PDLIM5 (NM_006457) Human Tagged ORF Clone

CAT#: RC200592

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PDLIM5 (Myc-DDK-tagged)-Human PDZ and LIM domain 5 (PDLIM5), transcript variant 1



  "NM_006457" in other vectors (4)

Reconstitution Protocol

USD 555.00

USD 832.00

In Stock*

Size
    • 10 ug

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Frequently bought together (4)
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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol PDLIM5
Synonyms ENH; ENH1; L9; LIM
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC200592 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCAACTACAGTGTGTCACTGGTTGGCCCAGCTCCTTGGGGTTTCCGGCTGCAGGGCGGTAAGGATT
TCAACATGCCTCTGACAATCTCTAGTCTAAAAGATGGCGGCAAGGCAGCCCAGGCAAATGTAAGAATAGG
CGATGTGGTTCTCAGCATTGATGGAATAAATGCACAAGGAATGACTCATCTTGAAGCCCAGAATAAGATT
AAGGGTTGTACAGGCTCTTTGAATATGACTCTGCAAAGAGCATCTGCTGCACCCAAGCCTGAGCCGGTTC
CTGTTCAAAAGGGAGAACCTAAAGAAGTAGTTAAACCTGTGCCCATTACATCTCCTGCTGTGTCCAAAGT
CACTTCCACAAACAACATGGCCTACAATAAGGCACCACGGCCTTTTGGTTCTGTGTCTTCACCAAAAGTC
ACATCCATCCCATCACCATCGTCTGCCTTCACCCCAGCCCATGCGACCACCTCATCACATGCTTCCCCTT
CACCCGTGGCTGCCGTCACTCCTCCCCTGTTCGCTGCATCTGGACTGCATGCTAATGCCAATCTTAGTGC
TGACCAGTCTCCATCTGCACTGAGCGCTGGTAAAACTGCAGTTAATGTCCCACGGCAGCCCACAGTCACC
AGCGTGTGTTCCGAGACTTCTCAGGAGCTAGCAGAGGGACAGAGAAGAGGATCCCAGGGTGACAGTAAAC
AGCAAAATGGCCCACCAAGAAAACACATTGTGGAGCGCTATACAGAGTTTTATCATGTACCCACTCACAG
TGATGCCAGCAAGAAGAGACTGATTGAGGATACTGAAGACTGGCGTCCAAGGACTGGAACAACTCAGTCT
CGCTCTTTCCGAATCCTTGCCCAGATCACTGGGACTGAACATTTGAAAGAATCTGAAGCCGATAATACAA
AGAAGGCAAATAACTCTCAGGAGCCTTCTCCGCAGTTGGCTTCCTCGGTAGCTTCCACACGGAGCATGCC
CGAGAGCCTGGACAGCCCAACCTCTGGCAGACCAGGGGTTACCAGCCTCACAACTGCAGCTGCCTTCAAG
CCTGTAGGATCCACTGGCGTCATCAAGTCACCAAGCTGGCAACGGCCAAACCAAGGAGTACCTTCCACTG
GAAGAATCTCAAACAGCGCTGCTTACTCAGGATCAGTGGCACCAGCCAACTCAGCTTTGGGACAAACCCA
GCCAAGTGACCAGGACACTTTAGTGCAAAGAGCTGAGCACATTCCAGCAGGGAAACGAACTCCGATGTGC
GCCCATTGTAACCAGGTCATCAGAGGACCATTCTTAGTGGCACTGGGGAAATCTTGGCACCCAGAAGAAT
TCAACTGCGCTCACTGCAAAAATACAATGGCCTACATTGGATTTGTAGAGGAGAAAGGAGCCCTGTATTG
TGAGCTGTGCTATGAGAAATTCTTTGCCCCTGAATGTGGTCGATGCCAAAGGAAGATCCTTGGAGAAGTC
ATCAATGCGTTGAAACAAACTTGGCATGTTTCCTGTTTTGTGTGTGTAGCCTGTGGAAAGCCCATTCGGA
ACAATGTTTTTCACTTGGAGGATGGTGAACCCTACTGTGAGACTGATTATTATGCCCTCTTTGGTACTAT
ATGCCATGGATGTGAATTTCCCATAGAAGCTGGTGACATGTTCCTGGAAGCTCTGGGCTACACCTGGCAT
GACACTTGCTTTGTATGCTCAGTGTGTTGTGAAAGTTTGGAAGGTCAGACCTTTTTCTCCAAGAAGGACA
AGCCCCTGTGTAAGAAACATGCTCATTCTGTGAATTTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC200592 protein sequence
Red=Cloning site Green=Tags(s)

MSNYSVSLVGPAPWGFRLQGGKDFNMPLTISSLKDGGKAAQANVRIGDVVLSIDGINAQGMTHLEAQNKI
KGCTGSLNMTLQRASAAPKPEPVPVQKGEPKEVVKPVPITSPAVSKVTSTNNMAYNKAPRPFGSVSSPKV
TSIPSPSSAFTPAHATTSSHASPSPVAAVTPPLFAASGLHANANLSADQSPSALSAGKTAVNVPRQPTVT
SVCSETSQELAEGQRRGSQGDSKQQNGPPRKHIVERYTEFYHVPTHSDASKKRLIEDTEDWRPRTGTTQS
RSFRILAQITGTEHLKESEADNTKKANNSQEPSPQLASSVASTRSMPESLDSPTSGRPGVTSLTTAAAFK
PVGSTGVIKSPSWQRPNQGVPSTGRISNSAAYSGSVAPANSALGQTQPSDQDTLVQRAEHIPAGKRTPMC
AHCNQVIRGPFLVALGKSWHPEEFNCAHCKNTMAYIGFVEEKGALYCELCYEKFFAPECGRCQRKILGEV
INALKQTWHVSCFVCVACGKPIRNNVFHLEDGEPYCETDYYALFGTICHGCEFPIEAGDMFLEALGYTWH
DTCFVCSVCCESLEGQTFFSKKDKPLCKKHAHSVNF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_006457
ORF Size 1788 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_006457.5
RefSeq Size 6132 bp
RefSeq ORF 1791 bp
Locus ID 10611
UniProt ID Q96HC4
Cytogenetics 4q22.3
Domains PDZ, LIM
Protein Families Druggable Genome
MW 64 kDa
Gene Summary This gene encodes a member of a family of proteins that possess a 100-amino acid PDZ domain at the N terminus and one to three LIM domains at the C-terminus. This family member functions as a scaffold protein that tethers protein kinases to the Z-disk in striated muscles. It is thought to function in cardiomyocyte expansion and in restraining postsynaptic growth of excitatory synapses. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2012]

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