GDI2 (NM_001494) Human Tagged ORF Clone

CAT#: RC200596

GDI2 (Myc-DDK-tagged)-Human GDP dissociation inhibitor 2 (GDI2), transcript variant 1

Product Images

Circular map for RC200596

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  Western blot validation of overexpression lysate (Cat# LY400572) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200596 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified GDI2 protein (Cat# TP300596). The protein was produced from HEK293T cells transfected with GDI2 cDNA clone (Cat# RC200596) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GDI2
• Synonyms: HEL-S-46e; RABGDIB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200596 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGAGGAGTACGACGTGATCGTGCTGGGCACCGGCCTGACGGAATGTATCCTGTCAGGTATAATGT
  CAGTGAATGGCAAGAAAGTTCTTCATATGGATCGAAACCCTTACTACGGAGGAGAGAGTGCATCTATAAC
  ACCATTGGAAGATTTATACAAAAGATTTAAAATACCAGGATCACCACCCGAGTCAATGGGGAGAGGAAGA
  GACTGGAATGTTGACTTGATTCCCAAGTTCCTTATGGCTAATGGTCAGCTGGTTAAGATGCTGCTTTATA
  CAGAGGTAACTCGCTATCTGGATTTTAAAGTGACTGAAGGGAGCTTTGTCTATAAGGGTGGAAAAATCTA
  CAAGGTTCCTTCCACTGAAGCAGAAGCCCTGGCATCTAGCCTAATGGGATTGTTTGAAAAACGTCGCTTC
  AGGAAATTCCTAGTGTATGTTGCCAACTTCGATGAAAAAGATCCAAGAACTTTTGAAGGCATTGATCCTA
  AGAAGACCACAATGCGAGATGTGTATAAGAAATTTGATTTGGGTCAAGACGTTATAGATTTTACTGGTCA
  TGCTCTTGCACTTTACAGAACTGATGATTACTTAGATCAACCGTGTTATGAAACCATTAATAGAATTAAA
  CTTTACAGTGAATCTTTGGCAAGATATGGCAAAAGCCCATACCTTTATCCACTCTATGGCCTTGGAGAAC
  TGCCCCAAGGATTTGCAAGGCTAAGTGCTATTTATGGAGGTACCTATATGCTGAATAAACCCATTGAAGA
  AATCATTGTACAGAATGGAAAAGTAATTGGTGTAAAATCTGAAGGAGAAATTGCTCGCTGTAAGCAGCTC
  ATCTGTGACCCCAGCTACGTAAAAGATCGGGTAGAAAAAGTGGGCCAGGTGATCAGAGTTATTTGCATCC
  TCAGCCACCCCATCAAGAACACCAATGATGCCAACTCCTGCCAGATCATTATTCCACAGAACCAAGTCAA
  TCGAAAGTCAGATATCTACGTCTGCATGATCTCCTTTGCGCACAATGTAGCAGCACAAGGGAAGTACATT
  GCTATAGTTAGTACAACTGTGGAAACCAAGGAGCCTGAGAAGGAAATCAGACCAGCTTTGGAGCTCTTGG
  AACCAATTGAACAGAAATTTGTTAGCATCAGTGACCTCCTGGTACCAAAAGACTTGGGAACAGAAAGCCA
  GATCTTTATTTCCCGCACATATGATGCCACCACTCATTTTGAGACAACGTGTGATGACATTAAAAACATC
  TATAAGAGGATGACAGGATCAGAGTTTGACTTTGAGGAAATGAAGCGCAAGAAGAATGACATCTATGGGG
  AAGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200596 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNEEYDVIVLGTGLTECILSGIMSVNGKKVLHMDRNPYYGGESASITPLEDLYKRFKIPGSPPESMGRGR
  DWNVDLIPKFLMANGQLVKMLLYTEVTRYLDFKVTEGSFVYKGGKIYKVPSTEAEALASSLMGLFEKRRF
  RKFLVYVANFDEKDPRTFEGIDPKKTTMRDVYKKFDLGQDVIDFTGHALALYRTDDYLDQPCYETINRIK
  LYSESLARYGKSPYLYPLYGLGELPQGFARLSAIYGGTYMLNKPIEEIIVQNGKVIGVKSEGEIARCKQL
  ICDPSYVKDRVEKVGQVIRVICILSHPIKNTNDANSCQIIIPQNQVNRKSDIYVCMISFAHNVAAQGKYI
  AIVSTTVETKEPEKEIRPALELLEPIEQKFVSISDLLVPKDLGTESQIFISRTYDATTHFETTCDDIKNI
  YKRMTGSEFDFEEMKRKKNDIYGED
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001494
• ORF Size: 1335 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001494.4
• RefSeq Size: 2441 bp
• RefSeq ORF: 1338 bp
• Locus ID: 2665
• UniProt ID: P50395
• Cytogenetics: 10p15.1
• Domains: GDI
• MW: 50.7 kDa
• Gene Summary: GDP dissociation inhibitors are proteins that regulate the GDP-GTP exchange reaction of members of the rab family, small GTP-binding proteins of the ras superfamily, that are involved in vesicular trafficking of molecules between cellular organelles. GDIs slow the rate of dissociation of GDP from rab proteins and release GDP from membrane-bound rabs. GDI2 is ubiquitously expressed. The GDI2 gene contains many repetitive elements indicating that it may be prone to inversion/deletion rearrangements. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]