GADD34 (PPP1R15A) (NM_014330) Human Tagged ORF Clone

CAT#: RC200581

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PPP1R15A (Myc-DDK-tagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 15A (PPP1R15A)



  "NM_014330" in other vectors (6)

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 118.00

USD 616.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol PPP1R15A
Synonyms GADD34
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC200581 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCCAGGCCAAGCACCCCATCAGGCTACCCCGTGGAGGGATGCCCACCCTTTCTTCCTCCTGTCCC
CAGTGATGGGCCTCCTCAGCCGCGCCTGGAGCCGCCTGAGGGGCCTGGGACCTCTAGAGCCCTGGCTGGT
GGAAGCAGTAAAAGGAGCAGCTCTGGTAGAAGCTGGCCTGGAGGGAGAAGCTAGGACTCCTCTGGCAATC
CCCCATACCCCTTGGGGCAGACGCCCTGAAGAGGAGGCTGAAGACAGTGGAGGCCCTGGAGAGGACAGAG
AAACACTGGGGCTGAAAACCAGCAGTTCCCTTCCTGAAGCCTGGGGACTTTTGGATGATGATGATGGCAT
GTATGGTGAGCGAGAGGCAACCAGTGTCCCTAGAGGGCAGGGAAGTCAATTTGCAGATGGCCAGCGTGCT
CCCCTGTCTCCCAGCCTTCTGATAAGGACACTGCAAGGTTCTGATAAGAACCCAGGGGAGGAGAAAGCCG
AGGAAGAGGGAGTTGCTGAAGAGGAGGGAGTTAACAAGTTCTCTTATCCACCATCACACCGGGAGTGTTG
TCCAGCCGTGGAGGAGGAGGACGATGAAGAAGCTGTAAAGAAAGAAGCTCACAGAACCTCTACTTCTGCC
TTGTCTCCAGGATCCAAGCCCAGCACTTGGGTGTCTTGCCCAGGGGAGGAAGAGAATCAAGCCACGGAGG
ATAAAAGAACAGAAAGAAGTAAAGGAGCCAGGAAGACCTCCGTGTCCCCCCGATCTTCAGGCTCCGACCC
CAGGTCCTGGGAGTATCGTTCAGGAGAGGCGTCCGAGGAGAAGGAGGAAAAGGCACACAAAGAAACTGGG
AAAGGAGAAGCTGCCCCAGGGCCGCAATCCTCAGCCCCAGCCCAGAGGCCCCAGCTCAAGTCCTGGTGGT
GCCAACCCAGTGATGAAGAGGAGGGTGAGGTCAAGGCTTTGGGGGCAGCTGAGAAGGATGGAGAAGCTGA
GTGTCCTCCCTGCATCCCCCCACCAAGTGCCTTCCTGAAGGCCTGGGTGTATTGGCCAGGAGAGGACACA
GAGGAAGAGGAAGATGAGGAAGAAGATGAGGACAGTGACTCTGGATCAGATGAGGAAGAGGGAGAAGCTG
AGGCTTCCTCTTCCACTCCTGCTACAGGTGTCTTCTTGAAGTCCTGGGTCTATCAGCCAGGAGAGGACAC
AGAGGAGGAGGAAGATGAGGACAGTGATACAGGATCAGCCGAGGATGAAAGAGAAGCTGAGACTTCTGCT
TCCACACCCCCTGCAAGTGCTTTCTTGAAGGCCTGGGTGTATCGGCCAGGAGAGGACACGGAGGAGGAGG
AAGATGAGGATGTGGATAGTGAGGATAAGGAAGATGATTCAGAAGCAGCCTTGGGAGAAGCTGAGTCAGA
CCCACATCCCTCCCACCCGGACCAGAGGGCCCACTTCAGGGGCTGGGGATATCGACCTGGAAAAGAGACA
GAGGAAGAGGAAGCTGCTGAGGACTGGGGAGAAGCTGAGCCCTGCCCCTTCCGAGTGGCCATCTATGTAC
CTGGAGAGAAGCCACCGCCTCCCTGGGCTCCTCCTAGGCTGCCCCTCCGACTGCAAAGGCGGCTCAAGCG
CCCAGAAACCCCTACTCATGATCCGGACCCTGAGACTCCCCTAAAGGCCAGAAAGGTGCGCTTCTCCGAG
AAGGTCACTGTCCATTTCCTGGCTGTCTGGGCAGGGCCGGCCCAGGCCGCCCGCCAGGGCCCCTGGGAGC
AGCTTGCTCGGGATCGCAGCCGCTTCGCACGCCGCATCACCCAGGCCCAGGAGGAGCTGAGCCCCTGCCT
CACCCCTGCTGCCCGGGCCAGAGCCTGGGCACGCCTCAGGAACCCACCTTTAGCCCCCATCCCTGCCCTC
ACCCAGACCTTGCCTTCCTCCTCTGTCCCTTCGTCCCCAGTCCAGACCACGCCCTTGAGCCAAGCTGTGG
CCACACCTTCCCGCTCGTCTGCTGCTGCAGCGGCTGCCCTGGACCTCAGTGGGAGGCGTGGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC200581 protein sequence
Red=Cloning site Green=Tags(s)

MAPGQAPHQATPWRDAHPFFLLSPVMGLLSRAWSRLRGLGPLEPWLVEAVKGAALVEAGLEGEARTPLAI
PHTPWGRRPEEEAEDSGGPGEDRETLGLKTSSSLPEAWGLLDDDDGMYGEREATSVPRGQGSQFADGQRA
PLSPSLLIRTLQGSDKNPGEEKAEEEGVAEEEGVNKFSYPPSHRECCPAVEEEDDEEAVKKEAHRTSTSA
LSPGSKPSTWVSCPGEEENQATEDKRTERSKGARKTSVSPRSSGSDPRSWEYRSGEASEEKEEKAHKETG
KGEAAPGPQSSAPAQRPQLKSWWCQPSDEEEGEVKALGAAEKDGEAECPPCIPPPSAFLKAWVYWPGEDT
EEEEDEEEDEDSDSGSDEEEGEAEASSSTPATGVFLKSWVYQPGEDTEEEEDEDSDTGSAEDEREAETSA
STPPASAFLKAWVYRPGEDTEEEEDEDVDSEDKEDDSEAALGEAESDPHPSHPDQRAHFRGWGYRPGKET
EEEEAAEDWGEAEPCPFRVAIYVPGEKPPPPWAPPRLPLRLQRRLKRPETPTHDPDPETPLKARKVRFSE
KVTVHFLAVWAGPAQAARQGPWEQLARDRSRFARRITQAQEELSPCLTPAARARAWARLRNPPLAPIPAL
TQTLPSSSVPSSPVQTTPLSQAVATPSRSSAAAAAALDLSGRRG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_014330
ORF Size 2022 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_014330.3
RefSeq Size 2399 bp
RefSeq ORF 2025 bp
Locus ID 23645
UniProt ID O75807
Cytogenetics 19q13.33
Protein Families Druggable Genome
MW 73.5 kDa
Gene Summary This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The induction of this gene by ionizing radiation occurs in certain cell lines regardless of p53 status, and its protein response is correlated with apoptosis following ionizing radiation. [provided by RefSeq, Jul 2008]

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