCPSDTNKILTGGADKNVVVFDKSSEQILATLKGHTKKVTSVVFHPSQDLVF
  SASPDATIRIWSVPNASCVQVVRAHESAVTGLSLHATGDYLLSSSDDQYWAFSDIQTGRVLTKVTDETSG
  CSLTCAQFHPDGLIFGTGTMDSQIKIWDLKERTNVANFPGHSGPITSIAFSENGYYLATAADDSSVKLWD
  LRKLKNFKTLQLDNNFEVKSLIFDQSGTYLALGGTDVQIYICKQWTEILHFTEHSGLTTGVAFGHHAKFI
  ASTGMDRSLKFYSL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014502
• ORF Size: 1512 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014502.5
• RefSeq Size: 2342 bp
• RefSeq ORF: 1515 bp
• Locus ID: 27339
• UniProt ID: Q9UMS4
• Cytogenetics: 11q12.2
• Domains: WD40, U-box
• Protein Families: Druggable Genome
• Protein Pathways: Spliceosome, Ubiquitin mediated proteolysis
• MW: 55.2 kDa
• Gene Summary: PSO4 is the human homolog of yeast Pso4, a gene essential for cell survival and DNA repair (Beck et al., 2008 [PubMed 18263876]).[supplied by OMIM, Sep 2008]