SNRNP40 (NM_004814) Human Tagged ORF Clone

CAT#: RC200548

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SNRNP40 (Myc-DDK-tagged)-Human small nuclear ribonucleoprotein 40kDa (U5) (SNRNP40)



  "NM_004814" in other vectors (4)

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USD 98.00

USD 560.00


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Size
    • 10 ug


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Specifications

Product Data
Product Name SNRNP40 (NM_004814) Human Tagged ORF Clone
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol SNRNP40
Synonyms 40K; HPRP8BP; PRP8BP; PRPF8BP; SPF38; WDR57
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Cell Selection Neomycin
Sequence Data
>RC200548 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATAGAACAGCAGAAGCGTAAGGGCCCAGAGTTGCCGCTGGTTCCAGTCAAGCGGCAGCGGCATGAGT
TGCTGTTGGGAGCGGGGTCTGGCCCAGGAGCCGGGCAGCAGCAGGCGACGCCGGGAGCCTTGCTGCAAGC
GGGACCTCCAAGATGTTCCTCCCTTCAAGCCCCAATCATGCTGCTCTCTGGACATGAAGGGGAAGTCTAC
TGCTGCAAGTTCCACCCCAACGGATCCACCTTAGCATCTGCAGGATTTGACCGACTGATATTACTGTGGA
ATGTCTATGGTGACTGTGATAACTATGCCACACTGAAGGGACACAGTGGAGCAGTGATGGAATTGCATTA
CAACACAGATGGCAGTATGCTTTTCTCAGCATCCACAGATAAAACCGTGGCTGTGTGGGATAGTGAAACA
GGTGAGAGGGTTAAAAGGCTAAAGGGACATACTTCCTTTGTGAATTCCTGTTATCCAGCCAGGAGAGGCC
CTCAGCTTGTCTGCACTGGCAGTGACGATGGCACAGTTAAGCTTTGGGACATCCGGAAGAAAGCAGCCAT
CCAGACATTTCAGAACACGTACCAGGTGTTAGCTGTGACCTTCAATGACACAAGTGATCAGATTATTTCT
GGTGGAATAGACAATGATATCAAGGTCTGGGACCTGCGCCAGAACAAGCTAACCTACACCATGAGAGGCC
ATGCAGATTCAGTGACTGGCCTGAGTTTAAGTTCTGAAGGCTCTTATCTTTTGTCCAATGCAATGGACAA
TACAGTTCGTGTCTGGGATGTCCGGCCATTTGCCCCCAAAGAGAGATGTGTAAAGATATTTCAAGGAAAT
GTGCACAACTTTGAAAAGAACCTTCTGAGATGTTCTTGGTCACCTGATGGAAGCAAAATAGCAGCTGGCT
CAGCCGACAGGTTTGTTTATGTGTGGGATACCACAAGCAGGAGAATATTGTATAAGCTGCCCGGCCATGC
TGGCTCCATCAATGAAGTGGCTTTCCACCCTGATGAGCCCATCATTATCTCAGCATCGAGTGACAAGAGA
CTGTATATGGGAGAGATTCAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC200548 protein sequence
Red=Cloning site Green=Tags(s)

MIEQQKRKGPELPLVPVKRQRHELLLGAGSGPGAGQQQATPGALLQAGPPRCSSLQAPIMLLSGHEGEVY
CCKFHPNGSTLASAGFDRLILLWNVYGDCDNYATLKGHSGAVMELHYNTDGSMLFSASTDKTVAVWDSET
GERVKRLKGHTSFVNSCYPARRGPQLVCTGSDDGTVKLWDIRKKAAIQTFQNTYQVLAVTFNDTSDQIIS
GGIDNDIKVWDLRQNKLTYTMRGHADSVTGLSLSSEGSYLLSNAMDNTVRVWDVRPFAPKERCVKIFQGN
VHNFEKNLLRCSWSPDGSKIAAGSADRFVYVWDTTSRRILYKLPGHAGSINEVAFHPDEPIIISASSDKR
LYMGEIQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_004814
ORF Size 1071 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_004814.3
RefSeq Size 1638 bp
RefSeq ORF 1074 bp
Locus ID 9410
Cytogenetics 1p35.2
Domains WD40
Protein Pathways Spliceosome
MW 39.3 kDa
Gene Summary This gene encodes a component of the U5 small nuclear ribonucleoprotein (snRNP) particle. The U5 snRNP is part of the spliceosome, a multiprotein complex that catalyzes the removal of introns from pre-messenger RNAs. [provided by RefSeq, Jul 2008]

Other Versions

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