L3MBTL2 (NM_031488) Human Tagged ORF Clone

CAT#: RC200781

L3MBTL2 (Myc-DDK-tagged)-Human l(3)mbt-like 2 (Drosophila) (L3MBTL2)

Product Images

Circular map for RC200781

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  Western blot validation of overexpression lysate (Cat# LY410485) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200781 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified L3MBTL2 protein (Cat# TP300781). The protein was produced from HEK293T cells transfected with L3MBTL2 cDNA clone (Cat# RC200781) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L3MBTL2
• Synonyms: H-l(3)mbt-l; L3MBT
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200781 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAAGCCCCGGAGTATTGAGGAGACCCCATCTTCAGAACCAATGGAGGAAGAGGAAGATGACGACT
  TGGAGCTGTTTGGTGGCTATGATAGTTTCCGGAGTTATAACAGCAGTGTGGGCAGTGAGAGCAGCTCCTA
  TCTGGAGGAGTCAAGTGAAGCAGAAAATGAGGATCGGGAAGCAGGGGAACTGCCGACCTCCCCGCTGCAT
  TTGCTCAGCCCTGGGACTCCTCGCTCCTTGGATGGCAGTGGTTCTGAGCCAGCTGTCTGTGAGATGTGTG
  GTATCGTGGGTACAAGGGAAGCCTTCTTCTCCAAGACCAAGAGGTTCTGCAGCGTCTCCTGCTCCAGGAG
  CTACTCCTCCAACTCCAAGAAAGCCAGTATCTTGGCTAGATTACAGGGAAAACCACCGACCAAAAAAGCC
  AAAGTCCTGCACAAGGCTGCCTGGTCTGCCAAAATTGGAGCCTTCCTCCACTCTCAAGGGACAGGACAGC
  TGGCAGATGGGACACCAACAGGACAAGACGCTCTGGTCTTGGGCTTCGACTGGGGGAAGTTCCTGAAGGA
  TCACAGTTACAAGGCTGCTCCCGTCAGCTGTTTCAAGCACGTCCCACTCTATGACCAGTGGGAGGATGTG
  ATGAAAGGGATGAAGGTGGAGGTGCTCAACAGTGATGCTGTGCTCCCCAGCCGGGTGTACTGGATCGCCT
  CTGTCATCCAGACAGCAGGGTATCGGGTGCTGCTTCGGTATGAAGGCTTTGAAAATGACGCCAGCCATGA
  CTTCTGGTGCAACCTGGGAACAGTGGATGTCCACCCCATTGGCTGGTGTGCCATCAACAGCAAGATCCTA
  GTGCCCCCACGGACCATCCATGCCAAGTTCACCGACTGGAAGGGCTACCTCATGAAACGGCTGGTGGGCT
  CCAGGACGCTTCCCGTGGATTTCCACATCAAGATGGTGGAGAGCATGAAGTACCCCTTTAGGCAGGGCAT
  GCGGCTGGAAGTGGTGGACAAGTCCCAGGTGTCACGCACTCGCATGGCTGTGGTGGACACAGTAATCGGG
  GGTCGCCTACGGCTCCTCTACGAGGATGGTGACAGTGACGACGACTTCTGGTGCCACATGTGGAGCCCCC
  TGATCCACCCAGTGGGTTGGTCACGACGTGTGGGCCACGGCATCAAGATGTCAGAGAGGCGAAGTGACAT
  GGCCCATCACCCCACCTTCCGGAAGATCTACTGTGATGCCGTTCCTTACCTCTTCAAGAAGGTACGAGCA
  GTCTACACAGAAGGCGGTTGGTTTGAGGAAGGGATGAAGCTGGAGGCCATTGACCCCCTGAATCTGGGCA
  ACATCTGCGTGGCAACTGTCTGTAAGGTTCTCCTGGATGGATACCTGATGATCTGTGTGGACGGGGGGCC
  CTCCACAGATGGCTTGGACTGGTTCTGCTACCATGCCTCTTCCCACGCCATCTTCCCGGCCACCTTCTGT
  CAGAAGAATGACATTGAGCTCACACCGCCAAAAGGTTATGAGGCACAGACTTTCAACTGGGAGAACTACT
  TGGAGAAGACCAAGTCGAAAGCCGCTCCATCGAGACTCTTTAACATGGATTGCCCAAACCATGGCTTCAA
  GGTGGGCATGAAGCTGGAGGCCGTGGACCTGATGGAGCCCCGGCTCATCTGTGTGGCCACGGTGAAACGA
  GTGGTGCATCGGCTCCTCAGCATCCACTTTGACGGCTGGGACAGCGAGTACGACCAGTGGGTGGACTGCG
  AGTCCCCAGACATCTACCCCGTCGGCTGGTGTGAGCTCACCGGCTACCAGCTCCAGCCTCCTGTGGCCGC
  AGAACCGGCCACACCGCTGAAGGCCAAAGAGGCCACAAAGAAGAAAAAGAAACAGTTTGGGAAGAAAAGG
  AAAAGAATCCCGCCCACTAAGACGCGACCCCTCAGACAGGGGTCCAAGAAGCCCCTGCTGGAGGACGACC
  CTCAGGGTGCCAGGAAGATCTCGTCGGAGCCTGTTCCTGGCGAGATCATTGCTGTGCGTGTGAAGGAAGA
  GCATCTAGACGTGGCCTCGCCCGACAAGGCTTCAAGTCCAGAGCTGCCTGTCTCCGTCGAGAACATCAAG
  CAGGAAACAGACGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200781 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEKPRSIEETPSSEPMEEEEDDDLELFGGYDSFRSYNSSVGSESSSYLEESSEAENEDREAGELPTSPLH
  LLSPGTPRSLDGSGSEPAVCEMCGIVGTREAFFSKTKRFCSVSCSRSYSSNSKKASILARLQGKPPTKKA
  KVLHKAAWSAKIGAFLHSQGTGQLADGTPTGQDALVLGFDWGKFLKDHSYKAAPVSCFKHVPLYDQWEDV
  MKGMKVEVLNSDAVLPSRVYWIASVIQTAGYRVLLRYEGFENDASHDFWCNLGTVDVHPIGWCAINSKIL
  VPPRTIHAKFTDWKGYLMKRLVGSRTLPVDFHIKMVESMKYPFRQGMRLEVVDKSQVSRTRMAVVDTVIG
  GRLRLLYEDGDSDDDFWCHMWSPLIHPVGWSRRVGHGIKMSERRSDMAHHPTFRKIYCDAVPYLFKKVRA
  VYTEGGWFEEGMKLEAIDPLNLGNICVATVCKVLLDGYLMICVDGGPSTDGLDWFCYHASSHAIFPATFC
  QKNDIELTPPKGYEAQTFNWENYLEKTKSKAAPSRLFNMDCPNHGFKVGMKLEAVDLMEPRLICVATVKR
  VVHRLLSIHFDGWDSEYDQWVDCESPDIYPVGWCELTGYQLQPPVAAEPATPLKAKEATKKKKKQFGKKR
  KRIPPTKTRPLRQGSKKPLLEDDPQGARKISSEPVPGEIIAVRVKEEHLDVASPDKASSPELPVSVENIK
  QETDD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031488
• ORF Size: 2115 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031488.3
• RefSeq Size: 3205 bp
• RefSeq ORF: 2118 bp
• Locus ID: 83746
• UniProt ID: Q969R5
• Cytogenetics: 22q13.2
• Domains: MBT
• Protein Families: Transcription Factors
• MW: 79.1 kDa
• Gene Summary: Putative Polycomb group (PcG) protein. PcG proteins maintain the transcriptionally repressive state of genes, probably via a modification of chromatin, rendering it heritably changed in its expressibility. Its association with a chromatin-remodeling complex suggests that it may contribute to prevent expression of genes that trigger the cell into mitosis. Binds to monomethylated and dimethylated 'Lys-20' on histone H4. Binds histone H3 peptides that are monomethylated or dimethylated on 'Lys-4', 'Lys-9' or 'Lys-27'.[UniProtKB/Swiss-Prot Function]