SNRNP40 (NM_004814) Human Tagged ORF Clone

CAT#: RC200548

SNRNP40 (Myc-DDK-tagged)-Human small nuclear ribonucleoprotein 40kDa (U5) (SNRNP40)

Product Images

Circular map for RC200548

• 

  Western blot validation of overexpression lysate (Cat# LY417730) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200548 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SNRNP40
• Synonyms: 40K; HPRP8BP; PRP8BP; PRPF8BP; SPF38; WDR57
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200548 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATAGAACAGCAGAAGCGTAAGGGCCCAGAGTTGCCGCTGGTTCCAGTCAAGCGGCAGCGGCATGAGT
  TGCTGTTGGGAGCGGGGTCTGGCCCAGGAGCCGGGCAGCAGCAGGCGACGCCGGGAGCCTTGCTGCAAGC
  GGGACCTCCAAGATGTTCCTCCCTTCAAGCCCCAATCATGCTGCTCTCTGGACATGAAGGGGAAGTCTAC
  TGCTGCAAGTTCCACCCCAACGGATCCACCTTAGCATCTGCAGGATTTGACCGACTGATATTACTGTGGA
  ATGTCTATGGTGACTGTGATAACTATGCCACACTGAAGGGACACAGTGGAGCAGTGATGGAATTGCATTA
  CAACACAGATGGCAGTATGCTTTTCTCAGCATCCACAGATAAAACCGTGGCTGTGTGGGATAGTGAAACA
  GGTGAGAGGGTTAAAAGGCTAAAGGGACATACTTCCTTTGTGAATTCCTGTTATCCAGCCAGGAGAGGCC
  CTCAGCTTGTCTGCACTGGCAGTGACGATGGCACAGTTAAGCTTTGGGACATCCGGAAGAAAGCAGCCAT
  CCAGACATTTCAGAACACGTACCAGGTGTTAGCTGTGACCTTCAATGACACAAGTGATCAGATTATTTCT
  GGTGGAATAGACAATGATATCAAGGTCTGGGACCTGCGCCAGAACAAGCTAACCTACACCATGAGAGGCC
  ATGCAGATTCAGTGACTGGCCTGAGTTTAAGTTCTGAAGGCTCTTATCTTTTGTCCAATGCAATGGACAA
  TACAGTTCGTGTCTGGGATGTCCGGCCATTTGCCCCCAAAGAGAGATGTGTAAAGATATTTCAAGGAAAT
  GTGCACAACTTTGAAAAGAACCTTCTGAGATGTTCTTGGTCACCTGATGGAAGCAAAATAGCAGCTGGCT
  CAGCCGACAGGTTTGTTTATGTGTGGGATACCACAAGCAGGAGAATATTGTATAAGCTGCCCGGCCATGC
  TGGCTCCATCAATGAAGTGGCTTTCCACCCTGATGAGCCCATCATTATCTCAGCATCGAGTGACAAGAGA
  CTGTATATGGGAGAGATTCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200548 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MIEQQKRKGPELPLVPVKRQRHELLLGAGSGPGAGQQQATPGALLQAGPPRCSSLQAPIMLLSGHEGEVY
  CCKFHPNGSTLASAGFDRLILLWNVYGDCDNYATLKGHSGAVMELHYNTDGSMLFSASTDKTVAVWDSET
  GERVKRLKGHTSFVNSCYPARRGPQLVCTGSDDGTVKLWDIRKKAAIQTFQNTYQVLAVTFNDTSDQIIS
  GGIDNDIKVWDLRQNKLTYTMRGHADSVTGLSLSSEGSYLLSNAMDNTVRVWDVRPFAPKERCVKIFQGN
  VHNFEKNLLRCSWSPDGSKIAAGSADRFVYVWDTTSRRILYKLPGHAGSINEVAFHPDEPIIISASSDKR
  LYMGEIQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004814
• ORF Size: 1071 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004814.3
• RefSeq Size: 1638 bp
• RefSeq ORF: 1074 bp
• Locus ID: 9410
• UniProt ID: Q96DI7
• Cytogenetics: 1p35.2
• Domains: WD40
• Protein Pathways: Spliceosome
• MW: 39.3 kDa
• Gene Summary: This gene encodes a component of the U5 small nuclear ribonucleoprotein (snRNP) particle. The U5 snRNP is part of the spliceosome, a multiprotein complex that catalyzes the removal of introns from pre-messenger RNAs. [provided by RefSeq, Jul 2008]