GDI2 (NM_001494) Human Tagged ORF Clone

CAT#: RC200596

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GDI2 (Myc-DDK-tagged)-Human GDP dissociation inhibitor 2 (GDI2), transcript variant 1



  "NM_001494" in other vectors (7)

Reconstitution Protocol

USD 457.00

USD 686.00


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Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol GDI2
Synonyms HEL-S-46e; RABGDIB
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC200596 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATGAGGAGTACGACGTGATCGTGCTGGGCACCGGCCTGACGGAATGTATCCTGTCAGGTATAATGT
CAGTGAATGGCAAGAAAGTTCTTCATATGGATCGAAACCCTTACTACGGAGGAGAGAGTGCATCTATAAC
ACCATTGGAAGATTTATACAAAAGATTTAAAATACCAGGATCACCACCCGAGTCAATGGGGAGAGGAAGA
GACTGGAATGTTGACTTGATTCCCAAGTTCCTTATGGCTAATGGTCAGCTGGTTAAGATGCTGCTTTATA
CAGAGGTAACTCGCTATCTGGATTTTAAAGTGACTGAAGGGAGCTTTGTCTATAAGGGTGGAAAAATCTA
CAAGGTTCCTTCCACTGAAGCAGAAGCCCTGGCATCTAGCCTAATGGGATTGTTTGAAAAACGTCGCTTC
AGGAAATTCCTAGTGTATGTTGCCAACTTCGATGAAAAAGATCCAAGAACTTTTGAAGGCATTGATCCTA
AGAAGACCACAATGCGAGATGTGTATAAGAAATTTGATTTGGGTCAAGACGTTATAGATTTTACTGGTCA
TGCTCTTGCACTTTACAGAACTGATGATTACTTAGATCAACCGTGTTATGAAACCATTAATAGAATTAAA
CTTTACAGTGAATCTTTGGCAAGATATGGCAAAAGCCCATACCTTTATCCACTCTATGGCCTTGGAGAAC
TGCCCCAAGGATTTGCAAGGCTAAGTGCTATTTATGGAGGTACCTATATGCTGAATAAACCCATTGAAGA
AATCATTGTACAGAATGGAAAAGTAATTGGTGTAAAATCTGAAGGAGAAATTGCTCGCTGTAAGCAGCTC
ATCTGTGACCCCAGCTACGTAAAAGATCGGGTAGAAAAAGTGGGCCAGGTGATCAGAGTTATTTGCATCC
TCAGCCACCCCATCAAGAACACCAATGATGCCAACTCCTGCCAGATCATTATTCCACAGAACCAAGTCAA
TCGAAAGTCAGATATCTACGTCTGCATGATCTCCTTTGCGCACAATGTAGCAGCACAAGGGAAGTACATT
GCTATAGTTAGTACAACTGTGGAAACCAAGGAGCCTGAGAAGGAAATCAGACCAGCTTTGGAGCTCTTGG
AACCAATTGAACAGAAATTTGTTAGCATCAGTGACCTCCTGGTACCAAAAGACTTGGGAACAGAAAGCCA
GATCTTTATTTCCCGCACATATGATGCCACCACTCATTTTGAGACAACGTGTGATGACATTAAAAACATC
TATAAGAGGATGACAGGATCAGAGTTTGACTTTGAGGAAATGAAGCGCAAGAAGAATGACATCTATGGGG
AAGAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC200596 protein sequence
Red=Cloning site Green=Tags(s)

MNEEYDVIVLGTGLTECILSGIMSVNGKKVLHMDRNPYYGGESASITPLEDLYKRFKIPGSPPESMGRGR
DWNVDLIPKFLMANGQLVKMLLYTEVTRYLDFKVTEGSFVYKGGKIYKVPSTEAEALASSLMGLFEKRRF
RKFLVYVANFDEKDPRTFEGIDPKKTTMRDVYKKFDLGQDVIDFTGHALALYRTDDYLDQPCYETINRIK
LYSESLARYGKSPYLYPLYGLGELPQGFARLSAIYGGTYMLNKPIEEIIVQNGKVIGVKSEGEIARCKQL
ICDPSYVKDRVEKVGQVIRVICILSHPIKNTNDANSCQIIIPQNQVNRKSDIYVCMISFAHNVAAQGKYI
AIVSTTVETKEPEKEIRPALELLEPIEQKFVSISDLLVPKDLGTESQIFISRTYDATTHFETTCDDIKNI
YKRMTGSEFDFEEMKRKKNDIYGED

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001494
ORF Size 1335 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001494.4
RefSeq Size 2441 bp
RefSeq ORF 1338 bp
Locus ID 2665
UniProt ID P50395
Cytogenetics 10p15.1
Domains GDI
MW 50.7 kDa
Gene Summary GDP dissociation inhibitors are proteins that regulate the GDP-GTP exchange reaction of members of the rab family, small GTP-binding proteins of the ras superfamily, that are involved in vesicular trafficking of molecules between cellular organelles. GDIs slow the rate of dissociation of GDP from rab proteins and release GDP from membrane-bound rabs. GDI2 is ubiquitously expressed. The GDI2 gene contains many repetitive elements indicating that it may be prone to inversion/deletion rearrangements. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]

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