MAN2B1 (NM_000528) Human Tagged ORF Clone

CAT#: RC200638

MAN2B1 (Myc-DDK-tagged)-Human mannosidase, alpha, class 2B, member 1 (MAN2B1), transcript variant 1

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro


  "NM_000528" in other vectors (6)

Reconstitution Protocol

USD 880.00

In Stock*

Size
    • 10 ug

Product Images

Frequently bought together (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

USD 650.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

USD 160.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

USD 59.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

USD 198.00


MAN2B1 Rabbit polyclonal Antibody
    • 100 ul

USD 365.00

Other products for "MAN2B1"

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol MAN2B1
Synonyms LAMAN; MANB
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC200638 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCGCCTACGCGCGGGCTTCGGGGGTCTGCGCTCGCGGCTGCCTGGACTCAGCAGGCCCCTGGACCA
TGTCCCGCGCCCTGCGGCCACCGCTCCCGCCTCTCTGCTTTTTCCTTTTGTTGCTGGCGGCTGCCGGTGC
TCGGGCCGGGGGATACGAGACATGCCCCACAGTGCAGCCGAACATGCTGAACGTGCACCTGCTGCCTCAC
ACACATGATGACGTGGGCTGGCTCAAAACCGTGGACCAGTACTTTTATGGAATCAAGAATGACATCCAGC
ACGCCGGTGTGCAGTACATCCTGGACTCGGTCATCTCTGCCTTGCTGGCAGATCCCACCCGTCGCTTCAT
TTACGTGGAGATTGCCTTCTTCTCCCGTTGGTGGCACCAGCAGACAAATGCCACACAGGAAGTCGTGCGA
GACCTTGTGCGCCAGGGGCGCCTGGAGTTCGCCAATGGTGGCTGGGTGATGAACGATGAGGCAGCCACCC
ACTACGGTGCCATCGTGGACCAGATGACACTTGGGCTGCGCTTTCTGGAGGACACATTTGGCAATGATGG
GCGACCCCGTGTGGCCTGGCACATTGACCCCTTCGGCCACTCTCGGGAGCAGGCCTCGCTGTTTGCGCAG
ATGGGCTTCGACGGCTTCTTCTTTGGGCGCCTTGATTATCAAGATAAGTGGGTACGGATGCAGAAGCTGG
AGATGGAGCAGGTGTGGCGGGCCAGCACCAGCCTGAAGCCCCCGACCGCGGACCTCTTCACTGGTGTGCT
TCCCAATGGTTACAACCCGCCAAGGAATCTGTGCTGGGATGTGCTGTGTGTCGATCAGCCGCTGGTGGAG
GACCCTCGCAGCCCCGAGTACAACGCCAAGGAGCTGGTCGATTACTTCCTAAATGTGGCCACTGCCCAGG
GCCGGTATTACCGCACCAACCACACTGTGATGACCATGGGCTCGGACTTCCAATATGAGAATGCCAACAT
GTGGTTCAAGAACCTTGACAAGCTCATCCGGCTGGTAAATGCGCAGCAGGCAAAAGGAAGCAGTGTCCAT
GTTCTCTACTCCACCCCCGCTTGTTACCTCTGGGAGCTGAACAAGGCCAACCTCACCTGGTCAGTGAAAC
ATGACGACTTCTTCCCTTACGCGGATGGCCCCCACCAGTTCTGGACCGGTTACTTTTCCAGTCGGCCGGC
CCTCAAACGCTACGAGCGCCTCAGCTACAACTTCCTGCAGGTGTGCAACCAGCTGGAGGCGCTGGTGGGC
CTGGCGGCCAACGTGGGACCCTATGGCTCCGGAGACAGTGCACCCCTCAATGAGGCGATGGCTGTGCTCC
AGCATCACGACGCCGTCAGCGGCACCTCCCGCCAGCACGTGGCCAACGACTACGCGCGCCAGCTTGCGGC
AGGCTGGGGGCCTTGCGAGGTTCTTCTGAGCAACGCGCTGGCGCGGCTCAGAGGCTTCAAAGATCACTTC
ACCTTTTGCCAACAGCTAAACATCAGCATCTGCCCGCTCAGCCAGACGGCGGCGCGCTTCCAGGTCATCG
TTTATAATCCCCTGGGGCGGAAGGTGAATTGGATGGTACGGCTGCCGGTCAGCGAAGGCGTTTTCGTTGT
GAAGGACCCCAATGGCAGGACAGTGCCCAGCGATGTGGTAATATTTCCCAGCTCAGACAGCCAGGCGCAC
CCTCCGGAGCTGCTGTTCTCAGCCTCACTGCCCGCCCTGGGCTTCAGCACCTATTCAGTAGCCCAGGTGC
CTCGCTGGAAGCCCCAGGCCCGCGCACCACAGCCCATCCCCAGAAGATCCTGGTCCCCTGCTTTAACCAT
CGAAAATGAGCACATCCGGGCAACGTTTGATCCTGACACAGGGCTGTTGATGGAGATTATGAACATGAAT
CAGCAACTCCTGCTGCCTGTTCGCCAGACCTTCTTCTGGTACAACGCCAGTATAGGTGACAACGAAAGTG
ACCAGGCCTCAGGTGCCTACATCTTCAGACCCAACCAACAGAAACCGCTGCCTGTGAGCCGCTGGGCTCA
GATCCACCTGGTGAAGACACCCTTGGTGCAGGAGGTGCACCAGAACTTCTCAGCTTGGTGTTCCCAGGTG
GTTCGCCTGTACCCAGGACAGCGGCACCTGGAGCTAGAGTGGTCGGTGGGGCCGATACCTGTGGGCGACA
CCTGGGGGAAGGAGGTCATCAGCCGTTTTGACACACCGCTGGAGACAAAGGGACGCTTCTACACAGACAG
CAATGGCCGGGAGATCCTGGAGAGGAGGCGGGATTATCGACCCACCTGGAAACTGAACCAGACGGAGCCC
GTGGCAGGAAACTACTATCCAGTCAACACCCGGATTTACATCACGGATGGAAACATGCAGCTGACTGTGC
TGACTGACCGCTCCCAGGGGGGCAGCAGCCTGAGAGATGGCTCGCTGGAGCTCATGGTGCACCGAAGGCT
GCTGAAGGACGATGGACGCGGAGTATCGGAGCCACTAATGGAGAACGGGTCGGGGGCGTGGGTGCGAGGG
CGCCACCTGGTGCTGCTGGACACAGCCCAGGCTGCAGCCGCCGGACACCGGCTCCTGGCGGAGCAGGAGG
TCCTGGCCCCTCAGGTGGTGCTGGCCCCGGGTGGCGGCGCCGCCTACAATCTCGGGGCTCCTCCGCGCAC
GCAGTTCTCAGGGCTGCGCAGGGACCTGCCGCCCTCGGTGCACCTGCTCACGCTGGCCAGCTGGGGCCCC
GAAATGGTGCTGCTGCGCTTGGAGCACCAGTTTGCCGTAGGAGAGGATTCCGGACGTAACCTGAGCGCCC
CCGTTACCTTGAACTTGAGGGACCTGTTCTCCACCTTCACCATCACCCGCCTGCAGGAGACCACGCTGGT
GGCCAACCAGCTCCGCGAGGCAGCCTCCAGGCTCAAGTGGACAACAAACACAGGCCCCACACCCCACCAA
ACTCCGTACCAGCTGGACCCGGCCAACATCACGCTGGAACCCATGGAAATCCGCACTTTCCTGGCCTCAG
TTCAATGGAAGGAGGTGGATGGT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC200638 protein sequence
Red=Cloning site Green=Tags(s)

MGAYARASGVCARGCLDSAGPWTMSRALRPPLPPLCFFLLLLAAAGARAGGYETCPTVQPNMLNVHLLPH
THDDVGWLKTVDQYFYGIKNDIQHAGVQYILDSVISALLADPTRRFIYVEIAFFSRWWHQQTNATQEVVR
DLVRQGRLEFANGGWVMNDEAATHYGAIVDQMTLGLRFLEDTFGNDGRPRVAWHIDPFGHSREQASLFAQ
MGFDGFFFGRLDYQDKWVRMQKLEMEQVWRASTSLKPPTADLFTGVLPNGYNPPRNLCWDVLCVDQPLVE
DPRSPEYNAKELVDYFLNVATAQGRYYRTNHTVMTMGSDFQYENANMWFKNLDKLIRLVNAQQAKGSSVH
VLYSTPACYLWELNKANLTWSVKHDDFFPYADGPHQFWTGYFSSRPALKRYERLSYNFLQVCNQLEALVG
LAANVGPYGSGDSAPLNEAMAVLQHHDAVSGTSRQHVANDYARQLAAGWGPCEVLLSNALARLRGFKDHF
TFCQQLNISICPLSQTAARFQVIVYNPLGRKVNWMVRLPVSEGVFVVKDPNGRTVPSDVVIFPSSDSQAH
PPELLFSASLPALGFSTYSVAQVPRWKPQARAPQPIPRRSWSPALTIENEHIRATFDPDTGLLMEIMNMN
QQLLLPVRQTFFWYNASIGDNESDQASGAYIFRPNQQKPLPVSRWAQIHLVKTPLVQEVHQNFSAWCSQV
VRLYPGQRHLELEWSVGPIPVGDTWGKEVISRFDTPLETKGRFYTDSNGREILERRRDYRPTWKLNQTEP
VAGNYYPVNTRIYITDGNMQLTVLTDRSQGGSSLRDGSLELMVHRRLLKDDGRGVSEPLMENGSGAWVRG
RHLVLLDTAQAAAAGHRLLAEQEVLAPQVVLAPGGGAAYNLGAPPRTQFSGLRRDLPPSVHLLTLASWGP
EMVLLRLEHQFAVGEDSGRNLSAPVTLNLRDLFSTFTITRLQETTLVANQLREAASRLKWTTNTGPTPHQ
TPYQLDPANITLEPMEIRTFLASVQWKEVDG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_000528
ORF Size 3033 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000528.4
RefSeq Size 3231 bp
RefSeq ORF 3036 bp
Locus ID 4125
UniProt ID O00754
Cytogenetics 19p13.13
Domains Glyco_hydro_38
Protein Families Druggable Genome
Protein Pathways Lysosome, Other glycan degradation
MW 113.7 kDa
Gene Summary This gene encodes an enzyme that hydrolyzes terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides. Its activity is necessary for the catabolism of N-linked carbohydrates released during glycoprotein turnover and it is member of family 38 of glycosyl hydrolases. The full length protein is processed in two steps. First, a 49 aa leader sequence is cleaved off and the remainder of the protein is processed into 3 peptides of 70 kDa, 42 kDa (D) and 13/15 kDa (E). Next, the 70 kDa peptide is further processed into three peptides (A, B and C). The A, B and C peptides are disulfide-linked. Defects in this gene have been associated with lysosomal alpha-mannosidosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2010]

Other Versions

{0} Product Review(s)

0 Product Review(s) Submit review

Be the first one to submit a review

Product Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.